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MAP3K12 (NM_001193511) Human Tagged ORF Clone
CAT＃: RC231434
- TrueORF®
MAP3K12 (Myc-DDK-tagged)-Human mitogen-activated protein kinase kinase kinase 12 (MAP3K12), transcript variant 1
ORF Plasmid: tGFP
"NM_001193511" in other vectors (3)
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CNY 9,800.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | DLK; MEKK12; MUK; ZPK; ZPKP1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC231434 representing NM_001193511
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTTGCCTCCATGAGACCCGAACACCCTCTCCTTCCTTTGGGGGCTTTGTGTCTACCCTAAGTGAGG CATCCATGCGCAAGCTGGACCCAGACACTTCTGACTGCACTCCCGAGAAGGACCTGACGCCTACCCAGTG TGTACTTCGAGATGTGGTACCCCTTGGTGGGCAGGGTGGGGGAGGGCCCAGCCCCTCCCCAGGTGGAGAG CCGCCCCCTGAGCCTTTTGCCAACAGTGTCCTGCAGCTACATGAGCAGGATGCAGGGGGCCCAGGGGGAG CAGCTGGGTCACCTGAGAGTCGGGCATCCAGAGTTCGAGCTGACGAGGTGCGACTGCAGTGCCAGAGTGG CAGTGGCTTCCTTGAGGGCCTCTTTGGCTGCCTGCGCCCTGTCTGGACCATGATTGGCAAAGCCTACTCC ACTGAGCACAAGCAGCAGCAGGAAGACCTTTGGGAGGTCCCCTTTGAGGAAATCCTGGACCTGCAGTGGG TGGGCTCAGGGGCCCAGGGTGCTGTCTTCCTGGGGCGCTTCCACGGGGAGGAGGTGGCTGTGAAGAAGGT GCGAGACCTCAAAGAAACCGACATCAAGCACTTGCGAAAGCTGAAGCACCCCAACATCATCACTTTCAAG GGTGTGTGCACCCAGGCTCCCTGCTACTGCATCCTCATGGAGTTCTGCGCCCAGGGCCAGCTGTATGAGG TACTGCGGGCTGGCCGCCCTGTCACCCCCTCCTTACTGGTTGACTGGTCCATGGGCATCGCTGGTGGCAT GAACTACCTGCACCTGCACAAGATTATCCACAGGGATCTCAAGTCACCCAACATGCTAATCACCTACGAC GATGTGGTGAAGATCTCAGATTTTGGCACTTCCAAGGAGCTGAGTGACAAGAGCACCAAGATGTCCTTTG CAGGGACAGTAGCCTGGATGGCCCCTGAGGTGATCCGCAATGAACCTGTGTCTGAGAAGGTCGACATCTG GTCCTTTGGCGTGGTGCTATGGGAACTGCTGACTGGTGAGATCCCCTACAAAGACGTAGATTCCTCAGCC ATTATCTGGGGTGTGGGAAGCAACAGTCTCCATCTGCCCGTGCCCTCCAGTTGCCCAGATGGTTTCAAGA TCCTGCTTCGCCAGTGCTGGAATAGCAAACCACGAAATCGCCCATCATTCCGACAGATCCTGCTGCATCT GGACATTGCCTCAGCTGATGTACTCTCCACACCCCAGGAGACTTACTTTAAGTCCCAGGCAGAGTGGCGG GAAGAAGTAAAACTGCACTTTGAAAAGATTAAGTCAGAAGGGACCTGTCTGCACCGCCTAGAAGAGGAAC TGGTGATGAGGAGGAGGGAGGAGCTCAGACACGCCCTGGACATCAGGGAGCACTATGAAAGGAAGCTGGA GAGAGCCAACAACCTGTATATGGAACTTAATGCCCTCATGTTGCAGCTGGAACTCAAGGAGAGGGAGCTG CTCAGGCGAGAGCAAGCTTTAGAGCGGAGGTGCCCAGGCCTGCTGAAGCCACACCCTTCCCGGGGCCTCC TGCATGGAAACACAATGGAGAAGCTTATCAAGAAGAGGAATGTGCCACAGAAGCTGTCACCCCATAGCAA AAGGCCAGATATCCTCAAGACGGAGTCTTTGCTCCCTAAACTAGATGCAGCCCTGAGTGGGGTGGGGCTT CCTGGGTGTCCTAAGGGCCCCCCCTCACCAGGACGGAGTCGCCGTGGCAAGACCCGTCACCGCAAGGCCA GCGCCAAGGGGAGCTGTGGGGACCTGCCTGGGCTTCGTACAGCTGTGCCACCCCATGAACCTGGAGGACC AGGAAGCCCAGGGGGCCTAGGAGGGGGACCCTCAGCCTGGGAGGCCTGCCCTCCCGCCCTCCGTGGGCTT CATCATGACCTCCTGCTCCGCAAAATGTCTTCATCGTCCCCAGACCTGCTGTCAGCAGCACTAGGGTCCC GGGGCCGGGGGGCCACAGGCGGAGCTGGGGATCCTGGCTCACCACCTCCGGCCCGGGGTGACACCCCACC AAGTGAGGGCTCAGCCCCTGGCTCCACCAGCCCAGATTCACCTGGGGGAGCCAAAGGGGAACCACCTCCT CCAGTAGGGCCTGGTGAAGGTGTGGGGCTTCTGGGAACTGGAAGGGAAGGGACCTCAGGCCGGGGAGGAA GCCGGGCTGGGTCCCAGCACTTGACCCCAGCTGCACTGCTGTACAGGGCTGCCGTCACCCGAAGTCAGAA ACGTGGCATCTCATCGGAAGAGGAGGAAGGAGAGGTAGACAGTGAAGTAGAGCTGACATCAAGCCAGAGG TGGCCTCAGAGCCTGAACATGCGCCAGTCACTATCTACCTTCAGCTCAGAGAATCCATCAGATGGGGAGG AAGGCACAGCTAGTGAACCTTCCCCCAGTGGCACACCTGAAGTTGGCAGCACCAACACTGATGAGCGGCC AGATGAGCGGTCTGATGACATGTGCTCCCAGGGCTCAGAAATCCCACTGGACCCACCTCCTTCAGAGGTC ATCCCTGGCCCTGAACCCAGCTCCCTGCCCATTCCACACCAGGAACTTCTCAGAGAGCGGGGCCCTCCCA ATTCTGAGGACTCAGACTGTGACAGCACTGAATTGGACAACTCCAACAGCGTTGATGCCTTGCGGCCCCC AGCTTCCCTCCCTCCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC231434 representing NM_001193511
Red=Cloning site Green=Tags(s) MACLHETRTPSPSFGGFVSTLSEASMRKLDPDTSDCTPEKDLTPTQCVLRDVVPLGGQGGGGPSPSPGGE PPPEPFANSVLQLHEQDAGGPGGAAGSPESRASRVRADEVRLQCQSGSGFLEGLFGCLRPVWTMIGKAYS TEHKQQQEDLWEVPFEEILDLQWVGSGAQGAVFLGRFHGEEVAVKKVRDLKETDIKHLRKLKHPNIITFK GVCTQAPCYCILMEFCAQGQLYEVLRAGRPVTPSLLVDWSMGIAGGMNYLHLHKIIHRDLKSPNMLITYD DVVKISDFGTSKELSDKSTKMSFAGTVAWMAPEVIRNEPVSEKVDIWSFGVVLWELLTGEIPYKDVDSSA IIWGVGSNSLHLPVPSSCPDGFKILLRQCWNSKPRNRPSFRQILLHLDIASADVLSTPQETYFKSQAEWR EEVKLHFEKIKSEGTCLHRLEEELVMRRREELRHALDIREHYERKLERANNLYMELNALMLQLELKEREL LRREQALERRCPGLLKPHPSRGLLHGNTMEKLIKKRNVPQKLSPHSKRPDILKTESLLPKLDAALSGVGL PGCPKGPPSPGRSRRGKTRHRKASAKGSCGDLPGLRTAVPPHEPGGPGSPGGLGGGPSAWEACPPALRGL HHDLLLRKMSSSSPDLLSAALGSRGRGATGGAGDPGSPPPARGDTPPSEGSAPGSTSPDSPGGAKGEPPP PVGPGEGVGLLGTGREGTSGRGGSRAGSQHLTPAALLYRAAVTRSQKRGISSEEEEGEVDSEVELTSSQR WPQSLNMRQSLSTFSSENPSDGEEGTASEPSPSGTPEVGSTNTDERPDERSDDMCSQGSEIPLDPPPSEV IPGPEPSSLPIPHQELLRERGPPNSEDSDCDSTELDNSNSVDALRPPASLPP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001193511 |
| ORF Size | 2676 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001193511.2 |
| RefSeq Size | 3640 bp |
| RefSeq ORF | 2679 bp |
| Locus ID | 7786 |
| UniProt ID | Q12852 |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | MAPK signaling pathway |
| MW | 96.8 kDa |
| Gene Summary | This gene encodes a member of the serine/threonine protein kinase family. This kinase contains a leucine-zipper domain and is predominately expressed in neuronal cells. The phosphorylation state of this kinase in synaptic terminals was shown to be regulated by membrane depolarization via calcineurin. This kinase forms heterodimers with leucine zipper containing transcription factors, such as cAMP responsive element binding protein (CREB) and MYC, and thus may play a regulatory role in PKA or retinoic acid induced neuronal differentiation. Alternatively spliced transcript variants encoding different proteins have been described.[provided by RefSeq, Jul 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC231434L3 | Lenti-ORF clone of MAP3K12 (Myc-DDK-tagged)-Human mitogen-activated protein kinase kinase kinase 12 (MAP3K12), transcript variant 1 |
CNY 9,030.00 |
|
| RC231434L4 | Lenti-ORF clone of MAP3K12 (mGFP-tagged)-Human mitogen-activated protein kinase kinase kinase 12 (MAP3K12), transcript variant 1 |
CNY 9,030.00 |
|
| RG231434 | MAP3K12 (tGFP-tagged) - Human mitogen-activated protein kinase kinase kinase 12 (MAP3K12), transcript variant 1 |
CNY 7,890.00 |
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