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KLHL13 (NM_001168301) Human Tagged ORF Clone
CAT＃: RC230380
KLHL13 (Myc-DDK-tagged)-Human kelch-like 13 (Drosophila) (KLHL13), transcript variant 4
ORF Plasmid: tGFP
"NM_001168301" in other vectors (4)
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CNY 4,760.00
CNY 7,505.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | BKLHD2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC230380 representing NM_001168301
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATCATCTGCATAGAGGGGAATTGGTTGCTGCAATACTTCGTAACCGATCTCTCGTGGAAGAGGAAG ACCAACACATGAAATTGTCCCTTGGAGGCAGCGAAATGGGCCTCTCATCCCATTTGCAGTCTTCCAAGGC AGGACCTACACGCATCTTTACCAGCAATACCCACAGTTCTGTGGTGTTACAGGGCTTTGACCAGCTTCGA CTTGAAGGATTGCTTTGTGATGTGACCCTGATGCCAGGTGACACAGATGATGCTTTCCCTGTGCATAGAG TCATGATGGCATCTGCTAGTGATTACTTCAAGGCTATGTTCACAGGTGGAATGAAAGAACAAGATTTAAT GTGCATTAAACTTCATGGTGTGAGCAAAGTCGGTCTAAGGAAAATTATTGATTTCATTTATACTGCAAAG CTTTCTCTTAATATGGACAACCTTCAAGACACGCTGGAAGCTGCCAGTTTCCTACAGATTCTGCCAGTTT TGGACTTCTGTAAAGTGTTTCTCATATCTGGGGTCACTTTAGACAACTGTGTTGAAGTTGGACGGATTGC CAACACCTACAATCTAACCGAAGTGGATAAATACGTTAACAGTTTCGTCTTGAAGAATTTTCCTGCATTG CTGAGCACAGGGGAGTTCTTGAAACTCCCTTTTGAGCGTCTTGCCTTCGTGCTTTCCAGTAATAGCCTTA AGCACTGTACTGAACTTGAGCTCTTTAAGGCTACCTGTCGTTGGCTTCGCCTGGAAGAGCCTCGGATGGA CTTTGCTGCAAAATTAATGAAGAACATACGATTTCCACTGATGACACCACAGGAGCTCATTAATTACGTG CAAACGGTGGATTTCATGAGAACTGACAATACTTGTGTGAATTTGCTTTTGGAAGCCAGCAATTACCAAA TGATGCCATATATGCAGCCAGTTATGCAGTCAGACAGGACTGCCATTAGGTCTGACACCACTCACTTGGT TACACTAGGAGGAGTGCTGAGGCAGCAGCTGGTTGTCAGTAAGGAATTGCGCATGTATGATGAAAAGGCC CATGAGTGGAAATCGTTAGCCCCCATGGATGCCCCAAGGTACCAGCATGGCATCGCCGTCATTGGAAATT TTCTCTATGTGGTTGGCGGACAGAGTAATTATGATACAAAAGGAAAAACGGCAGTTGATACAGTCTTCAG ATTTGATCCTCGATACAATAAATGGATGCAAGTTGCATCTTTAAATGAAAAGCGCACCTTCTTCCACCTA AGTGCCCTCAAAGGATATCTGTATGCAGTTGGTGGGCGAAATGCAGCAGGTGAACTGCCCACAGTAGAAT GTTACAATCCAAGAACAAATGAATGGACCTATGTTGCCAAAATGAGTGAGCCCCACTATGGCCATGCTGG AACTGTGTATGGAGGAGTGATGTATATTTCAGGAGGAATTACTCATGATACTTTCCAAAAGGAGCTCATG TGCTTTGACCCTGATACTGACAAATGGATCCAGAAGGCGCCAATGACCACTGTCAGAGGTCTGCATTGCA TGTGTACAGTGGGAGAAAGGCTCTATGTCATTGGTGGCAATCACTTCAGAGGAACAAGTGATTATGATGA TGTCCTAAGCTGTGAATACTATTCACCTATCCTTGACCAGTGGACCCCAATTGCTGCCATGTTAAGAGGG CAGAGTGATGTTGGGGTCGCTGTCTTCGAAAATAAAATCTATGTGGTTGGGGGGTATTCTTGGAATAATC GTTGTATGGTAGAGATAGTGCAGAAATATGATCCAGATAAAGATGAATGGCATAAGGTTTTTGATCTGCC AGAATCCCTTGGTGGCATTCGTGCTTGCACACTCACAGTTTTTCCACCAGAAGAAACCACACCATCACCT TCTAGAGAGTCCCCTCTTTCTGCACCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC230380 representing NM_001168301
Red=Cloning site Green=Tags(s) MDHLHRGELVAAILRNRSLVEEEDQHMKLSLGGSEMGLSSHLQSSKAGPTRIFTSNTHSSVVLQGFDQLR LEGLLCDVTLMPGDTDDAFPVHRVMMASASDYFKAMFTGGMKEQDLMCIKLHGVSKVGLRKIIDFIYTAK LSLNMDNLQDTLEAASFLQILPVLDFCKVFLISGVTLDNCVEVGRIANTYNLTEVDKYVNSFVLKNFPAL LSTGEFLKLPFERLAFVLSSNSLKHCTELELFKATCRWLRLEEPRMDFAAKLMKNIRFPLMTPQELINYV QTVDFMRTDNTCVNLLLEASNYQMMPYMQPVMQSDRTAIRSDTTHLVTLGGVLRQQLVVSKELRMYDEKA HEWKSLAPMDAPRYQHGIAVIGNFLYVVGGQSNYDTKGKTAVDTVFRFDPRYNKWMQVASLNEKRTFFHL SALKGYLYAVGGRNAAGELPTVECYNPRTNEWTYVAKMSEPHYGHAGTVYGGVMYISGGITHDTFQKELM CFDPDTDKWIQKAPMTTVRGLHCMCTVGERLYVIGGNHFRGTSDYDDVLSCEYYSPILDQWTPIAAMLRG QSDVGVAVFENKIYVVGGYSWNNRCMVEIVQKYDPDKDEWHKVFDLPESLGGIRACTLTVFPPEETTPSP SRESPLSAP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001168301 |
| ORF Size | 1917 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001168301.1, NP_001161773.1 |
| RefSeq ORF | 1920 bp |
| Locus ID | 90293 |
| UniProt ID | Q9P2N7 |
| Protein Pathways | Ubiquitin mediated proteolysis |
| MW | 72.6 kDa |
| Gene Summary | This gene encodes a BTB and kelch domain containing protein and belongs to the kelch repeat domain containing superfamily of proteins. The encoded protein functions as an adaptor protein that complexes with Cullin 3 and other proteins to form the Cullin 3-based E3 ubiquitin-protein ligase complex. This complex is necessary for proper chromosome segregation and completion of cytokinesis. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC230380L3 | Lenti-ORF clone of KLHL13 (Myc-DDK-tagged)-Human kelch-like 13 (Drosophila) (KLHL13), transcript variant 4 |
CNY 5,890.00 |
|
| RC230380L4 | Lenti-ORF clone of KLHL13 (mGFP-tagged)-Human kelch-like 13 (Drosophila) (KLHL13), transcript variant 4 |
CNY 5,890.00 |
|
| RG230380 | KLHL13 (tGFP-tagged) - Human kelch-like 13 (Drosophila) (KLHL13), transcript variant 4 |
CNY 4,370.00 |
|
| SC329018 | KLHL13 (untagged)-Human kelch-like 13 (Drosophila) (KLHL13) transcript variant 4 |
CNY 10,260.00 |
