RHNO1 (NM_001257098) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	C12orf32; HKMT1188; RHINO
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC232249 representing NM_001257098. Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCCTCCCAGAAAAAAACGCCGCCAGCCTTCCCAGAAAGCCCCGCTGCTGTTCCACCAACAACCACTG GAGGGCCCCAAACACAGCTGTGCATCTACACAGCTTCCCATCACTCACACTCGACAGGTGCCCAGCAAG CCCATTGACCACAGCACCATCACTTCCTGGGTATCACCTGATTTTGATACAGCAGCAGGAAGCTTGTTC CCAGCCTACCAGAAACACCAAAACCGGGCGAGACACTCAAGTCGAAAACCTACCACCTCCAAGTTTCCA CATCTAACTTTTGAGAGTCCGCAATCTTCCAGTTCAGAGACATTGGGGATCCCCTTAATCCGAGAGTGC CCCAGTGAATCAGAAAAGGATGTTTCCAGAAGACCCTTAGTTCCAGTGCTCAGTCCCCAAAGCTGTGGG AACATGTCAGTGCAGGCACTTCAGAGCTTACCTTATGTGTTCATTCCACCTGATATCCAGACCCCAGAG TCATCATCTGTGAAGGAAGAACTCATTCCCCAAGATCAGAAGGAAAACAGCCTTCTAAGCTGCACTCTT CACACTGGCACTCCTAATAGCCCAGAGCCTGGACCTGTTCTGGTTAAAGACACCCCCGAGGACAAGTAT GGAATAAAGGTCACATGGAGGAGACGACAGCACCTGCTTGCTTACCTCAGGGAGAGAGGGAAGCTGAGC AGAAGCCAATTCCTTGTGAAAAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC Protein Sequence (showhide) >Peptide sequence encoded by RC232249 Blue=ORF Red=Cloning site Green=Tag(s) MPPRKKRRQPSQKAPLLFHQQPLEGPKHSCASTQLPITHTRQVPSKPIDHSTITSWVSPDFDTAAGSLF PAYQKHQNRARHSSRKPTTSKFPHLTFESPQSSSSETLGIPLIRECPSESEKDVSRRPLVPVLSPQSCG NMSVQALQSLPYVFIPPDIQTPESSSVKEELIPQDQKENSLLSCTLHTGTPNSPEPGPVLVKDTPEDKY GIKVTWRRRQHLLAYLRERGKLSRSQFLVKS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001257098
ORF Size	714 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq Size	1982 bp
RefSeq ORF	717 bp
Locus ID	83695
UniProt ID	Q9BSD3
MW	26.7 kDa
Gene Summary	Plays a role in DNA damage response (DDR) signaling upon genotoxic stresses such as ionizing radiation (IR) during the S phase. Recruited to sites of DNA damage through interaction with the 9-1-1 cell-cycle checkpoint response complex and TOPBP1 in a ATR-dependent manner. Required for the progression of the G1 to S phase transition. Plays a role in the stimulation of CHEK1 phosphorylation.[UniProtKB/Swiss-Prot Function]

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