PLK5 (NM_001243079) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	PLK-5; PLK5P; SgK384ps
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC233808 representing NM_001243079 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTACACGGTGCTGACTGGCACCCCACCCTTCATGGCCTCACCCCTGTCGGAGATGTACCAAAACATCC GTGAGGGCCACTACCCCGAACCCGCTCACCTGTCTGCCAATGCGCGCCGCCTCATCGTGCACCTCCTAGC ACCCAACCCGGCCGAGCGGCCCAGCCTGGACCACCTGCTGCAGGACGACTTCTTCACACAGGGTTTCACT CCAGACCGGCTGCCGGCCCACTCCTGCCACAGTCCCCCCATCTTCGCCATACCCCCGCCTCTGGGCAGGA TCTTCCGGAAGGTGGGCCAGCGGCTGCTCACCCAGTGCCGGCCACCCTGCCCCTTCACGCCTAAAGAGGC CTCGGGTCCAGGAGAAGGTGGGCCAGACCCTGACTCCATGGAGTGGGACGGCGAGAGCTCCCTGTCTGCG AAAGAGGTTCCCTGCCTGGAAGGCCCCATCCACCTGGTCGCACAAGGGACCCTGCAGAGTGACCTGGCCG GGCCCGAGGGGAGCCGGCGGCCAGAGGTGGAGGCGGCCCTCAGACACCTGCAGCTGTGCCTGGATGTAGG CCCCCCGGCCACACAGGACCCCCTGGGAGAGCAGCAGCCCATCCTCTGGGCCCCCAAATGGGTGGATTAT TCCAGCAAATACGGCTTTGGCTACCAGCTCTTGGACGGGGGGCGCACGGGACGGCACCCACATGGCCCTG CGACCCCCCGGAGGGAGGGGACCCTCCCCACACCTGTGCCACCTGCTGGACCCGGCCTCTGCCTCCTGCG CTTCCTGGCCTCTGAGCACGCCCTGCTGCTGCTGTTCAGCAATGGGATGGTGCAGGTGAGCTTCAGTGGA GTCCCGGCCCAACTGGTGCTGAGTGGCGAGGGTGAGGGTTTGCAGCTCACCCTCTGGGAGCAGGGGTCCC CTGGCACCTCCTACTCCCTGGACGTCCCGCGGAGCCACGGCTGCGCCCCCACCACCGGACAGCACCTTCA CCACGCCCTCCGCATGCTGCAGAGTATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC233808 representing NM_001243079 Red=Cloning site Green=Tags(s) MYTVLTGTPPFMASPLSEMYQNIREGHYPEPAHLSANARRLIVHLLAPNPAERPSLDHLLQDDFFTQGFT PDRLPAHSCHSPPIFAIPPPLGRIFRKVGQRLLTQCRPPCPFTPKEASGPGEGGPDPDSMEWDGESSLSA KEVPCLEGPIHLVAQGTLQSDLAGPEGSRRPEVEAALRHLQLCLDVGPPATQDPLGEQQPILWAPKWVDY SSKYGFGYQLLDGGRTGRHPHGPATPRREGTLPTPVPPAGPGLCLLRFLASEHALLLLFSNGMVQVSFSG VPAQLVLSGEGEGLQLTLWEQGSPGTSYSLDVPRSHGCAPTTGQHLHHALRMLQSI TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001243079
ORF Size	1008 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001243079.2
RefSeq Size	1933 bp
RefSeq ORF	1011 bp
Locus ID	126520
UniProt ID	Q496M5
Protein Families	Druggable Genome
MW	36.8 kDa
Gene Summary	Inactive serine/threonine-protein kinase that plays a role in cell cycle progression and neuronal differentiation.[UniProtKB/Swiss-Prot Function]

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