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SCUBE2 (NM_001170690) Human Tagged ORF Clone
CAT＃: RC230505
SCUBE2 (Myc-DDK-tagged)-Human signal peptide, CUB domain, EGF-like 2 (SCUBE2), transcript variant 2
ORF Plasmid: tGFP
"NM_001170690" in other vectors (4)
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CNY 5,912.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CEGB1; CEGF1; CEGP1; scube/You |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC230505 representing NM_001170690
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGGTCGCGGGCCGCAACCGTCCCGGGGCGGCCTGGGCGGTGCTGCTGCTGCTGCTGCTGCTGCCGC CACTGCTGCTGCTGGCGGGGGCCGTCCCGCCGGGTCGGGGCCGTGCCGCGGGGCCGCAGGAGGATGTAGA TGAGTGTGCCCAAGGGCTAGATGACTGCCATGCCGACGCCCTGTGTCAGAACACACCCACCTCCTACAAG TGCTCCTGCAAGCCTGGCTACCAAGGGGAAGGCAGGCAGTGTGAGGACATCGATGAATGTGGAAATGAGC TCAATGGAGGCTGTGTCCATGACTGTTTGAATATTCCAGGCAATTATCGTTGCACTTGTTTTGATGGCTT CATGTTGGCTCATGACGGTCATAATTGTCTTGATGTGGACGAGTGCCTGGAGAACAATGGCGGCTGCCAG CATACCTGTGTCAACGTCATGGGGAGCTATGAGTGCTGCTGCAAGGAGGGGTTTTTCCTGAGTGACAATC AGCACACCTGCATTCACCGCTCGGAAGAGGGCCTGAGCTGCATGAATAAGGATCACGGCTGTAGTCACAT CTGCAAGGAGGCCCCAAGGGGCAGCGTCGCCTGTGAGTGCAGGCCTGGTTTTGAGCTGGCCAAGAACCAG AGAGACTGCATCTTGACCTGTAACCATGGGAACGGTGGGTGCCAGCACTCCTGTGACGATACAGCCGATG GCCCAGAGTGCAGCTGCCATCCACAGTACAAGATGCACACAGATGGGAGGAGCTGCCTTGAGCGAGAGGA CACTGTCCTGGAGGTGACAGAGAGCAACACCACATCAGTGGTGGATGGGGATAAACGGGTGAAACGGCGG CTGCTCATGGAAACGTGTGCTGTCAACAATGGAGGCTGTGACCGCACCTGTAAGGATACTTCGACAGGTG TCCACTGCAGTTGTCCTGTTGGATTCACTCTCCAGTTGGATGGGAAGACATGTAAAGATATTGATGAGTG CCAGACCCGCAATGGAGGTTGTGATCATTTCTGCAAAAACATCGTGGGCAGTTTTGACTGCGGCTGCAAG AAAGGATTTAAATTATTAACAGATGAGAAGTCTTGCCAAGATGTGGATGAGTGCTCTTTGGATAGGACCT GTGACCACAGCTGCATCAACCACCCTGGCACATTTGCTTGTGCTTGCAACCGAGGGTACACCCTGTATGG CTTCACCCACTGTGGAGACACCAATGAGTGCAGCATCAACAACGGAGGCTGTCAGCAGGTCTGTGTGAAC ACAGTGGGCAGCTATGAATGCCAGTGCCACCCTGGGTACAAGCTCCACTGGAATAAAAAAGACTGTGTGG CTTCTTGTGACCTGAGCTGCATCGTAAAGCGAACCGAGAAGCGGCTCCGTAAAGCCATCCGCACGCTCAG AAAGGCCGTCCACAGGGAGCAGTTTCACCTCCAGCTCTCAGGCATGAACCTCGACGTGGCTAAAAAGCCT CCCAGAACATCTGAACGCCAGGCAGAGTCCTGTGGAGTGGGCCAGGGTCATGCAGAAAACCAATGTGTCA GTTGCAGGGCTGGGACCTATTATGATGGAGCACGAGAACGCTGCATTTTATGTCCAAATGGAACCTTCCA AAATGAGGAAGGACAAATGACTTGTGAACCATGCCCAAGACCAGGAAATTCTGGGGCCCTGAAGACCCCA GAAGCTTGGAATATGTCTGAATGTGGAGGTCTGTGTCAACCTGGTGAATATTCTGCAGATGGCTTTGCAC CTTGCCAGCTCTGTGCCCTGGGCACGTTCCAGCCTGAAGCTGGTCGAACTTCCTGCTTCCCCTGTGGAGG AGGCCTTGCCACCAAACATCAGGGAGCTACTTCCTTTCAGGACTGTGAAACCAGAGTTCAATGTTCACCT GGACATTTCTACAACACCACCACTCACCGATGTATTCGTTGCCCAGTGGGAACATACCAGCCTGAATTTG GAAAAAATAATTGTGTTTCTTGCCCAGGAAATACTACGACTGACTTTGATGGCTCCACAAACATAACCCA GTGTAAAACTTCATCCAATTCTGTGACAACATATGAAACCTGCCAGACCTACGAACGCCCCATCGCCTTC ACCTCCAGGTCAAAGAAGCTGTGGATTCAGTTCAAGTCCAATGAAGGGAACAGCGCTAGAGGGTTCCAGG TCCCATACGTGACATATGATGAGGACTACCAGGAACTCATTGAAGACATAGTTCGAGATGGCAGGCTCTA TGCATCTGAGAACCATCAGGAAATACTTAAGGATAAGAAACTTATCAAGGCTCTGTTTGATGTCCTGGCC CATCCCCAGAACTATTTCAAGTACACAGCCCAGGAGTCCCGAGAGATGTTTCCAAGATCGTTCATCCGAT TGCTACGTTCCAAAGTGTCCAGGTTTTTGAGACCTTACAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC230505 representing NM_001170690
Red=Cloning site Green=Tags(s) MGVAGRNRPGAAWAVLLLLLLLPPLLLLAGAVPPGRGRAAGPQEDVDECAQGLDDCHADALCQNTPTSYK CSCKPGYQGEGRQCEDIDECGNELNGGCVHDCLNIPGNYRCTCFDGFMLAHDGHNCLDVDECLENNGGCQ HTCVNVMGSYECCCKEGFFLSDNQHTCIHRSEEGLSCMNKDHGCSHICKEAPRGSVACECRPGFELAKNQ RDCILTCNHGNGGCQHSCDDTADGPECSCHPQYKMHTDGRSCLEREDTVLEVTESNTTSVVDGDKRVKRR LLMETCAVNNGGCDRTCKDTSTGVHCSCPVGFTLQLDGKTCKDIDECQTRNGGCDHFCKNIVGSFDCGCK KGFKLLTDEKSCQDVDECSLDRTCDHSCINHPGTFACACNRGYTLYGFTHCGDTNECSINNGGCQQVCVN TVGSYECQCHPGYKLHWNKKDCVASCDLSCIVKRTEKRLRKAIRTLRKAVHREQFHLQLSGMNLDVAKKP PRTSERQAESCGVGQGHAENQCVSCRAGTYYDGARERCILCPNGTFQNEEGQMTCEPCPRPGNSGALKTP EAWNMSECGGLCQPGEYSADGFAPCQLCALGTFQPEAGRTSCFPCGGGLATKHQGATSFQDCETRVQCSP GHFYNTTTHRCIRCPVGTYQPEFGKNNCVSCPGNTTTDFDGSTNITQCKTSSNSVTTYETCQTYERPIAF TSRSKKLWIQFKSNEGNSARGFQVPYVTYDEDYQELIEDIVRDGRLYASENHQEILKDKKLIKALFDVLA HPQNYFKYTAQESREMFPRSFIRLLRSKVSRFLRPYK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001170690 |
| ORF Size | 2421 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001170690.2 |
| RefSeq Size | 4058 bp |
| RefSeq ORF | 2424 bp |
| Locus ID | 57758 |
| UniProt ID | Q9NQ36 |
| Protein Families | Transmembrane |
| MW | 88.9 kDa |
| Gene Summary | Lipid-binding protein required for SHH long-range signaling by binding to the dually lipid-modified SHH (ShhNp) and by promoting ShhNp mobilization, solubilization and release from the cell membrane (PubMed:22902404, PubMed:22677548). Acts by enhancing the proteolytic processing (shedding) of the lipid-modified N- and C- terminal of ShhNp at the cell surface (PubMed:24522195). Synergizes with DISP1 to increase SHH secretion (PubMed:22902404). Probable cell surface coreceptor for VEGFR2 involved in VEGFR2-mediated angiogenesis (PubMed:27834687).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC230505L3 | Lenti-ORF clone of SCUBE2 (Myc-DDK-tagged)-Human signal peptide, CUB domain, EGF-like 2 (SCUBE2), transcript variant 2 |
CNY 5,890.00 |
|
| RC230505L4 | Lenti-ORF clone of SCUBE2 (mGFP-tagged)-Human signal peptide, CUB domain, EGF-like 2 (SCUBE2), transcript variant 2 |
CNY 5,890.00 |
|
| RG230505 | SCUBE2 (tGFP-tagged) - Human signal peptide, CUB domain, EGF-like 2 (SCUBE2), transcript variant 2 |
CNY 4,370.00 |
|
| SC329143 | SCUBE2 (untagged)-Human signal peptide CUB domain EGF-like 2 (SCUBE2) transcript variant 2 |
CNY 7,220.00 |
