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KRT72 (NM_001146225) Human Tagged ORF Clone
CAT＃: RC228937
- TrueORF®
KRT72 (Myc-DDK-tagged)-Human keratin 72 (KRT72), transcript variant 2
ORF Plasmid: tGFP
"NM_001146225" in other vectors (4)
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CNY 3,808.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CK-72; K6irs; K6IRS2; K72; KRT6; KRT6IRS2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC228937 representing NM_001146225
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCCGCCAACTGACCCATTTCCCCCGCGGGGAGCGCCTGGGCTTCAGCGGTTGCTCCGCGGTCCTCT CTGGCGGGATCGGCAGCAGCTCCGCCTCATTCCGGGCCCGGGTCAAGGGCTCGGCCTCCTTTGGCAGCAA GAGCCTCTCCTGCCTTGGGGGCAGCCGAAGCCTGGCGCTCAGCGCTGCTGCACGGCGGGGCGGCGGCCGC CTGGGCGGCTTCGTGGGCACCGCCTTCGGCAGCGCCGGGCTGGGGCCCAAGTGTCCCTCCGTGTGCCCAC CCGGGGGCATCCCTCAGGTCACCGTCAACAAGAGCCTCCTGGCCCCGCTCAACGTGGAGATGGACCCCGA GATCCAGAGGGTGCGCGCCCAGGAGCGGGAGCAGATCAAGGCGCTAAACAACAAGTTCGCCTCCTTCATC GACAAGGTGCGGTTCCTGGAGCAGCAGAATCAGGTGCTAGAGACCAAGTGGAACCTCCTACAGCAGCTGG ACTTGAACAACTGCAGGAAGAACCTGGAGCCCATTTATGAGGGCTACATCAGCAACCTGCAGAAGCAGCT GGAGATGCTGTCTGGGGACGGGGTGAGGCTGGATTCGGAGCTGAGGAACATGCAGGATTTGGTGGAGGAC TACAAGAAGAGGTATGAGGTGGAGATTAACAGACGCACAGCTGCTGAGAATGAGTTTGTGGTGCTCAAGA AGGACGTGGATGCTGCTTACATGAATAAGGTTGAGCTCCAGGCCAAGGTGGACTCCTTGACAGATGAGAT TAAATTCTTCAAGTGCCTTTATGAAGGGGAGATCACTCAGATCCAGTCCCACATCAGCGACACGTCCATC GTCCTGTCAATGGACAACAACCGGGATCTGGACCTGGACAGCATCATTGCCGAGGTCCGTGCCCAGTACG AGGAGATTGCCCTAAAGAGCAAGGCCGAGGCTGAGACCCTGTACCAGACCAAGATCCAGGAGCTGCAGGT CACAGCAGGCCAGCATGGGGATGACCTCAAGCTCACCAAGGCTGAAATCTCTGAGCTCAACCGCCTGATC CAGAGGATCCGCTCAGAGATAGGGAATGTGAAGAAGCAGTGTGCCGATCTGGAGACGGCCATCGCCGACG CTGAACAGCGGGGGGACTGCGCCCTGAAAGATGCCCGGGCCAAGCTGGATGAGCTGGAGGGCGCCCTGCA CCAGGCCAAGGAGGAGCTGGCACGGATGCTGCGTGAGTACCAGGAGCTCGTGAGCCTGAAGCTGGCCCTG GATATGGAGATCGCCACCTACCGCAAGCTGCTGGAGAGCGAGGAGTGCAGGATGTCTGGCGAATATCCAA ATTCTGTGAGCATCTCCGTCATCAGCAGCACCAATGCTGGGGCAGGAGGGGCTGGCTTCAGCATGGGCTT TGGCGCCTCAAGCAGTTATAGCTACAAAACTGCAGCTGCAGACGTCAAGACCAAAGGCAGCTGTGGCAGT GAGCTCAAGGATCCCCTTGCCAAAACCTCGGGGAGCAGCTGTGCCACCAAAAAGGCCTCCAGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC228937 representing NM_001146225
Red=Cloning site Green=Tags(s) MSRQLTHFPRGERLGFSGCSAVLSGGIGSSSASFRARVKGSASFGSKSLSCLGGSRSLALSAAARRGGGR LGGFVGTAFGSAGLGPKCPSVCPPGGIPQVTVNKSLLAPLNVEMDPEIQRVRAQEREQIKALNNKFASFI DKVRFLEQQNQVLETKWNLLQQLDLNNCRKNLEPIYEGYISNLQKQLEMLSGDGVRLDSELRNMQDLVED YKKRYEVEINRRTAAENEFVVLKKDVDAAYMNKVELQAKVDSLTDEIKFFKCLYEGEITQIQSHISDTSI VLSMDNNRDLDLDSIIAEVRAQYEEIALKSKAEAETLYQTKIQELQVTAGQHGDDLKLTKAEISELNRLI QRIRSEIGNVKKQCADLETAIADAEQRGDCALKDARAKLDELEGALHQAKEELARMLREYQELVSLKLAL DMEIATYRKLLESEECRMSGEYPNSVSISVISSTNAGAGGAGFSMGFGASSSYSYKTAAADVKTKGSCGS ELKDPLAKTSGSSCATKKASR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001146225 |
| ORF Size | 1533 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001146225.2 |
| RefSeq Size | 1903 bp |
| RefSeq ORF | 1536 bp |
| Locus ID | 140807 |
| UniProt ID | Q14CN4 |
| MW | 55.9 kDa |
| Gene Summary | Keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells. The type II keratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This gene encodes a type II keratin that is specifically expressed in the inner root sheath of hair follicles. The type II keratins are clustered in a region of chromosome 12q12-q13. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jun 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC228937L3 | Lenti-ORF clone of KRT72 (Myc-DDK-tagged)-Human keratin 72 (KRT72), transcript variant 2 |
CNY 5,890.00 |
|
| RC228937L4 | Lenti-ORF clone of KRT72 (mGFP-tagged)-Human keratin 72 (KRT72), transcript variant 2 |
CNY 5,890.00 |
|
| RG228937 | KRT72 (tGFP-tagged) - Human keratin 72 (KRT72), transcript variant 2 |
CNY 4,370.00 |
|
| SC327572 | KRT72 (untagged)-Human keratin 72 (KRT72) transcript variant 2 |
CNY 7,220.00 |
