SR1 (SRI) (NM_001256891) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	CP-22; CP22; SCN; V19
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC232078 representing NM_001256891 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGTACCCGGGGCATCCTGGCGCCGGCGGCGGGTACTACCCAGGCGGGTATGGAGGGGCTCCCGGAG GGCCTGCGTTTCCCGGACAAACTCAGGATCCGCTGTATGGTTACTTTGCTGCTGTAGCTGGACAGGATGG GCAGATAGATGCTGATGAATTGCAGAGATGTCTGACACAGTCTGGCATTGCTGGAGGATACAAACCTTTT AACCTGGAGACTTGCCGGCTTATGGTTTCAATGCTGGATAGAGATATGTCTGGCACAATGGGTTTCAATG AATTTAAAGAACTCTGGGCTGTACTGAATGGCTGGAGACAACACTTTATCAGTTTTGACACTGACAGGAG TGGAACAGTAGACCCACAAGAATTGCAGAAGGCCCTGACAACAATGGGATTTAGGTTGAGTCCCCAGGCT GTGAATTCAATTGCAAAACGATACAGCACCAATGGAAAGATCACCTTCGACGACTACATCGCCTGCTGCG TCAAACTGAGGGCTCTTACAGACAGCTTTCGAAGACGGGATACTGCTCAGCAAGGTGTTGTGAATTTCCC ATATGATGATGTAAGTCTTAGAAAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC232078 representing NM_001256891 Red=Cloning site Green=Tags(s) MAYPGHPGAGGGYYPGGYGGAPGGPAFPGQTQDPLYGYFAAVAGQDGQIDADELQRCLTQSGIAGGYKPF NLETCRLMVSMLDRDMSGTMGFNEFKELWAVLNGWRQHFISFDTDRSGTVDPQELQKALTTMGFRLSPQA VNSIAKRYSTNGKITFDDYIACCVKLRALTDSFRRRDTAQQGVVNFPYDDVSLRN TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001256891
ORF Size	585 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001256891.1, NP_001243820.1
RefSeq Size	932 bp
RefSeq ORF	588 bp
Locus ID	6717
UniProt ID	P30626
Protein Families	Druggable Genome
MW	21.8 kDa
Gene Summary	This gene encodes a calcium-binding protein with multiple E-F hand domains that relocates from the cytoplasm to the sarcoplasmic reticulum in response to elevated calcium levels. In addition to regulating intracellular calcium homeostasis it also modulates excitation-contraction coupling in the heart. Alternative splicing results in multiple transcript variants encoding distinct proteins. Multiple pseudogenes exist for this gene. [provided by RefSeq, Mar 2012]

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