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AFAF (EQTN) (NM_001161585) Human Tagged ORF Clone
CAT＃: RC228789
EQTN (Myc-DDK-tagged)-Human chromosome 9 open reading frame 11 (C9orf11), transcript variant 2
ORF Plasmid: tGFP
"NM_001161585" in other vectors (4)
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CNY 2,400.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AFAF; C9orf11; SPACA8 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC228789 representing NM_001161585
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATTTTATATTGTTTATTTTTATACCTGGAGTTTTTTCCTTAAAAAGTAGCACTTTGAAGCCTACTA TTGAAGCATTGCCTAATGTGCTACCTTTAAATGAAGATGTTAATAAGCAGGAAGAAAAGAATGAAGATCA TACTCCCAATTATGCTCCTGCTAATGAGAAAAATGGCAATTATTATAAAGATATAAAACAATATGTGTTC ACAACACAAAATCCAAATGGCACTGAGTCTGAAATATCTGTGAGAGCCACAACTGACCTGAATTTTGCTC TAAAAAACGGATCAACCCCAAACGTGCCTGCATTTTGGACAATGTTAGCTAAAGCTATAAATGGAACAGC AGTGGTCATGGATGATAAAGATCAATTATTTCACCCAATTCCAGAGTCTGATGTGAATGCTACACAGGGA GAAAATCAGCCAGATCTAGAGGATCTGAAGATCAAAATAATGCTGGGAATCTCGTTGATGACCCTCCTCC TCTTTGTGGTCCTCTTGGCATTCTGTAGTGCTACACTGTACAAACTGAGGCATCTGAGTTATAAAAGTTG TGAGAGTCAGTACTCTGTCAACCCAGAGCTGGCCACGATGTCTTACTTTCATCCATCAGAAGGTGTTTCA GATACATCCTTTTCCAAGAGTGCAGAGAGCAGCACATTTTTGGGTACCACTTCTTCAGATATGAGAAGAT CAGGCACAAGAACATCAGAATCTAAGATAATGACGGATATCATTTCCATAGGCTCAGATAATGAGATGCA TGAAAACGATGAGTCGGTTACCCGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC228789 representing NM_001161585
Red=Cloning site Green=Tags(s) MNFILFIFIPGVFSLKSSTLKPTIEALPNVLPLNEDVNKQEEKNEDHTPNYAPANEKNGNYYKDIKQYVF TTQNPNGTESEISVRATTDLNFALKNGSTPNVPAFWTMLAKAINGTAVVMDDKDQLFHPIPESDVNATQG ENQPDLEDLKIKIMLGISLMTLLLFVVLLAFCSATLYKLRHLSYKSCESQYSVNPELATMSYFHPSEGVS DTSFSKSAESSTFLGTTSSDMRRSGTRTSESKIMTDIISIGSDNEMHENDESVTR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001161585 |
| ORF Size | 795 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001161585.1, NP_001155057.1 |
| RefSeq ORF | 798 bp |
| Locus ID | 54586 |
| UniProt ID | Q9NQ60 |
| Protein Families | Transmembrane |
| MW | 29.3 kDa |
| Gene Summary | Acrosomal membrane-anchored protein involved in the process of fertilization and in acrosome biogenesis.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC228789L3 | Lenti ORF clone of Human chromosome 9 open reading frame 11 (C9orf11), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC228789L4 | Lenti ORF clone of Human chromosome 9 open reading frame 11 (C9orf11), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG228789 | EQTN (tGFP-tagged) - Human chromosome 9 open reading frame 11 (C9orf11), transcript variant 2 |
CNY 4,370.00 |
|
| SC327424 | EQTN (untagged)-Human chromosome 9 open reading frame 11 (C9orf11) transcript variant 2 |
CNY 3,990.00 |
