RFX4 (NM_001206691) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	NYD-SP10
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC233113 representing NM_001206691 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATCAAAAGGAGAGCCCACCCTGGTGCGGGAGGCGACAGGACCAGGCCTCGACGGCGCCGTTCCACTG AGAGCTGGATTGAAAGATGTCTCAACGAAAGTGAAAACAAACGTTATTCCAGCCACACATCTCTGGGGAA TGTTTCTAATGATGAAAATGAGGAAAAAGAAAATAATAGAGCATCCAAGCCCCACTCCACTCCTGCTACT CTGCAATGGCTGGAGGAGAACTATGAGATTGCAGAGGGGGTCTGCATCCCTCGCAGTGCCCTCTATATGC ATTACCTGGATTTCTGCGAGAAGAATGATACCCAACCTGTCAATGCTGCCAGCTTTGGAAAGATCATAAG GCAGCAGTTTCCTCAGTTAACCACCAGAAGACTCGGGACCCGAGGACAGTCAAAGTACCATTACTATGGC ATTGCAGTGAAAGAAAGCTCCCAATATTATGATGTGATGTATTCCAAGAAAGGAGCTGCCTGGGTGAGTG AGACGGGCAAGAAAGAAGTGAGCAAACAGACAGTGGCATATTCACCCCGGTCCAAACTCGGAACACTGCT GCCAGAATTTCCCAATGTCAAAGATCTAAATCTGCCAGCCAGCCTGCCTGAGGAGAAGGTTTCTACCTTT ATTATGATGTACAGAACACACTGTCAGAGAATACTGGACACTGTAATAAGAGCCAACTTTGATGAGGTTC AAAGTTTCCTTCTGCACTTTTGGCAAGGAATGCCGCCCCACATGCTGCCTGTGCTGGGCTCCTCCACGGT GGTGAACATTGTCGGCGTGTGTGACTCCATCCTCTACAAAGCTATCTCCGGGGTGCTGATGCCCACTGTG CTGCAGGCATTACCTGACAGCTTAACTCAGGTGATTCGAAAGTTTGCCAAGCAACTGGATGAGTGGCTAA AAGTGGCTCTCCACGACCTCCCAGAAAACTTGCGAAACATCAAGTTCGAATTGTCGAGAAGGTTCTCCCA AATTCTGAGACGGCAAACATCACTAAATCATCTCTGCCAGGCATCTCGAACAGTGATCCACAGTGCAGAC ATCACGTTCCAAATGCTGGAAGACTGGAGGAACGTGGACCTGAACAGCATCACCAAGCAAACCCTTTACA CCATGGAAGACTCTCGCGATGAGCACCGGAAACTCATCACCCAATTATATCAGGAGTTTGACCATCTCTT GGAGGAGCAGTCTCCCATCGAGTCCTACATTGAGTGGCTGGATACCATGGTTGACCGCTGTGTTGTGAAG GTGGCTGCCAAGAGACAAGGGTCCTTGAAGAAAGTGGCCCAGCAGTTCCTCTTGATGTGGTCCTGTTTCG GCACAAGGGTGATCCGGGACATGACCTTGCACAGCGCCCCCAGCTTCGGGTCTTTTCACCTAATTCACTT AATGTTTGATGACTACGTGCTCTACCTGTTAGAATCTCTGCACTGTCAGGAGCGGGCCAATGAGCTCATG CGAGCCATGAAGGGAGAAGGAAGCACTGCAGAAGTCCGAGAAGAGATCATCTTGACAGAGGCTGCCGCAC CAACCCCTTCACCAGTGCCATCGTTTTCTCCAGCAAAATCTGCCACATCTGTGGAAGTGCCACCTCCCTC TTCCCCTGTTAGCAATCCTTCCCCTGAGTACACTGGCCTCAGCACTACAGGAGCAATGCAGTCTTACACG TGGTCTCTAACATACACAGTGACGACGGCTGCTGGGTCCCCAGCTGAGAACTCCCAACAGCTGCCCTGTA TGAGGAACACTCATGTGCCTTCTTCCTCCGTCACACACAGGATACCAGTTTATCCCCACAGAGAGGAACA TGGATACACGGGAAGCTATAACTATGGGAGCTATGGCAACCAGCATCCTCACCCCATGCAGAGCCAGTAT CCGGCCCTCCCTCATGACACAGCTATCTCTGGGCCACTCCACTATGCCCCTTACCACAGGAGCTCTGCAC AGTACCCTTTTAATAGCCCCACTTCCCGGATGGAACCTTGTTTGATGAGCAGTACTCCCAGACTGCATCC TACCCCAGTCACTCCCCGCTGGCCAGAGGTGCCCTCAGCCAACACGTGCTACACAAGCCCGTCTGTGCAT TCTGCGAGGTACGGAAACTCTAGTGACATGTATACACCTCTGACAACGCGCAGGAATTCTGAATATGAGC ACATGCAACACTTTCCTGGCTTTGCTTACATCAACGGAGAGGCCTCTACAGGATGGGCTAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC233113 representing NM_001206691 Red=Cloning site Green=Tags(s) MIKRRAHPGAGGDRTRPRRRRSTESWIERCLNESENKRYSSHTSLGNVSNDENEEKENNRASKPHSTPAT LQWLEENYEIAEGVCIPRSALYMHYLDFCEKNDTQPVNAASFGKIIRQQFPQLTTRRLGTRGQSKYHYYG IAVKESSQYYDVMYSKKGAAWVSETGKKEVSKQTVAYSPRSKLGTLLPEFPNVKDLNLPASLPEEKVSTF IMMYRTHCQRILDTVIRANFDEVQSFLLHFWQGMPPHMLPVLGSSTVVNIVGVCDSILYKAISGVLMPTV LQALPDSLTQVIRKFAKQLDEWLKVALHDLPENLRNIKFELSRRFSQILRRQTSLNHLCQASRTVIHSAD ITFQMLEDWRNVDLNSITKQTLYTMEDSRDEHRKLITQLYQEFDHLLEEQSPIESYIEWLDTMVDRCVVK VAAKRQGSLKKVAQQFLLMWSCFGTRVIRDMTLHSAPSFGSFHLIHLMFDDYVLYLLESLHCQERANELM RAMKGEGSTAEVREEIILTEAAAPTPSPVPSFSPAKSATSVEVPPPSSPVSNPSPEYTGLSTTGAMQSYT WSLTYTVTTAAGSPAENSQQLPCMRNTHVPSSSVTHRIPVYPHREEHGYTGSYNYGSYGNQHPHPMQSQY PALPHDTAISGPLHYAPYHRSSAQYPFNSPTSRMEPCLMSSTPRLHPTPVTPRWPEVPSANTCYTSPSVH SARYGNSSDMYTPLTTRRNSEYEHMQHFPGFAYINGEASTGWAK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001206691
ORF Size	2232 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001206691.2
RefSeq Size	3718 bp
RefSeq ORF	2235 bp
Locus ID	5992
UniProt ID	Q33E94
Protein Families	Transcription Factors
MW	84.9 kDa
Gene Summary	This gene is a member of the regulatory factor X gene family, which encodes transcription factors that contain a highly-conserved winged helix DNA binding domain. The protein encoded by this gene is structurally related to regulatory factors X1, X2, X3, and X5. It has been shown to interact with itself as well as with regulatory factors X2 and X3, but it does not interact with regulatory factor X1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2011]

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