United States
Germany
Japan
United Kingdom
China
SMG7 (NM_001174061) Human Tagged ORF Clone
CAT＃: RC230659
- TrueORF®
SMG7 (Myc-DDK-tagged)-Human Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (SMG7), transcript variant 5
ORF Plasmid: tGFP
"NM_001174061" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 7,976.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | C1orf16; EST1C; SGA56M |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC230659 representing NM_001174061
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTAGTTACCGATTTGGAATACGCTTTAGACAAGAAAGTAGAACAGGATCTCTGGAATCACGCCTTTA AGAATCAGATCACAACACTGCAAGGCCAGGCAAAGAATCGAGCAAATCCGAATCGGAGTGAAGTTCAGGC AAACCTTTCTCTGTTCCTAGAGGCAGCTAGTGGCTTCTATACTCAGTTATTACAAGAACTGTGTACAGTA TTTAATGTAGATTTACCATGCCGTGTGAAGTCTTCCCAATTGGGAATTATCAGCAATAAACAGACGCATA CCAGCGCCATAGTGAAGCCACAGTCTAGCTCCTGTTCCTATATCTGCCAGCACTGCCTCGTCCACCTTGG AGACATTGCTCGATACAGAAACCAGACCAGCCAGGCAGAGTCCTACTATAGGCATGCAGCTCAGCTTGTC CCCTCCAATGGTCAGCCTTATAATCAGTTGGCTATCTTAGCTTCTTCCAAAGGAGACCATCTGACCACAA TTTTCTACTACTGCAGAAGCATTGCTGTGAAGTTCCCTTTCCCAGCTGCCTCCACTAATCTGCAAAAAGC ACTTTCTAAAGCACTGGAAAGCCGAGATGAGGTGAAAACCAAGTGGGGTGTTTCTGACTTCATCAAGGCC TTTATTAAATTCCACGGTCATGTGTACCTGAGTAAGAGCTTGGAAAAGTTGAGCCCTCTTCGAGAGAAAT TGGAAGAACAGTTTAAGAGGCTGCTATTCCAAAAAGCTTTCAACTCTCAGCAGTTAGTTCATGTCACTGT CATTAACCTGTTTCAACTTCATCACCTTCGTGACTTTAGCAATGAAACCGAGCAGCACACTTATAGCCAA GATGAGCAGCTATGTTGGACACAGTTGCTGGCCCTCTTTATGTCTTTTCTTGGCATCCTGTGCAAGTGTC CTCTACAGAATGAGTCTCAGGAGGAGTCCTACAATGCCTATCCTCTTCCAGCAGTCAAGGTCTCCATGGA CTGGCTAAGACTCAGACCCAGGGTCTTTCAGGAGGCAGTGGTGGATGAAAGACAGTACATTTGGCCCTGG TTGATTTCTCTTCTGAATAGTTTCCATCCCCATGAAGAGGACCTCTCAAGTATTAGTGCGACACCACTTC CAGAGGAGTTTGAATTACAAGGATTTTTGGCATTGAGACCTTCTTTCAGGAACTTGGATTTTTCCAAAGG TCACCAGGGTATTACAGGGGACAAAGAAGGCCAGCAACGACGAATACGACAGCAACGCTTGATCTCTATA GGCAAATGGATTGCTGATAATCAGCCAAGGCTGATTCAGTGTGAAAATGAGGTAGGGAAATTGTTGTTTA TCACAGAAATCCCAGAATTAATACTGGAAGACCCCAGTGAAGCCAAAGAGAACCTCATTCTGCAAGAAAC ATCTGTGATAGAGTCGCTGGCTGCAGATGGGAGCCCAGGGCTAAAATCAGTGCTATCTACAAGCCGAAAT TTAAGCAACAACTGTGACACAGGAGAGAAGCCAGTGGTTACCTTCAAAGAAAACATTAAGACACGAGAAG TGAACAGAGACCAAGGAAGAAGTTTTCCTCCCAAAGAGGTGAGAAGGGACTATAGCAAAGGAATAACTGT AACTAAGAATGATGGAAAGAAGGACAACAACAAGAGGAAAACTGAAACCAAGAAATGCACCTTAGAAAAG TTACAGGAAACAGGAAAGCAGAATGTGGCAGTGCAGGTAAAATCCCAGACAGAACTAAGAAAGACTCCAG TGTCTGAAGCCAGAAAAACACCTGTAACTCAAACCCCAACTCAAGCAAGTAACTCCCAGTTCATCCCCAT TCATCACCCTGGAGCCTTCCCTCCTCTTCCCAGCAGGCCAGGGTTTCCGCCCCCAACATATGTTATCCCC CCGCCTGTGGCATTTTCTATGGGCTCAGGTTACACCTTCCCAGCTGGTGTTTCTGTCCCAGGAACCTTTC TTCAGCCTACAGCTCACTCTCCAGCAGGAAACCAGGTGCAAGCTGGGAAACAGTCCCACATTCCTTACAG CCAGCAACGGCCCTCTGGACCAGGGCCAATGAACCAGGGACCTCAACAATCACAGCCACCTTCCCAGCAA CCCCTTACATCTTTACCAGCTCAGCCAACAGCACAGTCTACAAGCCAGCTGCAGGTTCAAGCTCTAACTC AGCAACAACAATCCCCTACAAAAGCTGTGCCGGCTTTGGGGAAAAGCCCGCCTCACCACTCTGGATTCCA GCAGTATCAACAGGCAGATGCCTCCAAACAGCTGTGGAATCCCCCTCAGGTTCAAGGCCCATTAGGGAAA ATTATGCCTGTGAAACAGCCCTACTACCTTCAGACCCAAGACCCCATAAAACTGTTTGAGCCGTCATTGC AACCTCCTGTAATGCAGCAGCAGCCTCTAGAAAAAAAAATGAAGCCTTTTCCCATGGAGCCATATAACCA TAATCCCTCAGAAGTCAAGGTCCCAGAATTCTACTGGGATTCTTCCTACAGCATGGCTGATAACAGATCT GTAATGGCACAGCAAGCAAACATAGACCGCAGGGGCAAACGGTCACCAGGAGTCTTCCGTCCAGAGCAGG ATCCTGTACCCAGAATGCCGTTTGAGGACCCCAAGAGCTCCCCTCTGCTTCCTCCGGACCTGTTAAAGAG TCTGGCTGCCTTGGAGGAAGAGGAAGAGCTGATTTTTTCTAACCCTCCTGATCTTTACCCGGCTCTGCTG GGGCCTCTCGCCTCTCTTCCTGGACGAAGCCTTTTTAAATCCTTATTGGAGAAGCCCTCAGAGCTCATGT CACATTCATCCTCTTTCCTGTCCCTCACCGGATTCTCTCTCAATCAGGAAAGATACCCAAATAATAGTAT GTTCAATGAGGTATATGGGAAAAACCTGACATCCAGCTCCAAAGCAGAACTCAGTCCCTCAATGGCCCCC CAGGAAACATCTCTGTATTCCCTTTTTGAAGGGACTCCGTGGTCTCCATCACTTCCTGCCAGTTCAGATC ATTCAACACCAGCCAGCCAGTCTCCTCATTCCTCTAACCCAAGCAGCCTACCCAGCTCTCCTCCAACACA CAACCATAATTCTGTTCCATTCTCCAATTTTGGACCCATTGGGACTCCAGATAACAGGGATAGAAGGACT GCAGATCGGTGGAAAACTGATAAGCCAGCCATGGGTGGGTTTGGCATTGATTATCTCTCAGCAACGTCAT CCTCTGAGAGCAGTTGGCATCAGGCCAGCACTCCGAGTGGCACCTGGACAGGCCATGGCCCTTCCATGGA GGATTCCTCTGCTGTCCTCATGGAAAGCCTAAAGTCTATCTGGTCCAGTTCCATGATGCATCCTGGACCT TCTGCTCTGGAGCAGCTGTTAATGCAGCAGAAGCAGAAACAGCAACGGGGACAAGGCACCATGAACCCTC CACAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC230659 representing NM_001174061
Red=Cloning site Green=Tags(s) MLVTDLEYALDKKVEQDLWNHAFKNQITTLQGQAKNRANPNRSEVQANLSLFLEAASGFYTQLLQELCTV FNVDLPCRVKSSQLGIISNKQTHTSAIVKPQSSSCSYICQHCLVHLGDIARYRNQTSQAESYYRHAAQLV PSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNLQKALSKALESRDEVKTKWGVSDFIKA FIKFHGHVYLSKSLEKLSPLREKLEEQFKRLLFQKAFNSQQLVHVTVINLFQLHHLRDFSNETEQHTYSQ DEQLCWTQLLALFMSFLGILCKCPLQNESQEESYNAYPLPAVKVSMDWLRLRPRVFQEAVVDERQYIWPW LISLLNSFHPHEEDLSSISATPLPEEFELQGFLALRPSFRNLDFSKGHQGITGDKEGQQRRIRQQRLISI GKWIADNQPRLIQCENEVGKLLFITEIPELILEDPSEAKENLILQETSVIESLAADGSPGLKSVLSTSRN LSNNCDTGEKPVVTFKENIKTREVNRDQGRSFPPKEVRRDYSKGITVTKNDGKKDNNKRKTETKKCTLEK LQETGKQNVAVQVKSQTELRKTPVSEARKTPVTQTPTQASNSQFIPIHHPGAFPPLPSRPGFPPPTYVIP PPVAFSMGSGYTFPAGVSVPGTFLQPTAHSPAGNQVQAGKQSHIPYSQQRPSGPGPMNQGPQQSQPPSQQ PLTSLPAQPTAQSTSQLQVQALTQQQQSPTKAVPALGKSPPHHSGFQQYQQADASKQLWNPPQVQGPLGK IMPVKQPYYLQTQDPIKLFEPSLQPPVMQQQPLEKKMKPFPMEPYNHNPSEVKVPEFYWDSSYSMADNRS VMAQQANIDRRGKRSPGVFRPEQDPVPRMPFEDPKSSPLLPPDLLKSLAALEEEEELIFSNPPDLYPALL GPLASLPGRSLFKSLLEKPSELMSHSSSFLSLTGFSLNQERYPNNSMFNEVYGKNLTSSSKAELSPSMAP QETSLYSLFEGTPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHNSVPFSNFGPIGTPDNRDRRT ADRWKTDKPAMGGFGIDYLSATSSSESSWHQASTPSGTWTGHGPSMEDSSAVLMESLKSIWSSSMMHPGP SALEQLLMQQKQKQQRGQGTMNPPH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001174061 |
| ORF Size | 3435 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001174061.2 |
| RefSeq ORF | 3438 bp |
| Locus ID | 9887 |
| UniProt ID | Q92540 |
| MW | 128.3 kDa |
| Gene Summary | This gene encodes a protein that is essential for nonsense-mediated mRNA decay (NMD); a process whereby transcripts with premature termination codons are targeted for rapid degradation by a mRNA decay complex. The mRNA decay complex consists, in part, of this protein along with proteins SMG5 and UPF1. The N-terminal domain of this protein is thought to mediate its association with SMG5 or UPF1 while the C-terminal domain interacts with the mRNA decay complex. This protein may therefore couple changes in UPF1 phosphorylation state to the degradation of NMD-candidate transcripts. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Aug 2011] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC230659L3 | Lenti-ORF clone of SMG7 (Myc-DDK-tagged)-Human Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (SMG7), transcript variant 5 |
CNY 10,260.00 |
|
| RC230659L4 | Lenti-ORF clone of SMG7 (mGFP-tagged)-Human Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (SMG7), transcript variant 5 |
CNY 10,260.00 |
|
| RG230659 | SMG7 (tGFP-tagged) - Human Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (SMG7), transcript variant 5 |
CNY 9,220.00 |
|
| SC329297 | SMG7 (untagged)-Human Smg-7 homolog nonsense mediated mRNA decay factor (C. elegans) (SMG7) transcript variant 5 |
CNY 22,040.00 |
