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DENND1B (NM_001195216) Human Tagged ORF Clone
CAT＃: RC230758
- TrueORF®
DENND1B (Myc-DDK-tagged)-Human DENN/MADD domain containing 1B (DENND1B), transcript variant 4
ORF Plasmid: tGFP
"NM_001195216" in other vectors (3)
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CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | C1ORF18; C1orf218; FAM31B |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC230758 representing NM_001195216
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCAGCTCCACGGGAGGAGAAAAGATGGCCCCAACCTGTATTTTCGAATCCTGTGGTATTGTGGA AATTCCCAGAGGACTTTGGAGACCAGGAAATACTACAGAGTGTGCCAAAGTTCTGTTTTCCCTTTGACGT TGAAAGGGTGTCTCAGAATCAAGTTGGACAGCACTTTACCTTTGTACTGACAGACATTGAAAGTAAACAG AGATTTGGATTCTGCAGACTGACGTCAGGAGGCACAATTTGTTTATGCATCCTTAGTTACCTTCCCTGGT TTGAAGTGTATTACAAGCTTCTAAATACTCTTGCAGATTACTTGGCTAAGGAACTGGAAAATGATTTGAA TGAAACTCTCAGATCACTGTATAACCACCCAGTACCAAAGGCAAATACTCCTGTAAATTTGAGTGTGCAT TCCTACTTCATTGCCCCTGATGTAACTGGACTCCCAACAATACCCGAGAGTAGAAATCTTACAGAATATT TTGTTGCCGTGGATGTGAACAACATGCTGCAGCTGTATGCCAGTATGCTGCATGAAAGGCGCATCGTGAT TATCTCGAGCAAATTAAGCACTTTAACTGCCTGTATCCATGGATCAGCTGCTCTTCTATACCCAATGTAT TGGCAACACATATACATCCCAGTGCTTCCTCCACACCTGCTGGACTACTGCTGTGCCCCAATGCCATACC TGATTGGAATACACTCCAGCCTCATAGAGAGAGTGAAAAACAAATCATTGGAAGATGTTGTTATGTTAAA TGTTGATACAAACACATTAGAATCACCATTTAGTGACTTGAACAACCTACCAAGTGATGTGGTCTCGGCC TTGAAAAATAAACTGAAGAAGCAGTCTACAGCTACGGGTGATGGAGTAGCTAGGGCCTTTCTTAGAGCAC AGGCTGCTTTGTTTGGATACTACAGAGATGCACTGAGATACAAACCTGGTGAGCCCATCACTTTCTGTGA GGAGAGTTTTGTAAAGCACCGCTCAAGCGTGATGAAACAGTTCCTGGAAACTGCCATTAACCTCCAGCTT TTTAAGCAGTTTATCGATGGTCGACTGGCAAAACTAAATGCAGGAAGGGGTTTCTCTGATGTATTTGAAG AAGAGATCACTTCAGGTGGCTTTTGTGGAGGTAAAGACAAGTTACAATATGATTATCCATTTTCTCAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC230758 representing NM_001195216
Red=Cloning site Green=Tags(s) MAAAPREEKRWPQPVFSNPVVLWKFPEDFGDQEILQSVPKFCFPFDVERVSQNQVGQHFTFVLTDIESKQ RFGFCRLTSGGTICLCILSYLPWFEVYYKLLNTLADYLAKELENDLNETLRSLYNHPVPKANTPVNLSVH SYFIAPDVTGLPTIPESRNLTEYFVAVDVNNMLQLYASMLHERRIVIISSKLSTLTACIHGSAALLYPMY WQHIYIPVLPPHLLDYCCAPMPYLIGIHSSLIERVKNKSLEDVVMLNVDTNTLESPFSDLNNLPSDVVSA LKNKLKKQSTATGDGVARAFLRAQAALFGYYRDALRYKPGEPITFCEESFVKHRSSVMKQFLETAINLQL FKQFIDGRLAKLNAGRGFSDVFEEEITSGGFCGGKDKLQYDYPFSQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001195216 |
| ORF Size | 228 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001195216.1, NP_001182145.1 |
| RefSeq ORF | 231 bp |
| Locus ID | 163486 |
| UniProt ID | Q6P3S1 |
| Protein Families | Transmembrane |
| MW | 44.98 kDa |
| Gene Summary | Clathrin (see MIM 118955)-mediated endocytosis is a major mechanism for internalization of proteins and lipids. Members of the connecdenn family, such as DENND1B, function as guanine nucleotide exchange factors (GEFs) for the early endosomal small GTPase RAB35 (MIM 604199) and bind to clathrin and clathrin adaptor protein-2 (AP2; see MIM 601024). Thus, connecdenns link RAB35 activation with the clathrin machinery (Marat and McPherson, 2010 [PubMed 20154091]).[supplied by OMIM, Nov 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC230758L3 | Lenti-ORF clone of DENND1B (Myc-DDK-tagged)-Human DENN/MADD domain containing 1B (DENND1B), transcript variant 4 |
CNY 5,890.00 |
|
| RC230758L4 | Lenti-ORF clone of DENND1B (mGFP-tagged)-Human DENN/MADD domain containing 1B (DENND1B), transcript variant 4 |
CNY 5,890.00 |
|
| RG230758 | DENND1B (tGFP-tagged) - Human DENN/MADD domain containing 1B (DENND1B), transcript variant 4 |
CNY 4,370.00 |
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