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SHANK2 (NM_012309) Human Tagged ORF Clone
CAT＃: RC229453
SHANK2 (Myc-DDK-tagged)-Human SH3 and multiple ankyrin repeat domains 2 (SHANK2), transcript variant 1
ORF Plasmid: tGFP
"NM_012309" in other vectors (3)
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CNY 12,432.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AUTS17; CORTBP1; CTTNBP1; ProSAP1; SHANK; SPANK-3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC229453 representing NM_012309
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGCGCAGCCCAACATCCAGCGAGGACGAGATGGCCCAGAGCTTCTCCGACTACTCCGTGGGGTCGG AGTCAGACAGCTCCAAAGAAGAGACCATCTATGACACGATCCGGGCCACTGCGGAGAAGCCGGGCGGTGC CAGGACGGAGGAGAGCCAGGGCAACACGCTGGTGATCCGCGTGGTCATCCATGACCTGCAGCAGACGAAA TGCATTCGATTTAACCCGGATGCCACAGTGTGGGTTGCAAAGCAGCGGATCCTGTGTACATTAACCCAGA GTTTGAAAGATGTCCTGAACTACGGCCTGTTCCAGCCGGCCAGCAATGGGCGTGACGGCAAGTTCCTGGA TGAGGAGCGGCTCCTGCGCGAGTACCCACAGCCCGTGGGTGAGGGCGTTCCTTCCCTGGAGTTTCGATAC AAGAAGCGGGTGTATAAACAAGCCAGTCTCGATGAGAAACAGTTGGCCAAGCTCCACACGAAGACCAATC TGAAGAAATGCATGGATCACATTCAGCATCGCTTGGTGGAGAAGATCACCAAGATGCTGGACCGAGGCCT GGATCCCAATTTCCACGACCCGGAGACCGGAGAGACCCCCCTGACCTTAGCCGCTCAGCTGGACGACTCT GTGGAGGTCATCAAAGCTCTCAAAAATGGTGGAGCTCACCTGGACTTCCGTGCCAAAGATGGGATGACCG CCCTACACAAAGCTGCCCGAGCGAGGAACCAAGTTGCCCTGAAGACCCTTTTAGAGCTTGGTGCATCCCC AGATTATAAAGACAGTTACGGCCTCACCCCGCTGTATCACACAGCCATCGTCGGAGGTGATCCCTACTGC TGCGAGCTTCTCCTGCACGAACACGCCACTGTGTGCTGCAAAGATGAGAACGGCTGGCACGAGATCCACC AGGCCTGCAGGTACGGGCACGTGCAGCACCTGGAGCACCTGCTGTTCTACGGGGCAGACATGAGTGCCCA GAATGCCTCGGGGAACACGGCCTTGCACATCTGCGCCCTCTACAACCAGGACAGCTGTGCAAGAGTGCTT CTGTTTCGAGGCGGAAATAAGGAGTTAAAAAACTACAACAGCCAGACTCCATTTCAGGTGGCCATAATAG CAGGCAACTTTGAGCTGGCAGAATACATCAAGAACCACAAGGAAACAGACATTGTGCCCTTCCGAGAGGC CCCGGCGTACTCCAACCGCCGGCGGCGGCCCCCCAACACGCTGGCCGCCCCCCGGGTCCTGCTGCGCTCC AACAGTGACAACAACCTCAATGCCAGCGCTCCCGACTGGGCCGTCTGCTCCACGGCCACCTCGCACCGCA GCCTGTCACCCCAGCTGCTGCAGCAGATGCCCAGCAAGCCCGAGGGGGCCGCGAAGACCATTGGGAGCTA CGTGCCCGGGCCCCGCAGCCGGTCCCCATCGCTCAACAGGCTGGGCGGCGCAGGCGAGGACGGCAAGAGG CCGCAGCCTCTCTGGCATGTCGGGTCGCCTTTTGCTCTTGGTGCCAACAAGGACTCACTCTCGGCCTTCG AGTACCCGGGGCCCAAGCGGAAGCTCTACAGTGCCGTGCCCGGGAGGCTCTTCGTCGCTGTCAAGCCATA CCAACCCCAAGTGGACGGCGAGATCCCCCTTCACCGCGGTGACAGGGTCAAAGTTCTGAGCATCGGTGAA GGGGGCTTCTGGGAAGGCAGCGCCCGCGGCCACATCGGATGGTTTCCGGCGGAGTGCGTGGAGGAGGTCC AGTGTAAGCCCAGGGACAGCCAGGCAGAAACCCGCGCTGACCGCAGCAAGAAGCTTTTCAGGCACTACAC CGTGGGCTCCTATGACAGCTTCGACACTTCCAGTGACTGCATTATTGAGGAGAAGACGGTGGTCCTGCAG AAAAAAGACAATGAGGGCTTTGGATTCGTGCTTCGAGGGGCCAAAGCTGACACACCCATTGAAGAATTCA CACCAACACCGGCTTTCCCAGCCCTACAGTACCTGGAGTCCGTGGATGAAGGTGGGGTGGCGTGGCAAGC CGGACTAAGGACCGGGGACTTCTTGATTGAGGTTAACAATGAGAATGTTGTCAAAGTCGGCCACAGGCAG GTGGTGAACATGATCCGGCAGGGAGGGAATCACCTGGTCCTTAAGGTGGTCACGGTGACCAGGAATCTGG ACCCCGACGACACCGCCAGGAAGAAAGCTCCCCCGCCTCCAAAGCGGGCACCGACCACAGCCCTCACCCT GCGCTCCAAGTCCATGACCTCGGAGCTGGAGGAGCTCGTGGATAAAGCCTCGGTCCGGAAGAAGAAGGAT AAACCCGAGGAGATAGTCCCGGCCTCCAAGCCCTCCCGCGCTGCTGAGAACATGGCTGTGGAACCGAGGG TGGCGACCATCAAGCAGCGGCCCAGCAGCCGGTGCTTCCCGGCGGGCTCAGACATGAACTCTGTGTACGA ACGCCAAGGAATCGCCGTGATGACGCCCACTGTTCCTGGGAGCCCAAAAGCCCCGTTTCTGGGCATCCCT CGAGGTACGATGCGAAGGCAGAAATCAATAGACAGCAGAATCTTTCTATCAGGAATAACAGAGGAAGAGC GGCAGTTTCTGGCTCCTCCAATGCTGAAGTTCACCAGAAGCCTGTCCATGCCGGACACCTCTGAGGACAT CCCCCCTCCACCGCAGTCTGTGCCCCCGTCCCCACCACCACCTTCCCCAACCACTTACAACTGCCCCAAG TCCCCAACTCCAAGAGTCTACGGGACGATTAAGCCTGCGTTCAATCAGAATTCTGCCGCCAAGGTGTCCC CCGCCACCAGGTCCGACACCGTGGCCACCATGATGAGGGAGAAGGGGATGTACTTCAGGAGAGAGCTGGA CCGCTACTCCTTGGACTCTGAAGACCTCTACAGTCGGAATGCCGGCCCGCAAGCCAACTTCCGCAACAAG AGAGGCCAGATGCCAGAAAACCCATACTCAGAGGTGGGGAAGATCGCCAGCAAAGCCGTCTACGTCCCCG CCAAGCCCGCCAGGCGGAAGGGGATGCTGGTGAAGCAGTCCAACGTGGAGGACAGCCCCGAGAAGACGTG CTCCATCCCTATCCCGACCATCATCGTGAAGGAGCCGTCCACCAGCAGCAGCGGCAAGAGCAGCCAGGGC AGCAGCATGGAGATCGACCCCCAGGCCCCGGAGCCACCGAGCCAGCTGCGGCCTGACGAAAGCCTGACCG TCAGCAGCCCCTTTGCCGCCGCCATCGCCGGAGCCGTCCGCGACCGTGAGAAGCGGCTGGAAGCCAGGAG GAACTCCCCGGCCTTCCTCTCCACAGACCTGGGGGATGAGGATGTGGGCCTGGGGCCACCCGCCCCCAGG ACGCGGCCCTCCATGTTCCCCGAGGAGGGGGATTTTGCTGACGAGGACAGCGCTGAGCAGCTGTCATCCC CCATGCCGAGTGCCACGCCCAGGGAGCCCGAAAACCATTTCGTGGGTGGCGCCGAGGCCAGTGCTCCGGG TGAGGCTGGGAGGCCGCTGAATTCCACGTCCAAAGCCCAGGGGCCCGAGAGCAGCCCAGCAGTGCCCTCC GCGAGCAGCGGCACAGCCGGCCCCGGGAATTATGTCCACCCACTCACAGGGCGGCTGCTTGATCCCAGCT CCCCGCTGGCCCTGGCACTCTCCGCAAGGGACCGAGCCATGAAGGAGTCTCAACAGGGACCCAAAGGGGA GGCCCCCAAGGCCGACCTCAACAAACCTCTTTACATTGATACCAAAATGCGGCCCAGCCTGGATGCCGGC TTCCCTACGGTCACCAGGCAGAACACCCGGGGACCCCTGAGGCGGCAGGAGACGGAGAACAAGTACGAGA CCGACCTGGGCCGAGACCGGAAAGGCGATGACAAGAAGAACATGCTGATCGACATCATGGACACGTCCCA GCAGAAGTCGGCTGGCCTGCTGATGGTGCACACCGTGGACGCCACTAAGCTGGACAACGCCCTGCAGGAA GAGGACGAGAAGGCAGAGGTGGAGATGAAGCCAGACAGCTCGCCGTCCGAGGTGCCAGAAGGTGTTTCCG AAACCGAAGGTGCTTTACAGATCTCCGCTGCCCCCGAGCCCACCACCGTGCCCGGCAGAACCATCGTCGC GGTGGGCTCCATGGAAGAGGCGGTGATTTTGCCATTCCGCATCCCTCCTCCCCCTCTGGCATCCGTGGAC TTGGATGAGGATTTTATTTTTACAGAGCCATTGCCTCCTCCCCTGGAATTTGCAAATAGTTTTGATATCC CCGATGACCGGGCAGCTTCTGTCCCGGCTCTCTCAGACTTAGTGAAGCAGAAGAAAAGCGACACCCCTCA GTCCCCTTCGTTGAACTCCAGCCAACCAACCAACTCTGCAGACAGCAAGAAGCCAGCCAGTCTTTCAAAC TGTCTGCCTGCCTCATTCCTGCCACCCCCTGAAAGCTTTGACGCCGTCGCCGACTCTGGGATCGAGGAGG TGGACAGCCGGAGTAGCAGCGACCACCACCTCGAGACGACCAGCACTATCTCCACCGTGTCTAGCATCTC CACCCTGTCTTCCGAAGGTGGAGAGAATGTGGACACCTGCACAGTCTATGCAGATGGGCAAGCATTTATG GTTGACAAACCCCCAGTACCTCCTAAGCCAAAAATGAAGCCCATCATTCACAAAAGCAATGCACTTTATC AAGACGCGCTCGTGGAAGAAGATGTAGATAGCTTTGTTATCCCCCCGCCCGCTCCCCCGCCCCCGCCGGG CAGTGCCCAGCCTGGGATGGCCAAGGTTCTCCAGCCAAGGACCTCCAAGTTGTGGGGCGACGTCACAGAG ATCAAAAGCCCGATTCTCTCAGGCCCAAAGGCAAACGTTATTAGTGAATTGAACTCTATCCTACAGCAAA TGAACCGAGAGAAATTGGCAAAGCCGGGGGAAGGACTGGATTCACCAATGGGAGCCAAGTCCGCCAGCCT CGCTCCAAGAAGCCCGGAGATCATGAGCACCATCTCAGGTACACGGAGCACGACGGTCACCTTCACTGTT CGCCCCGGCACCTCCCAGCCCATCACCCTGCAGAGCCGGCCCCCCGACTATGAAAGCAGGACCTCAGGAA CAAGACGTGCCCCAAGCCCTGTGGTCTCGCCAACAGAGATGAACAAAGAGACCCTGCCCGCCCCCCTGTC TGCTGCCACCGCCTCTCCTTCTCCCGCTCTCTCAGATGTCTTTAGCCTTCCAAGCCAGCCCCCTTCTGGG GATCTATTTGGCTTGAACCCAGCGGGACGCAGTAGGTCGCCATCCCCCTCGATACTGCAACAGCCAATCT CAAATAAGCCTTTTACAACTAAACCTGTCCACCTGTGGACTAAACCAGATGTGGCCGATTGGCTGGAAAG TCTAAACTTGGGTGAACATAAAGAGGCCTTCATGGACAATGAGATCGATGGCAGTCACTTACCAAACCTG CAGAAGGAGGACCTCATCGATCTTGGGGTAACTCGAGTCGGGCACAGAATGAACATAGAAAGGGCTTTGA AACAGCTGCTGGACAGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC229453 representing NM_012309
Red=Cloning site Green=Tags(s) MPRSPTSSEDEMAQSFSDYSVGSESDSSKEETIYDTIRATAEKPGGARTEESQGNTLVIRVVIHDLQQTK CIRFNPDATVWVAKQRILCTLTQSLKDVLNYGLFQPASNGRDGKFLDEERLLREYPQPVGEGVPSLEFRY KKRVYKQASLDEKQLAKLHTKTNLKKCMDHIQHRLVEKITKMLDRGLDPNFHDPETGETPLTLAAQLDDS VEVIKALKNGGAHLDFRAKDGMTALHKAARARNQVALKTLLELGASPDYKDSYGLTPLYHTAIVGGDPYC CELLLHEHATVCCKDENGWHEIHQACRYGHVQHLEHLLFYGADMSAQNASGNTALHICALYNQDSCARVL LFRGGNKELKNYNSQTPFQVAIIAGNFELAEYIKNHKETDIVPFREAPAYSNRRRRPPNTLAAPRVLLRS NSDNNLNASAPDWAVCSTATSHRSLSPQLLQQMPSKPEGAAKTIGSYVPGPRSRSPSLNRLGGAGEDGKR PQPLWHVGSPFALGANKDSLSAFEYPGPKRKLYSAVPGRLFVAVKPYQPQVDGEIPLHRGDRVKVLSIGE GGFWEGSARGHIGWFPAECVEEVQCKPRDSQAETRADRSKKLFRHYTVGSYDSFDTSSDCIIEEKTVVLQ KKDNEGFGFVLRGAKADTPIEEFTPTPAFPALQYLESVDEGGVAWQAGLRTGDFLIEVNNENVVKVGHRQ VVNMIRQGGNHLVLKVVTVTRNLDPDDTARKKAPPPPKRAPTTALTLRSKSMTSELEELVDKASVRKKKD KPEEIVPASKPSRAAENMAVEPRVATIKQRPSSRCFPAGSDMNSVYERQGIAVMTPTVPGSPKAPFLGIP RGTMRRQKSIDSRIFLSGITEEERQFLAPPMLKFTRSLSMPDTSEDIPPPPQSVPPSPPPPSPTTYNCPK SPTPRVYGTIKPAFNQNSAAKVSPATRSDTVATMMREKGMYFRRELDRYSLDSEDLYSRNAGPQANFRNK RGQMPENPYSEVGKIASKAVYVPAKPARRKGMLVKQSNVEDSPEKTCSIPIPTIIVKEPSTSSSGKSSQG SSMEIDPQAPEPPSQLRPDESLTVSSPFAAAIAGAVRDREKRLEARRNSPAFLSTDLGDEDVGLGPPAPR TRPSMFPEEGDFADEDSAEQLSSPMPSATPREPENHFVGGAEASAPGEAGRPLNSTSKAQGPESSPAVPS ASSGTAGPGNYVHPLTGRLLDPSSPLALALSARDRAMKESQQGPKGEAPKADLNKPLYIDTKMRPSLDAG FPTVTRQNTRGPLRRQETENKYETDLGRDRKGDDKKNMLIDIMDTSQQKSAGLLMVHTVDATKLDNALQE EDEKAEVEMKPDSSPSEVPEGVSETEGALQISAAPEPTTVPGRTIVAVGSMEEAVILPFRIPPPPLASVD LDEDFIFTEPLPPPLEFANSFDIPDDRAASVPALSDLVKQKKSDTPQSPSLNSSQPTNSADSKKPASLSN CLPASFLPPPESFDAVADSGIEEVDSRSSSDHHLETTSTISTVSSISTLSSEGGENVDTCTVYADGQAFM VDKPPVPPKPKMKPIIHKSNALYQDALVEEDVDSFVIPPPAPPPPPGSAQPGMAKVLQPRTSKLWGDVTE IKSPILSGPKANVISELNSILQQMNREKLAKPGEGLDSPMGAKSASLAPRSPEIMSTISGTRSTTVTFTV RPGTSQPITLQSRPPDYESRTSGTRRAPSPVVSPTEMNKETLPAPLSAATASPSPALSDVFSLPSQPPSG DLFGLNPAGRSRSPSPSILQQPISNKPFTTKPVHLWTKPDVADWLESLNLGEHKEAFMDNEIDGSHLPNL QKEDLIDLGVTRVGHRMNIERALKQLLDR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_012309 |
| ORF Size | 5547 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_012309.5 |
| RefSeq ORF | 5550 bp |
| Locus ID | 22941 |
| UniProt ID | Q9UPX8 |
| MW | 201.1 kDa |
| Gene Summary | This gene encodes a protein that is a member of the Shank family of synaptic proteins that may function as molecular scaffolds in the postsynaptic density of excitatory synapses. Shank proteins contain multiple domains for protein-protein interaction, including ankyrin repeats, and an SH3 domain. This particular family member contains a PDZ domain, a consensus sequence for cortactin SH3 domain-binding peptides and a sterile alpha motif. The alternative splicing demonstrated in Shank genes has been suggested as a mechanism for regulating the molecular structure of Shank and the spectrum of Shank-interacting proteins in the postsynaptic densities of the adult and developing brain. Alterations in the encoded protein may be associated with susceptibility to autism spectrum disorder. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
USP8 Deubiquitinates SHANK3 to Control Synapse Density and SHANK3 Activity-dependent Protein Levels
,Kerrisk Campbell, M;Sheng, M;,
J. Neurosci.
,PubMed ID 29735556
[SHANK2]
|
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RG229453 | SHANK2 (tGFP-tagged) - Human SH3 and multiple ankyrin repeat domains 2 (SHANK2), transcript variant 1 |
CNY 20,240.00 |
|
| SC303955 | SHANK2 (untagged)-Human SH3 and multiple ankyrin repeat domains 2 (SHANK2), transcript variant 1 |
CNY 35,630.00 |
|
| SC328088 | SHANK2 (untagged)-Human SH3 and multiple ankyrin repeat domains 2 (SHANK2) transcript variant 1 |
CNY 35,630.00 |
