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DLC1 (NM_001164271) Human Tagged ORF Clone
CAT＃: RC229057
- TrueORF®
DLC1 (Myc-DDK-tagged)-Human deleted in liver cancer 1 (DLC1), transcript variant 4
ORF Plasmid: tGFP
"NM_001164271" in other vectors (4)
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CNY 10,170.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ARHGAP7; HP; p122-RhoGAP; STARD12 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC229057 representing NM_001164271
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGCTAGAAATTAGTCCTCATCGGAAACGAAGTGACGATTCAGACGAGGATGAGCCTTGTGCCATCA GTGGCAAATGGACTTTCCAAAGGGACAGCAAGAGGTGGTCCCGGCTTGAAGAGTTTGATGTCTTTTCTCC AAAACAAGACCTGGTCCCTGGGTCCCCAGACGACTCCCACCCGAAGGACGGCCCCAGCCCCGGAGGCACG CTGATGGACCTCAGCGAGCGCCAGGAGGTGTCTTCCGTCCGCAGCCTCAGCAGCACTGGCAGCCTCCCCA GCCACGCGCCCCCCAGCGAGGATGCTGCCACCCCCCGGACTAACTCCGTCATCAGCGTTTGCTCCTCCAG CAACTTGGCAGGCAATGACGACTCTTTCGGCAGCCTGCCCTCTCCCAAGGAACTGTCCAGCTTCAGCTTC AGCATGAAAGGCCACGAAAAAACTGCCAAGTCCAAGACGCGCAGTCTGCTGAAACGGATGGAGAGCCTGA AGCTCAAGAGCTCCCATCACAGCAAGCACAAAGCGCCCTCAAAGCTGGGGTTGATCATCAGCGGGCCCAT CTTGCAAGAGGGGATGGATGAGGAGAAGCTGAAGCAGCTCAACTGCGTGGAGATCTCCGCCCTCAATGGC AACCGCATCAACGTCCCCATGGTACGAAAGAGGAGCGTTTCCAACTCCACGCAGACCAGCAGCAGCAGCA GCCAGTCGGAGACCAGCAGCGCGGTCAGCACGCCCAGCCCTGTTACGAGGACCCGGAGCCTCAGTGCGTG CAACAAGCGGGTGGGCATGTACTTAGAGGGCTTCGATCCTTTCAATCAGTCAACATTTAACAACGTGGTG GAGCAGAACTTTAAGAACCGCGAGAGCTACCCAGAGGACACGGTGTTCTACATCCCTGAAGATCACAAGC CTGGCACTTTCCCCAAAGCTCTCACCAATGGCAGTTTCTCCCCCTCGGGGAATAACGGCTCTGTGAACTG GAGGACGGGAAGCTTCCACGGCCCTGGCCACATCAGCCTCAGGAGGGAAAACAGTAGCGACAGCCCCAAG GAACTGAAGAGACGCAATTCTTCCAGCTCCATGAGCAGCCGCCTGAGCATCTACGACAACGTGCCGGGCT CCATCCTCTACTCCAGTTCAGGGGACCTGGCGGATCTGGAGAACGAGGACATCTTCCCCGAGCTGGACGA CATCCTCTACCACGTGAAGGGGATGCAGCGGATAGTCAATCAGTGGTCGGAGAAGTTTTCTGATGAGGGA GATTCGGACTCAGCCCTGGACTCGGTCTCTCCCTGCCCGTCCTCTCCAAAACAGATACACCTGGATGTGG ACAACGACCGAACCACACCCAGCGACCTGGACAGCACAGGCAACTCCCTGAATGAACCGGAAGAGCCCTC CGAGATCCCGGAAAGAAGGGATTCTGGGGTTGGGGCTTCCCTAACCAGGTCCAACAGGCACCGACTGAGA TGGCACAGTTTCCAGAGCTCACATCGGCCAAGCCTCAACTCTGTATCACTACAGATTAACTGCCAGTCTG TGGCCCAGATGAACCTGCTGCAGAAATACTCACTCCTAAAGCTAACGGCCCTGCTGGAGAAATACACACC TTCTAACAAGCATGGTTTTAGCTGGGCCGTGCCCAAGTTCATGAAGAGGATCAAGGTTCCAGACTACAAG GACCGGAGTGTGTTTGGGGTCCCACTGACGGTCAACGTGCAGCGCACAGGACAACCGTTGCCTCAGAGCA TCCAGCAGGCCATGCGATACCTCCGGAACCATTGTTTGGATCAGGTTGGGCTCTTCAGAAAATCGGGGGT CAAGTCCCGGATTCAGGCTCTGCGCCAGATGAATGAAGGTGCCATAGACTGTGTCAACTACGAAGGACAG TCTGCTTATGACGTGGCAGACATGCTGAAGCAGTATTTTCGAGATCTTCCTGAGCCACTAATGACGAACA AACTCTCGGAAACCTTTCTACAGATCTACCAATATGTGCCCAAGGACCAGCGCCTGCAGGCCATCAAGGC TGCCATCATGCTGCTGCCTGACGAGAACCGGGAGGTTCTGCAGACCCTGCTTTATTTCCTGAGCGATGTC ACAGCAGCCGTAAAAGAAAACCAGATGACCCCAACCAACCTGGCCGTGTGCTTAGCGCCTTCCCTCTTCC ATCTCAACACCCTGAAGAGAGAGAATTCCTCTCCCAGGGTAATGCAAAGAAAACAAAGTTTGGGCAAACC AGATCAGAAAGATTTGAATGAAAACCTAGCTGCCACTCAAGGGCTGGCCCATATGATCGCCGAGTGCAAG AAGCTTTTCCAGGTTCCCGAGGAAATGAGCCGATGTCGTAATTCCTATACCGAACAAGAGCTGAAGCCCC TCACTCTGGAAGCACTCGGGCACCTGGGTAATGATGACTCAGCTGACTACCAACACTTCCTCCAGGACTG TGTGGATGGCCTGTTTAAAGAAGTCAAAGAGAAGTTTAAAGGCTGGGTCAGCTACTCCACTTCGGAGCAG GCTGAGCTGTCCTATAAGAAGGTGAGCGAAGGACCCCCTCTGAGGCTTTGGAGGTCAGTCATTGAAGTCC CTGCTGTGCCAGAGGAAATCTTAAAGCGCCTACTTAAAGAACAGCACCTCTGGGATGTAGACCTGTTGGA TTCAAAAGTGATCGAAATTCTGGACAGCCAAACTGAAATTTACCAGTATGTCCAAAACAGTATGGCACCT CATCCTGCTCGAGACTACGTTGTTTTAAGAACCTGGAGGACTAATTTACCCAAAGGAGCCTGTGCCCTTT TACTAACCTCTGTGGATCACGATCGCGCACCTGTGGTGGGTGTGAGGGTTAATGTGCTCTTGTCCAGGTA TTTGATTGAACCCTGTGGGCCAGGAAAATCCAAACTCACCTACATGTGCAGAGTTGACTTAAGGGGCCAC ATGCCAGAATGGTACACAAAATCTTTTGGACATTTGTGTGCAGCTGAAGTTGTAAAGATCCGGGATTCCT TCAGTAACCAGAACACTGAAACCAAAGACACCAAATCTAGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC229057 representing NM_001164271
Red=Cloning site Green=Tags(s) MKLEISPHRKRSDDSDEDEPCAISGKWTFQRDSKRWSRLEEFDVFSPKQDLVPGSPDDSHPKDGPSPGGT LMDLSERQEVSSVRSLSSTGSLPSHAPPSEDAATPRTNSVISVCSSSNLAGNDDSFGSLPSPKELSSFSF SMKGHEKTAKSKTRSLLKRMESLKLKSSHHSKHKAPSKLGLIISGPILQEGMDEEKLKQLNCVEISALNG NRINVPMVRKRSVSNSTQTSSSSSQSETSSAVSTPSPVTRTRSLSACNKRVGMYLEGFDPFNQSTFNNVV EQNFKNRESYPEDTVFYIPEDHKPGTFPKALTNGSFSPSGNNGSVNWRTGSFHGPGHISLRRENSSDSPK ELKRRNSSSSMSSRLSIYDNVPGSILYSSSGDLADLENEDIFPELDDILYHVKGMQRIVNQWSEKFSDEG DSDSALDSVSPCPSSPKQIHLDVDNDRTTPSDLDSTGNSLNEPEEPSEIPERRDSGVGASLTRSNRHRLR WHSFQSSHRPSLNSVSLQINCQSVAQMNLLQKYSLLKLTALLEKYTPSNKHGFSWAVPKFMKRIKVPDYK DRSVFGVPLTVNVQRTGQPLPQSIQQAMRYLRNHCLDQVGLFRKSGVKSRIQALRQMNEGAIDCVNYEGQ SAYDVADMLKQYFRDLPEPLMTNKLSETFLQIYQYVPKDQRLQAIKAAIMLLPDENREVLQTLLYFLSDV TAAVKENQMTPTNLAVCLAPSLFHLNTLKRENSSPRVMQRKQSLGKPDQKDLNENLAATQGLAHMIAECK KLFQVPEEMSRCRNSYTEQELKPLTLEALGHLGNDDSADYQHFLQDCVDGLFKEVKEKFKGWVSYSTSEQ AELSYKKVSEGPPLRLWRSVIEVPAVPEEILKRLLKEQHLWDVDLLDSKVIEILDSQTEIYQYVQNSMAP HPARDYVVLRTWRTNLPKGACALLLTSVDHDRAPVVGVRVNVLLSRYLIEPCGPGKSKLTYMCRVDLRGH MPEWYTKSFGHLCAAEVVKIRDSFSNQNTETKDTKSR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001164271 |
| ORF Size | 3051 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001164271.1, NP_001157743.1 |
| RefSeq Size | 5786 bp |
| RefSeq ORF | 3054 bp |
| Locus ID | 10395 |
| UniProt ID | Q96QB1 |
| MW | 114.2 kDa |
| Gene Summary | This gene encodes a GTPase-activating protein (GAP) that is a member of the rhoGAP family of proteins which play a role in the regulation of small GTP-binding proteins. GAP family proteins participate in signaling pathways that regulate cell processes involved in cytoskeletal changes. This gene functions as a tumor suppressor gene in a number of common cancers, including prostate, lung, colorectal, and breast cancers. Multiple transcript variants due to alternative promoters and alternative splicing have been found for this gene.[provided by RefSeq, Apr 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC229057L3 | Lenti-ORF clone of DLC1 (Myc-DDK-tagged)-Human deleted in liver cancer 1 (DLC1), transcript variant 4 |
CNY 12,070.00 |
|
| RC229057L4 | Lenti-ORF clone of DLC1 (mGFP-tagged)-Human deleted in liver cancer 1 (DLC1), transcript variant 4 |
CNY 12,070.00 |
|
| RG229057 | DLC1 (tGFP-tagged) - Human deleted in liver cancer 1 (DLC1), transcript variant 4 |
CNY 11,210.00 |
|
| SC327692 | DLC1 (untagged)-Human deleted in liver cancer 1 (DLC1) transcript variant 4 |
CNY 10,450.00 |
