PEG3 (NM_001146185) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	PW1; ZKSCAN22; ZNF904; ZSCAN24
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC229077 representing NM_001146185 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTACCAACCAGAAGACGACAACAACAGTGACGTGACCAGCGACGACGACATGACCCGGAACAGAAGAG AGTCCTCACCACCTCACTCAGTCCATTCTTTCAGTGACCGGGACTGGGACCGGAGGGGCAGAAGCAGAGA CATGGAGCCACGAGACCGCTGGTCCCACACCAGGAACCCAAGAAGCAGGATGCCTCCGCGGGATCTTTCC CTTCCTGTGGTGGCGAAAACAAGCTTTGAAATGGACAGAGAGGACGACAGGGACTCCAGGGCTTATGAGT CCCGATCTCAGGATGCTGAATCATACCAAAATGTGGTGGACCTCGCTGAGGACAGGAAACCTCACAACAC AATCCAGGACAACATGGAAAACTACAGGAAGCTGCTCTCCCTCGTGCAGCTTGCTGAAGACGATGGCCAC TCCCACATGACGCAGGGCCACTCATCAAGATCCAAGAGAAGTGCCTACCCAAGCACCAGTCGAGGTCTAA AAACTATGCCTGAAGCCAAAAAATCAACCCACCGGCGGGGGATTTGTGAAGATGAATCTTCCCACGGAGT GATAATGGAAAAATTCATCAAGGATGTGTCACGCAGTTCCAAATCGGGAAGAGCAAGGGAGTCAAGCGAC CGGTCACAGAGATTCCCCAGAATGTCAGATGATAACTGGAAGGACATTTCATTGAACAAGAGGGAGTCAG TGATCCAGCAGCGGGTTTATGAAGGGAATGCATTTAGGGGAGGCTTTAGGTTTAATTCAACCCTTGTTTC CAGAAAGAGAGTTCTTGAAAGAAAGAGGCGCTATCATTTTGACACAGATGGGAAGGGCTCGATTCACGAT CAAAAAGGCTGTCCCAGGAAGAAGCCCTTTGAATGTGGTAGTGAGATGAGAAAAGCCATGAGCGTGAGCA GCCTGAGCAGCCTCAGCTCCCCCTCCTTTACCGAGTCACAGCCAATTGATTTTGGGGCAATGCCATATGT ATGTGATGAGTGTGGGAGGTCGTTCAGTGTCATCTCAGAATTTGTTGAGCACCAGATCATGCATACTAGA GAGAACCTCTATGAGTATGGTGAGTCCTTTATCCACAGTGTGGCTGTCAGTGAAGTTCAGAAAAGTCAGG TTGGAGGGAAACGTTTTGAATGTAAGGACTGTGGAGAGACCTTCAATAAGAGTGCCGCCTTGGCTGAACA TCGGAAGATTCATGCTAGAGGTTATCTTGTGGAATGTAAGAATCAGGAATGTGAGGAAGCCTTCATGCCT AGCCCCACCTTTAGTGAGCTTCAGAAAATATATGGCAAAGACAAATTCTACGAGTGCAGGGTGTGTAAGG AAACCTTCCTTCATAGTTCTGCCCTGATTGAGCACCAGAAAATCCACTTTGGGGATGACAAAGATAATGA GCGTGAACATGAACGTGAACGTGAACGTGAGCGCGGGGAAACCTTTAGGCCCAGCCCAGCCCTTAATGAG TTTCAGAAAATGTATGGTAAAGAGAAAATGTACGAATGTAAGGTGTGTGGGGAGACTTTCCTTCATAGCT CATCCCTGAAAGAACATCAGAAAATCCATACTAGAGGGAACCCATTTGAAAACAAGGGTAAAGTGTGTGA GGAAACCTTTATTCCTGGTCAGTCCCTTAAAAGGCGTCAGAAAACTTACAATAAGGAGAAGCTCTGTGAC TTTACAGATGGCCGGGATGCCTTCATGCAAAGCTCAGAGCTCAGTGAGCATCAGAAAATTCATTCTCGAA AGAACCTCTTTGAAGGCAGAGGGTATGAGAAATCTGTCATTCATAGTGGGCCATTCACTGAATCTCAGAA GAGTCATACTATAACAAGACCTCTTGAAAGTGATGAGGACGAAAAGGCGTTCACCATTAGCTCTAACCCC TATGAAAACCAGAAGATTCCCACTAAGGAAAATGTCTATGAGGCAAAATCATATGAGAGGTCTGTTATTC ATAGCTTAGCCTCTGTGGAAGCTCAGAAAAGTCACAGTGTAGCAGGGCCCAGTAAACCAAAAGTAATGGC AGAGTCTACCATTCAGAGCTTCGATGCTATCAACCATCAGAGAGTTCGTGCTGGAGGGAACACCTCTGAA GGAAGGGAATACAGTAGGTCTGTTATCCATAGCTTAGTGGCTTCCAAACCTCCAAGAAGTCACAATGGAA ATGAATTGGTGGAATCTAATGAGAAGGGAGAATCCTCCATTTATATCTCAGACCTTAATGATAAGCGACA GAAGATTCCTGCCAGAGAGAACCCTTGTGAAGGGGGCAGTAAGAATCGCAACTATGAAGACTCTGTCATA CAGAGTGTATTCCGTGCCAAACCTCAGAAAAGTGTTCCTGGAGAGGGATCTGGTGAGTTTAAGAAGGATG GCGAATTCTCTGTTCCCAGCTCAAATGTCCGTGAATACCAGAAGGCTCGTGCTAAAAAGAAATACATTGA GCATAGGAGCAATGAGACCTCTGTAATTCACTCTCTGCCTTTTGGTGAACAAACATTTCGCCCTCGAGGG ATGCTCTATGAATGTCAGGAGTGTGGGGAGTGCTTTGCTCATAGCTCTGACCTCACTGAGCACCAGAAGA TTCATGATAGGGAGAAGCCCTCTGGAAGCAGAAACTATGAATGGTCTGTCATTCGCAGCTTGGCCCCTAC TGACCCTCAAACAAGTTACGCCCAAGAGCAGTATGCTAAAGAGCAAGCGCGGAACAAATGTAAGGACTTC AGACAATTTTTTGCTACCAGCGAAGACCTCAACACAAACCAGAAAATCTATGACCAAGAGAAGTCTCATG GCGAGGAGTCTCAAGGCGAGAATACTGATGGGGAGGAGACCCACAGCGAGGAGACCCATGGTCAGGAGAC AATTGAAGACCCTGTCATTCAAGGCTCAGACATGGAAGACCCTCAGAAGGATGACCCTGATGACAAAATC TATGAATGTGAGGACTGTGGCCTGGGCTTTGTGGATCTCACAGACCTCACAGACCATCAGAAAGTCCACA GCAGGAAGTGCCTGGTTGACAGTCGGGAGTACACACATTCTGTAATTCACACCCATTCCATCAGCGAGTA TCAGAGAGATTACACTGGAGAGCAGCTGTATGAATGTCCAAAGTGTGGGGAATCTTTTATTCATAGCTCA TTCCTTTTCGAGCATCAGAGAATCCATGAACAAGACCAGTTGTATTCCATGAAGGGGTGTGATGATGGTT TTATTGCCCTCTTGCCCATGAAGCCACGGAGGAATCGTGCTGCAGAGAGGAATCCTGCTCTTGCTGGGTC GGCCATTCGATGCCTTTTGTGTGGACAAGGCTTCATTCATAGCTCTGCCCTTAATGAGCATATGAGACTT CATAGGGAAGATGATTTACTGGAGCAGAGCCAGATGGCTGAGGAAGCTATCATTCCAGGCTTAGCCCTCA CTGAGTTTCAGAGAAGTCAGACCGAAGAGAGACTCTTTGAATGTGCAGTCTGTGGAGAATCTTTCGTCAA CCCAGCAGAACTTGCAGATCACGTAACTGTTCATAAGAATGAGCCCTATGAGTACGGGTCCTCCTATACT CACACCTCATTTCTTACTGAGCCCCTCAAAGGAGCTATACCATTCTATGAATGCAAGGATTGTGGTAAGT CCTTTATTCATAGCACAGTCCTCACTAAACATAAGGAGCTTCATCTGGAAGAAGAAGAAGAAGATGAAGC AGCAGCAGCTGCAGCAGCAGCAGCCCAGGAAGTTGAAGCCAATGTCCATGTTCCACAAGTAGTTCTGAGG ATTCAGGGCTTAAACGTAGAGGCTGCTGAGCCAGAAGTGGAGGCTGCCGAGCCAGAAGTGGAGGCTGCTG AGCCAGAAGTGGAGGCTGCTGAGCCAAACGGAGAGGCTGAAGGGCCAGATGGAGAGGCTGCAGAGCCCAT TGGAGAGGCTGGACAGCCAAATGGAGAGGCCGAGCAGCCAAATGGGGATGCTGATGAGCCAGATGGTGCA GGTATTGAAGACCCAGAAGAAAGAGCTGAAGAGCCAGAGGGAAAAGCTGAAGAGCCAGAGGGAGATGCCG ACGAGCCTGACGGTGTGGGAATTGAAGACCCAGAAGAAGGTGAAGATCAAGAGATTCAGGTAGAAGAACC ATACTATGACTGCCATGAATGCACAGAAACCTTCACTTCCAGCACAGCATTCAGTGAACACCTGAAAACT CATGCCAGCATGATCATATTTGAGCCTGCAAATGCCTTTGGGGAGTGCTCAGGCTACATCGAACGTGCCA GCACCAGCACAGGTGGTGCCAATCAAGCTGATGAGAAGTACTTCAAATGTGACGTCTGTGGGCAGCTCTT CAATGACCGCCTGTCCCTCGCCAGACACCAGAATACCCACACTGGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC229077 representing NM_001146185 Red=Cloning site Green=Tags(s) MYQPEDDNNSDVTSDDDMTRNRRESSPPHSVHSFSDRDWDRRGRSRDMEPRDRWSHTRNPRSRMPPRDLS LPVVAKTSFEMDREDDRDSRAYESRSQDAESYQNVVDLAEDRKPHNTIQDNMENYRKLLSLVQLAEDDGH SHMTQGHSSRSKRSAYPSTSRGLKTMPEAKKSTHRRGICEDESSHGVIMEKFIKDVSRSSKSGRARESSD RSQRFPRMSDDNWKDISLNKRESVIQQRVYEGNAFRGGFRFNSTLVSRKRVLERKRRYHFDTDGKGSIHD QKGCPRKKPFECGSEMRKAMSVSSLSSLSSPSFTESQPIDFGAMPYVCDECGRSFSVISEFVEHQIMHTR ENLYEYGESFIHSVAVSEVQKSQVGGKRFECKDCGETFNKSAALAEHRKIHARGYLVECKNQECEEAFMP SPTFSELQKIYGKDKFYECRVCKETFLHSSALIEHQKIHFGDDKDNEREHERERERERGETFRPSPALNE FQKMYGKEKMYECKVCGETFLHSSSLKEHQKIHTRGNPFENKGKVCEETFIPGQSLKRRQKTYNKEKLCD FTDGRDAFMQSSELSEHQKIHSRKNLFEGRGYEKSVIHSGPFTESQKSHTITRPLESDEDEKAFTISSNP YENQKIPTKENVYEAKSYERSVIHSLASVEAQKSHSVAGPSKPKVMAESTIQSFDAINHQRVRAGGNTSE GREYSRSVIHSLVASKPPRSHNGNELVESNEKGESSIYISDLNDKRQKIPARENPCEGGSKNRNYEDSVI QSVFRAKPQKSVPGEGSGEFKKDGEFSVPSSNVREYQKARAKKKYIEHRSNETSVIHSLPFGEQTFRPRG MLYECQECGECFAHSSDLTEHQKIHDREKPSGSRNYEWSVIRSLAPTDPQTSYAQEQYAKEQARNKCKDF RQFFATSEDLNTNQKIYDQEKSHGEESQGENTDGEETHSEETHGQETIEDPVIQGSDMEDPQKDDPDDKI YECEDCGLGFVDLTDLTDHQKVHSRKCLVDSREYTHSVIHTHSISEYQRDYTGEQLYECPKCGESFIHSS FLFEHQRIHEQDQLYSMKGCDDGFIALLPMKPRRNRAAERNPALAGSAIRCLLCGQGFIHSSALNEHMRL HREDDLLEQSQMAEEAIIPGLALTEFQRSQTEERLFECAVCGESFVNPAELADHVTVHKNEPYEYGSSYT HTSFLTEPLKGAIPFYECKDCGKSFIHSTVLTKHKELHLEEEEEDEAAAAAAAAAQEVEANVHVPQVVLR IQGLNVEAAEPEVEAAEPEVEAAEPEVEAAEPNGEAEGPDGEAAEPIGEAGQPNGEAEQPNGDADEPDGA GIEDPEERAEEPEGKAEEPEGDADEPDGVGIEDPEEGEDQEIQVEEPYYDCHECTETFTSSTAFSEHLKT HASMIIFEPANAFGECSGYIERASTSTGGANQADEKYFKCDVCGQLFNDRLSLARHQNTHTG TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001146185
ORF Size	4386 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001146185.2
RefSeq Size	8375 bp
RefSeq ORF	4389 bp
Locus ID	5178
UniProt ID	Q9GZU2
Protein Families	Transcription Factors
MW	166 kDa
Gene Summary	In human, ZIM2 and PEG3 are treated as two distinct genes though they share multiple 5' exons and a common promoter and both genes are paternally expressed (PMID:15203203). Alternative splicing events connect their shared 5' exons either with the remaining 4 exons unique to ZIM2, or with the remaining 2 exons unique to PEG3. In contrast, in other mammals ZIM2 does not undergo imprinting and, in mouse, cow, and likely other mammals as well, the ZIM2 and PEG3 genes do not share exons. Human PEG3 protein belongs to the Kruppel C2H2-type zinc finger protein family. PEG3 may play a role in cell proliferation and p53-mediated apoptosis. PEG3 has also shown tumor suppressor activity and tumorigenesis in glioma and ovarian cells. Alternative splicing of this PEG3 gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Sep 2009]

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