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SYT12 (NM_001177880) Human Tagged ORF Clone
CAT＃: RC230052
- TrueORF®
SYT12 (Myc-DDK-tagged)-Human synaptotagmin XII (SYT12), transcript variant 2
ORF Plasmid: tGFP
"NM_001177880" in other vectors (4)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | SYT11; sytXII |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC230052 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGTGGATGTGGCAGAATACCATCTGAGCGTCATCAAGAGCCCCCCTGGCTGGGAGGTGGGTGTCT ATGCTGCAGGGGCCCTGGCCCTGCTGGGAATCGCAGCTGTGAGCCTGTGGAAGCTCTGGACGTCGGGGAG CTTCCCCAGCCCCTCTCCGTTCCCCAATTACGACTACAGGTACCTTCAGCAGAAGTACGGCGAGAGCTGC GCAGAGGCCAGGGAGAAGAGAGTGCCTGCCTGGAATGCCCAGCGGGCCAGCACGCGGGGACCACCCAGCC GCAAAGGCAGTCTCAGCATTGAGGACACCTTTGAGAGCATCAGTGAACTGGGGCCTCTGGAGCTGATGGG CCGGGAGTTGGACCTGGCCCCCTATGGGACCCTCCGGAAGTCCCAGTCGGCCGACTCCCTGAACTCCATC TCCTCCGTGAGCAACACCTTTGGGCAGGACTTCACACTGGGCCAGGTGGAGGTGAGCATGGAGTACGACA CTGCCTCCCACACGCTGAACGTGGCGGTGATGCAGGGCAAGGACCTCCTGGAGCGGGAGGAGGCCAGCTT CGAGTCCTGCTTCATGCGCGTCAGCCTGCTGCCGGACGAGCAGATCGTGGGCATTTCTCGGATCCAGAGA AATGCCTACTCCATCTTCTTTGATGAGAAGTTCTCCATCCCCCTGGATCCCACAGCCCTGGAGGAGAAGA GCCTGCGGTTTTCTGTATTTGGCATCGATGAGGATGAGCGCAACGTCAGCACGGGGGTGGTGGAGCTGAA GCTTTCTGTGCTTGACCTCCCGCTGCAGCCCTTCAGTGGCTGGCTCTATTTACAGGACCAGAACAAGGCC GCCGATGCTGTGGGGGAGATCCTGCTCTCCCTCAGCTACCTCCCCACAGCCGAGCGCCTCACCGTGGTCG TGGTTAAGGCCAAGAACCTCATCTGGACCAACGACAAGACCACAGCGGACCCCTTCGTCAAGGTGTACCT GCTGCAGGATGGGAGGAAGATGAGCAAAAAGAAGACAGCCGTGAAGAGGGATGACCCCAACCCGGTGTTC AACGAAGCCATGATCTTCTCGGTGCCAGCCATTGTGCTCCAGGACCTGTCTCTCCGCGTGACGGTGGCTG AGAGCAGCAGCGACGGCCGTGGGGACAACGTGGGCCATGTCATCATTGGGCCGTCAGCCAGTGGCATGGG AACCACACATTGGAACCAGATGTTGGCCACGCTGCGCAGGCCCGTGTCCATGTGGCACGCTGTCCGGCGA AAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC230052 protein sequence
Red=Cloning site Green=Tags(s) MAVDVAEYHLSVIKSPPGWEVGVYAAGALALLGIAAVSLWKLWTSGSFPSPSPFPNYDYRYLQQKYGESC AEAREKRVPAWNAQRASTRGPPSRKGSLSIEDTFESISELGPLELMGRELDLAPYGTLRKSQSADSLNSI SSVSNTFGQDFTLGQVEVSMEYDTASHTLNVAVMQGKDLLEREEASFESCFMRVSLLPDEQIVGISRIQR NAYSIFFDEKFSIPLDPTALEEKSLRFSVFGIDEDERNVSTGVVELKLSVLDLPLQPFSGWLYLQDQNKA ADAVGEILLSLSYLPTAERLTVVVVKAKNLIWTNDKTTADPFVKVYLLQDGRKMSKKKTAVKRDDPNPVF NEAMIFSVPAIVLQDLSLRVTVAESSSDGRGDNVGHVIIGPSASGMGTTHWNQMLATLRRPVSMWHAVRR N TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001177880 |
| ORF Size | 1263 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001177880.2 |
| RefSeq Size | 3632 bp |
| RefSeq ORF | 1266 bp |
| Locus ID | 91683 |
| UniProt ID | Q8IV01 |
| Protein Families | Secreted Protein, Transmembrane |
| MW | 46.5 kDa |
| Gene Summary | This gene is a member of the synaptotagmin gene family and encodes a protein similar to other family members that mediate calcium-dependent regulation of membrane trafficking in synaptic transmission. Studies of the orthologous gene in rat have shown that the encoded protein selectively modulates spontaneous synaptic-vesicle exocytosis and may also be involved in regulating calcium independent secretion in nonneuronal cells. Alternative splicing results in multiple transcript variants. The gene has previously been referred to as synaptotagmin XI but has been renamed synaptotagmin XII to be standard with mouse and rat official nomenclature.[provided by RefSeq, Apr 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC230052L3 | Lenti-ORF clone of SYT12 (Myc-DDK-tagged)-Human synaptotagmin XII (SYT12), transcript variant 2 |
CNY 5,890.00 |
|
| RC230052L4 | Lenti-ORF clone of SYT12 (mGFP-tagged)-Human synaptotagmin XII (SYT12), transcript variant 2 |
CNY 5,890.00 |
|
| RG230052 | SYT12 (tGFP-tagged) - Human synaptotagmin XII (SYT12), transcript variant 2 |
CNY 4,370.00 |
|
| SC328690 | SYT12 (untagged)-Human synaptotagmin XII (SYT12) transcript variant 2 |
CNY 7,220.00 |
