NFS1 (NM_001198989) Human Tagged ORF Clone
CAT#: RC231213
NFS1 (Myc-DDK-tagged)-Human NFS1 nitrogen fixation 1 homolog (S. cerevisiae) (NFS1), transcript variant 3
ORF Plasmid: tGFP
"NM_001198989" in other vectors (3)
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CNY 3,656.00
CNY 3,990.00
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | COXPD52; HUSSY-08; IscS; NIFS |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC231213 representing NM_001198989
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGCTCCGAGCCGCTTGGAGGCGGGCGGCAGTGGCGGTGACAGCGGCTCCAGGGCCGAAGCCCGCGG CGCCCACTCGGGGGCTGCGCCTGCGCGTTGGAGACCGTGCTCCTCAGTCTGCGGTTCCCGCAGATACAGC CGCTGCCCCGGAGGTGGGGCCAGTGCTGCGACCTCTCTATATGGATGTGCAAGCTACAACTCCTCTGGAC CCCCGGGTGCTTGATGCCATGCTCCCTTACCTAATCAACTACTATGGGAACCCACACTCCCGGACACATG CTTATGGCTGGGAGAGTGAGGCAGCCATGGAACGTGCTCGTCAGCAAGTAGCATCTCTGATTGGAGCTGA TCCTCGTGAGATCATTTTTACTAGTGGTGCTACTGAATCCAACAACATAGCAATTAAGGAACTAGAGGCT GCTATCCAGCCAGATACTAGCCTGGTGTCAGTCATGACTGTGAACAATGAGATTGGAGTGAAGCAGCCTA TTGCAGAAATAGGGCGGATTTGCAGTTCCAGAAAGGTATATTTCCATACTGATGCAGCCCAGGCTGTTGG AAAAATCCCACTTGATGTCAATGACATGAAAATTGATCTCATGAGCATTAGTGGTCACAAAATCTACGGT CCCAAAGGGGTTGGTGCCATCTACATCCGTCGCCGGCCCCGTGTGCGTGTGGAGGCCCTGCAGAGTGGAG GGGGGCAGGAGCGGGGTATGCGGTCTGGGACAGTGCCCACACCCTTAGTGGTGGGGCTGGGGGCTGCGTG TGAGGTGGCACAGCAAGAGATGGAGTATGACCACAAGCGAATCTCAAAGTTGTCAGAGCGGCTGATACAG AATATAATGAAGAGCCTTCCAGATGTGGTGATGAATGGGGACCCTAAGCACCATTATCCCGGCTGTATCA ACCTCTCCTTTGCATATGTGGAAGGGGAAAGTCTGCTGATGGCACTGAAGGACGTTGCCTTATCCTCAGG GAGTGCCTGCACCTCTGCATCCCTGGAGCCCTCTTATGTGCTTAGAGCAATTGGCACTGATGAGGATTTA GCGCACTCTTCTATCAGGTTTGGAATTGGCCGCTTCACTACAGAGGAGGAAGTGGACTACACAGTGGAGA AATGCATTCAGCATGTGAAGCGTCTTCGAGAAATGAGCCCTCTCTGGGAGATGGTTCAGGATGGCATTGA CCTCAAGAGCATCAAGTGGACCCAACAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC231213 representing NM_001198989
Red=Cloning site Green=Tags(s) MLLRAAWRRAAVAVTAAPGPKPAAPTRGLRLRVGDRAPQSAVPADTAAAPEVGPVLRPLYMDVQATTPLD PRVLDAMLPYLINYYGNPHSRTHAYGWESEAAMERARQQVASLIGADPREIIFTSGATESNNIAIKELEA AIQPDTSLVSVMTVNNEIGVKQPIAEIGRICSSRKVYFHTDAAQAVGKIPLDVNDMKIDLMSISGHKIYG PKGVGAIYIRRRPRVRVEALQSGGGQERGMRSGTVPTPLVVGLGAACEVAQQEMEYDHKRISKLSERLIQ NIMKSLPDVVMNGDPKHHYPGCINLSFAYVEGESLLMALKDVALSSGSACTSASLEPSYVLRAIGTDEDL AHSSIRFGIGRFTTEEEVDYTVEKCIQHVKRLREMSPLWEMVQDGIDLKSIKWTQH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001198989 |
ORF Size | 1218 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001198989.2 |
RefSeq ORF | 1221 bp |
Locus ID | 9054 |
UniProt ID | Q9Y697 |
Protein Pathways | Thiamine metabolism |
MW | 44.8 kDa |
Gene Summary | Iron-sulfur clusters are required for the function of many cellular enzymes. The proteins encoded by this gene supply inorganic sulfur to these clusters by removing the sulfur from cysteine, creating alanine in the process. This gene uses alternate in-frame translation initiation sites to generate mitochondrial forms and cytoplasmic/nuclear forms. Selection of the alternative initiation sites is determined by the cytosolic pH. The encoded proteins belong to the class-V family of pyridoxal phosphate-dependent aminotransferases. Alternatively spliced transcript variants have been described. [provided by RefSeq, Nov 2010] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC231213L3 | Lenti ORF clone of Human NFS1 nitrogen fixation 1 homolog (S. cerevisiae) (NFS1), transcript variant 3, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC231213L4 | Lenti ORF clone of Human NFS1 nitrogen fixation 1 homolog (S. cerevisiae) (NFS1), transcript variant 3, mGFP tagged |
CNY 5,890.00 |
|
RG231213 | NFS1 (tGFP-tagged) - Human NFS1 nitrogen fixation 1 homolog (S. cerevisiae) (NFS1), transcript variant 3 |
CNY 4,370.00 |