C5orf19 (REEP2) (NM_001271803) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	C5orf19; SGC32445; SPG72; Yip2d
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC233562 representing NM_001271803 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGTCCTGGATCATCTCTCGCCTGGTGGTGCTCATCTTTGGCACCCTGTACCCAGCCTATTCTTCCT ACAAGGCCGTGAAGACAAAAAACGTGAAGGAATATGTGAAATGGATGATGTACTGGATCGTCTTTGCCTT CTTCACCACGGCCGAGACGCTCACGGATATAGTGCTCTCCTGGTTCCCCTTCTACTTTGAACTGAAGATC GCCTTCGTGATATGGCTGCTGTCCCCTTACACCAAGGGCTCCAGCGTGCTCTACCGCAAGTTCGTGCACC CAACGCTGTCCAACAAGGAGAAGGAGATCGACGAGTACATCACGCAGGCCCGAGACAAGAGCTATGAGAC CATGATGAGGGTGGGCAAGAGGGGCCTGAACCTTGCCGCCAATGCTGCAGTCACAGCTGCCGCCAAGGGC CAGGGGGTGCTGTCAGAGAAGCTCCGCAGCTTCAGCATGCAGGACCTGACCCTGATCCGGGACGAGGACG CACTGCCCCTGCAGAGGCCTGACGGCCGCCTCCGACCCAGCCCTGGCAGCCTCCTGGACACCATCGAGGA CTTAGGAGATGACCCTGCCCTGAGTCTAAGGTCCAGCACAAACCCGGCAGATTCCCGGACAGAGGCTTCT GAGGATGACATGGGAGACAAAGCTCCCAAGAGGGCCAAACCCATCAAAAAAGCGCCCAAAGCTGAGCCAC TGGCTTCCAAGACACTGAAGACCCGGCCCAAGAAGAAGACCTCTGGCGGGGGCGACTCAGCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC233562 representing NM_001271803 Red=Cloning site Green=Tags(s) MVSWIISRLVVLIFGTLYPAYSSYKAVKTKNVKEYVKWMMYWIVFAFFTTAETLTDIVLSWFPFYFELKI AFVIWLLSPYTKGSSVLYRKFVHPTLSNKEKEIDEYITQARDKSYETMMRVGKRGLNLAANAAVTAAAKG QGVLSEKLRSFSMQDLTLIRDEDALPLQRPDGRLRPSPGSLLDTIEDLGDDPALSLRSSTNPADSRTEAS EDDMGDKAPKRAKPIKKAPKAEPLASKTLKTRPKKKTSGGGDSA TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001271803
ORF Size	762 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001271803.2
RefSeq Size	2209 bp
RefSeq ORF	765 bp
Locus ID	51308
UniProt ID	Q9BRK0
Protein Families	Druggable Genome, Transmembrane
MW	28.9 kDa
Gene Summary	This gene encodes a member of the receptor expression enhancing protein family. Studies of a related gene in mouse suggest that the encoded protein is found in the cell membrane and enhances the function of sweet taste receptors. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2012]

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