NT5C1B (NT5C1B-RDH14) (NM_001199103) Human Tagged ORF Clone
CAT#: RC231355
NT5C1B (Myc-DDK-tagged)-Human NT5C1B-RDH14 readthrough (NT5C1B-RDH14), transcript variant 1
ORF Plasmid: tGFP
"NM_001199103" in other vectors (3)
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CNY 4,840.00
CNY 5,700.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | AIRP; cN-IB; cN1B; NT5C1B |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC231355 representing NM_001199103
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTCAAACATCTCTCAAACAGAAAAAGAATGAGCCTGGAATGAGGTCCTCAAAAGAGAGTCTAGAAG CAGAAAAAAGAAAGGAATCTGACAAAACAGGAGTTCGTCTGAGCAATCAGGGATCACAAGAATCATCACT GCGGAAGACAGACTCTCGAGGGTACCTTGTGCGCAGTCAATGGTCTAGAATATCCCGGAGCCCATCCACC AAGGCTCCATCCATAGATGAGCCTAGAAGCAGGAACACCAGTGCTAAGGTAGAGCTCCCCAGCAGCTCCA CGAGCTCCCGGACTCCATCCACCTCCCCAAGCCTGCATGACTCCTCACCGCCGCCGCTGTCCGGGCAGCC CTCGCTCCAGCCACCCGCGTCGCCCCAGCTGCCCCGGTCGCTGGACTCGCGGCCTCCCACGCCCCCAGAG CCCGATCCTGGCTCCCGGCGCAGCACCAAAATGCAAGAGAATCCGGAGGCCTGGGCCCAAGGCATCGTGC GGGAAATCCGCCAGACCCGGGACTCGCAGCCGCTGGAATATTCGCGCACGTCCCCCACCGAGTGGAAGTC CTCCAGCCAGCGCAGGGGGATCTACCCCGCCTCCACCCAGCTGGACCGCAACTCTCTGTCCGAGCAGCAG CAGCAGCAGCGGGAGGACGAGGACGACTACGAGGCTGCCTACTGGGCATCCATGAGGTCGTTCTACGAGA AGAACCCGAGCTGCTCGCGCCCCTGGCCGCCCAAACCCAAGAACGCCATCACCATTGCTCTCTCATCCTG CGCGCTCTTCAACATGGTGGACGGCAGGAAAATCTACGAGCAAGAGGGTCTGGAAAAGTACATGGAGTAT CAGCTCACCAATGAGAACGTCATCCTGACCCCGGGCCCGGCGTTCCGCTTCGTCAAGGCACTACAGTATG TCAATGCTAGACTCCGTGATCTATATCCTGATGAACAGGACTTATTTGATATTGTACTGATGACTAATAA CCATGCCCAAGTGGGAGTGCGGCTTATAAACAGCGTCAATCACTACGGCTTACTGATTGACCGCTTCTGT CTGACCGGGGGAAAAGACCCCATTGGCTATTTGAAGGCATATCTTACCAACTTGTATATTGCTGCAGATT CTGAAAAAGTGCAAGAGGCAATACAAGAAGGTATTGCCTCTGCGACAATGTTTGATGGAGCCAAAGACAT GGCTTACTGTGACACTCAGCTCCGTGTAGCCTTTGATGGGGATGCTGTCCTCTTCTCTGATGAGTCTGAA CATTTTACCAAGGAGCATGGGCTCGACAAATTCTTCCAGTATGATACATTATGTGAAAGTAAGCCTCTTG CTCAGGAAGAGCCTAGGCTGGATGTCTTGATCAATAACGCAGGGATCTTCCAGTGCCCTTACATGAAGAC TGAAGATGGGTTTGAGATGCAGTTCGGAGTGAACCATCTGGGGCACTTTCTACTCACCAATCTTCTCCTT GGACTCCTCAAAAGTTCAGCTCCCAGCAGGATTGTGGTAGTTTCTTCCAAACTTTATAAATACGGAGACA TCAATTTTGATGACTTGAACAGTGAACAAAGCTATAATAAAAGCTTTTGTTATAGCCGGAGCAAACTGGC TAACATTCTTTTTACCAGGGAACTAGCCCGCCGCTTAGAAGGCACAAATGTCACCGTCAATGTGTTGCAT CCTGGTATTGTACGGACAAATCTGGGGAGGCACATACACATTCCACTGTTGGTCAAACCACTCTTCAATT TGGTGTCATGGGCTTTTTTCAAAACTCCAGTAGAAGGTGCCCAGACTTCCATTTATTTGGCCTCTTCACC TGAGGTAGAAGGAGTGTCAGGAAGATACTTTGGGGATTGTAAAGAGGAAGAACTGTTGCCCAAAGCTATG GATGAATCTGTTGCAAGAAAACTCTGGGATATCAGTGAAGTGATGGTTGGCCTGCTAAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC231355 representing NM_001199103
Red=Cloning site Green=Tags(s) MSQTSLKQKKNEPGMRSSKESLEAEKRKESDKTGVRLSNQGSQESSLRKTDSRGYLVRSQWSRISRSPST KAPSIDEPRSRNTSAKVELPSSSTSSRTPSTSPSLHDSSPPPLSGQPSLQPPASPQLPRSLDSRPPTPPE PDPGSRRSTKMQENPEAWAQGIVREIRQTRDSQPLEYSRTSPTEWKSSSQRRGIYPASTQLDRNSLSEQQ QQQREDEDDYEAAYWASMRSFYEKNPSCSRPWPPKPKNAITIALSSCALFNMVDGRKIYEQEGLEKYMEY QLTNENVILTPGPAFRFVKALQYVNARLRDLYPDEQDLFDIVLMTNNHAQVGVRLINSVNHYGLLIDRFC LTGGKDPIGYLKAYLTNLYIAADSEKVQEAIQEGIASATMFDGAKDMAYCDTQLRVAFDGDAVLFSDESE HFTKEHGLDKFFQYDTLCESKPLAQEEPRLDVLINNAGIFQCPYMKTEDGFEMQFGVNHLGHFLLTNLLL GLLKSSAPSRIVVVSSKLYKYGDINFDDLNSEQSYNKSFCYSRSKLANILFTRELARRLEGTNVTVNVLH PGIVRTNLGRHIHIPLLVKPLFNLVSWAFFKTPVEGAQTSIYLASSPEVEGVSGRYFGDCKEEELLPKAM DESVARKLWDISEVMVGLLK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001199103 |
ORF Size | 1950 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001199103.1, NP_001186032.1 |
RefSeq ORF | 1953 bp |
Locus ID | 100526794 |
MW | 73.7 kDa |
Gene Summary | This locus represents naturally occurring read-through transcription between the neighboring NT5C1B (5'-nucleotidase, cytosolic IB) and RDH14 (retinol dehydrogenase 14) genes on chromosome 2. Alternative splicing results in multiple transcript variants, one of which encodes a fusion protein that shares sequence identity with the products of each individual gene. [provided by RefSeq, Nov 2010] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC231355L3 | Lenti-ORF clone of NT5C1B (Myc-DDK-tagged)-Human NT5C1B-RDH14 readthrough (NT5C1B-RDH14), transcript variant 1 |
CNY 7,600.00 |
|
RC231355L4 | Lenti-ORF clone of NT5C1B (mGFP-tagged)-Human NT5C1B-RDH14 readthrough (NT5C1B-RDH14), transcript variant 1 |
CNY 7,600.00 |
|
RG231355 | NT5C1B (tGFP-tagged) - Human NT5C1B-RDH14 readthrough (NT5C1B-RDH14), transcript variant 1 |
CNY 6,270.00 |