Kir7.1 (KCNJ13) (NM_001172417) Human Tagged ORF Clone

CAT#: RC229820

  • TrueORF®

KCNJ13 (Myc-DDK-tagged)-Human potassium inwardly-rectifying channel, subfamily J, member 13 (KCNJ13), transcript variant 3

ORF Plasmid: DDK tGFP



  "NM_001172417" in other vectors (4)

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CNY 3,990.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


KCNJ13 Rabbit polyclonal Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms KIR1.4; KIR7.1; LCA16; SVD
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC229820 representing NM_001172417
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAATGGTGATCTGGAACTAGATCATGATGCCCCACCTGAAAACCACACTATCTGTGTCAAGTATATCA
CCAGTTTCACAGCTGCATTCTCCTTCTCCCTGGAGACACAACTCACAATTGGTTATGGTACCATGTTCCC
CAGTGGTGACTGTCCAAGTGCAATCGCCTTACTTGCCATACAAATGCTCCTAGGCCTCATGCTAGAGGCT
TTTATCACAGGTGCTTTTGTGGCGAAGATTGCCCGGCCAAAAAATCGAGCTTTTTCAATTCGCTTTACTG
ACACAGCAGTAGTAGCTCACATGGATGGCAAACCTAATCTTATCTTCCAAGTGGCCAACACCCGACCTAG
CCCTCTAACCAGTGTCCGGGTCTCAGCTGTACTCTATCAGGAAAGAGAAAATGGCAAACTCTACCAGACC
AGTGTGGATTTCCACCTTGATGGCATCAGTTCTGACGAATGTCCATTCTTCATCTTTCCACTAACGTACT
ATCACTCCATTACACCATCAAGTCCTCTGGCTACTCTGCTCCAGCATGAAAATCCTTCTCACTTTGAATT
AGTTGTATTCCTTTCAGCAATGCAGGAGGGCACTGGAGAAATATGCCAAAGGAGGACATCCTACCTACCG
TCTGAAATCATGTTACATCACTGTTTTGCATCTCTGTTGACCCGAGGTTCCAAAGGTGAATATCAAATCA
AGATGGAGAATTTTGACAAGACTGTCCCTGAATTTCCAACTCCTCTGGTTTCTAAAAGCCCAAACAGGAC
TGACCTGGATATCCACATCAATGGACAAAGCATTGACAATTTTCAGATCTCTGAAACAGGACTGACAGAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC229820 representing NM_001172417
Red=Cloning site Green=Tags(s)

MNGDLELDHDAPPENHTICVKYITSFTAAFSFSLETQLTIGYGTMFPSGDCPSAIALLAIQMLLGLMLEA
FITGAFVAKIARPKNRAFSIRFTDTAVVAHMDGKPNLIFQVANTRPSPLTSVRVSAVLYQERENGKLYQT
SVDFHLDGISSDECPFFIFPLTYYHSITPSSPLATLLQHENPSHFELVVFLSAMQEGTGEICQRRTSYLP
SEIMLHHCFASLLTRGSKGEYQIKMENFDKTVPEFPTPLVSKSPNRTDLDIHINGQSIDNFQISETGLTE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001172417
ORF Size 840 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001172417.1, NP_001165888.1
RefSeq Size 3376 bp
RefSeq ORF 843 bp
Locus ID 3769
UniProt ID O60928
Protein Families Druggable Genome, Ion Channels: Potassium, Transmembrane
MW 31.1 kDa
Gene Summary This gene encodes a member of the inwardly rectifying potassium channel family of proteins. Members of this family form ion channel pores that allow potassium ions to pass into a cell. The encoded protein belongs to a subfamily of low signal channel conductance proteins that have a low dependence on potassium concentration. Mutations in this gene are associated with snowflake vitreoretinal degeneration. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Feb 2010]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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