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EVC2 (NM_001166136) Human Tagged ORF Clone
CAT＃: RC228654
- TrueORF®
EVC2 (Myc-DDK-tagged)-Human Ellis van Creveld syndrome 2 (EVC2), transcript variant 2
ORF Plasmid: tGFP
"NM_001166136" in other vectors (4)
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CNY 8,552.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | LBN; WAD |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC228654 representing NM_001166136
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATTTGGCCCAAAGTGGAATGCTGTCACTTTAAGACTGCAGTGGAAGCACCACTTGGAATGAAATTGG ACAAGAAAATGGAAGTCTTCATCCCACTCTCAACTTCTGCAGCCTCTAGTGGGCCATGGGCTCATTCCTT ATTTGCTTTTATACCCTCCTGGCCTAAGAAGAACTTATTTAAAAGAGAGTCTCCTATAACACACCGCCTG TATGGGGACATTTCAAGAGAAGTTCAAGGGACTTCTGAAAATGGAGTAATTTTTCAGAAATGTGCACTGG TGTCTGGGTCGAGTGAAGCACAGACAGCCCGCATATGGCTGCTTGTTAACAACACCAAGACAACCTCGTC AGCCAACCTCTCGGAGCTGCTCTTGCTGGACAGCATTGCTGGTCTCACCATTTGGGACTCTGTGGGAAAC AGGACCTCGGAAGGATTCCAGGCTTTTAGCAAGAAGTTTCTGCAAGTGGGAGATGCCTTTGCTGTCAGCT ACGCAGCCACGCTCCAGGCTGGAGACCTCGGGAACGGGGAGAGCCTCAAGCTTCCTGCCCAACTCACCTT TCAGAGCTCGTCACGGAACAGAACACAGCTGAAAGTGCTTTTTTCCATAACAGCAGAAGAAAACGTAACG GTTCTGCCGCACCACGGCCTCCACGCAGCAGGGTTCTTCATTGCCTTCCTCCTCTCCCTTGTGCTGACCT GGGCTGCCCTCTTCCTCATGGTTCGCTATCAGTGTCTGAAGGGAAACATGCTCACCAGACATCGGGTTTG GCAGTATGAGAGCAAGCTGGAACCCTTGCCGTTCACCTCAGCTGATGGCGTGAATGAGGACCTTTCCCTT AACGACCAAATGATAGACATTCTGTCTTCCGAGGACCCTGGGAGCATGCTTCAAGCCTTAGAAGAGTTGG AGATTGCAACCCTGAATCGGGCAGATGCAGATCTGGAGGCTTGTCGAACACAAATCAGCAAGGATATCAT TGCCCTTCTGCTGAAAAATCTCACCAGCAGTGGCCACCTCTCACCCCAAGTAGAGAGAAAAATGAGTGCT GTTTTCAAAAAGCAGTTTCTATTGCTGGAAAATGAAATACAAGAGGAGTACGATCGGAAGATGGTGGCAT TGACAGCTGAATGTGACCTGGAAACAAGAAAGAAGATGGAAAACCAGTACCAGAGAGAGATGATGGCAAT GGAGGAAGCAGAAGAGTTGCTGAAACGTGCTGGTGAGAGGTCTGCTGTAGAGTGCAGCAACCTTCTGCGG ACCCTCCATGGCCTGGAACAGGAGCACTTGAGGAAGTCTCTCGCTTTGCAACAAGAAGAAGACTTTGCCA AAGCTCACAGACAGCTGGCTGTTTTCCAGAGAAATGAACTGCACAGTATCTTTTTTACCCAGATAAAAAG TGCTATTTTCAAAGGGGAATTGAAACCAGAGGCAGCTAAAATGCTGCTGCAAAATTATTCTAAAATACAG GAGAATGTAGAAGAGTTAATGGACTTTTTCCAGGCTAGTAAGAGGTATCATCTAAGTAAAAGATTTGGCC ACAGGGAATATCTGGTCCAGAACCTCCAGTCATCAGAGACCCGTGTGCAGGGCCTTCTGAGCACCGCTGC AGCCCAGCTGACTCACCTCATTCAGAAGCACGAGAGAGCAGGGTACCTGGATGAAGACCAAATGGAAATG CTATTGGAGCGGGCTCAGACAGAAGTCTTTTCAATCAAGCAGAAGTTGGACAATGACTTAAAGCAGGAAA AGAAAAAGCTCCACCAAAAATTAATAACTAAGAGAAGACGAGAGTTGCTACAAAAGCACAGGGAGCAGCG TAGGGAGCAGGCGTCCGTCGGCGAGGCCTTCCGAACGGTTGAGGATGCCGGCCAGTACCTGCACCAGAAG AGGAGCCTGATGGAGGAGCACGGTGCCACCCTGGAGGAGCTGCAGGAGCGTCTGGACCAGGCCGCCCTGG ACGATCTCAGGACCCTGACCCTTTCGCTGTTTGAAAAGGCCACCGACGAGCTGCGGCGCCTGCAGAACTC AGCCATGACCCAGGAGCTGCTCAAGCGTGGGGTGCCCTGGCTCTTCCTGCAGCAGATCCTGGAGGAGCAC GGCAAGGAGATGGCTGCACGGGCCGAGCAGCTGGAGGGGGAGGAGAGGGACAGGGACCAGGAGGGTGTCC AGAGCGTGAGGCAGAGACTGAAGGATGACGCTCCTGAGGCCGTGACAGAGGAGCAGGCAGAGCTGCGACG CTGGGAGCACCTGATCTTCATGAAGCTCTGCTCCTCAGTCTTCTCCCTGTCTGAAGAGGAGCTGCTCAGG ATGAGGCAGGAGGTCCATGGCTGCTTTGCTCAGATGGACAGGAGCTTGGCCCTCCCCAAGATCCGGGCCC GAGTTCTGCTGCAGCAATTTCAGACTGCGTGGCGAGAAGCAGAGTTCGTGAAGCTGGACCAGGCCGTGGC TGCCCCTGAGCTGCAGCAACAGTCCAAGGTGAGAAAGTCACGGTCCAAGAGTAAAAGCAAGGGAGAGCTT CTGAAGAAGTGCATCGAAGACAAAATTCACCTCTGTGAGGAACAGGCCTCTGAAGACCTGGTGGAAAAGG TTCGAGGTGAATTGCTGCGGGAGAGAGTGCAGCGGATGGAGGCACAGGAGGGAGGCTTTGCACAGTCGCT TGTTGCTCTGCAGTTCCAGAAGGCGTCCCGGGTGACCGAGACTCTGTCGGCCTACACCGCCCTCCTCAGC ATCCAGGACTTGCTCCTGGAAGAGCTGAGTGCATCTGAGATGCTGACCAAGTCGGCCTGCACACAGATCC TGGAGTCGCACAGCCGGGAGCTCCAGGAGTTGGAGAGGAAGCTGGAGGACCAGCTGGTGCAGCAGGAGGC AGCCCAGCAGCAGCAGGCCCTGGCGAGCTGGCAGCAGTGGGTGGCCGATGGGCCCGGGATTCTGAACGAA CCTGGGGAGGTGGATTCTGAAAGGCAGGTCTCTACTGTCCTGCACCAAGCCCTGAGCAAGAGCCAGACAT TACTGGAGCAACATCAGCAGTGTTTGAGAGAGGAACAACAGAACAGTGTCGTGCTAGAAGACTTGTTGGA AAACATGGAGGCAGACACCTTTGCAACCCTGTGCAGCCAGGAGCTGAGACTGGCATCGTACCTGGCGAGG ATGGCCATGGTGCCCGGGGCCACGCTTCGCCGGCTCCTGAGTGTGGTACTGCCCACAGCCTCACAGCCTC AGCTGCTGGCCCTGCTGGATTCGGCCACCGAGAGACATGTGGACCACGCAGCTGAGAGCGATGGCGGAGC GGAGCAGGCCGACGTGGGCAGGCGGAGGAAACACCAGAGCTGGTGGCAAGCCTTAGATGGCAAACTGCGA GGAGATCTGATAAGCAGAGGATTAGAAAAGATGCTGTGGGCCCGCAAGAGAAAGCAGAGCATATTAAAGA AGACATGTCTCCCTCTCAGAGAGAGGATGATATTCTCTGGAAAAGGAAGTTGGCCACACCTGTCACTGGA GCCCATTGGCGAACTGGCCCCTGTACCCATTGTAGGGGCAGAAACCATTGATCTATTAAACACAGGAGAG AAGCTCTTTATATTCAGAAATCCAAAGGAGCCAGAGATCTCACTGCACGTTCCTCCCAGGAAAAAGAAGA ACTTTTTGAATGCCAAAAAGGCCATGAGGGCCTTGGGCATGGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC228654 representing NM_001166136
Red=Cloning site Green=Tags(s) MIWPKVECCHFKTAVEAPLGMKLDKKMEVFIPLSTSAASSGPWAHSLFAFIPSWPKKNLFKRESPITHRL YGDISREVQGTSENGVIFQKCALVSGSSEAQTARIWLLVNNTKTTSSANLSELLLLDSIAGLTIWDSVGN RTSEGFQAFSKKFLQVGDAFAVSYAATLQAGDLGNGESLKLPAQLTFQSSSRNRTQLKVLFSITAEENVT VLPHHGLHAAGFFIAFLLSLVLTWAALFLMVRYQCLKGNMLTRHRVWQYESKLEPLPFTSADGVNEDLSL NDQMIDILSSEDPGSMLQALEELEIATLNRADADLEACRTQISKDIIALLLKNLTSSGHLSPQVERKMSA VFKKQFLLLENEIQEEYDRKMVALTAECDLETRKKMENQYQREMMAMEEAEELLKRAGERSAVECSNLLR TLHGLEQEHLRKSLALQQEEDFAKAHRQLAVFQRNELHSIFFTQIKSAIFKGELKPEAAKMLLQNYSKIQ ENVEELMDFFQASKRYHLSKRFGHREYLVQNLQSSETRVQGLLSTAAAQLTHLIQKHERAGYLDEDQMEM LLERAQTEVFSIKQKLDNDLKQEKKKLHQKLITKRRRELLQKHREQRREQASVGEAFRTVEDAGQYLHQK RSLMEEHGATLEELQERLDQAALDDLRTLTLSLFEKATDELRRLQNSAMTQELLKRGVPWLFLQQILEEH GKEMAARAEQLEGEERDRDQEGVQSVRQRLKDDAPEAVTEEQAELRRWEHLIFMKLCSSVFSLSEEELLR MRQEVHGCFAQMDRSLALPKIRARVLLQQFQTAWREAEFVKLDQAVAAPELQQQSKVRKSRSKSKSKGEL LKKCIEDKIHLCEEQASEDLVEKVRGELLRERVQRMEAQEGGFAQSLVALQFQKASRVTETLSAYTALLS IQDLLLEELSASEMLTKSACTQILESHSRELQELERKLEDQLVQQEAAQQQQALASWQQWVADGPGILNE PGEVDSERQVSTVLHQALSKSQTLLEQHQQCLREEQQNSVVLEDLLENMEADTFATLCSQELRLASYLAR MAMVPGATLRRLLSVVLPTASQPQLLALLDSATERHVDHAAESDGGAEQADVGRRRKHQSWWQALDGKLR GDLISRGLEKMLWARKRKQSILKKTCLPLRERMIFSGKGSWPHLSLEPIGELAPVPIVGAETIDLLNTGE KLFIFRNPKEPEISLHVPPRKKKNFLNAKKAMRALGMD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001166136 |
| ORF Size | 3684 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001166136.1, NP_001159608.1 |
| RefSeq Size | 4864 bp |
| RefSeq ORF | 3687 bp |
| Locus ID | 132884 |
| UniProt ID | Q86UK5 |
| Protein Families | Transmembrane |
| MW | 139.9 kDa |
| Gene Summary | This gene encodes a protein that functions in bone formation and skeletal development. Mutations in this gene, as well as in a neighboring gene that lies in a head-to-head configuration, cause Ellis-van Creveld syndrome, an autosomal recessive skeletal dysplasia that is also known as chondroectodermal dysplasia. Mutations in this gene also cause acrofacial dysostosis Weyers type, also referred to as Curry-Hall syndrome, a disease that combines limb and facial abnormalities. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC228654L3 | Lenti-ORF clone of EVC2 (Myc-DDK-tagged)-Human Ellis van Creveld syndrome 2 (EVC2), transcript variant 2 |
CNY 14,160.00 |
|
| RC228654L4 | Lenti-ORF clone of EVC2 (mGFP-tagged)-Human Ellis van Creveld syndrome 2 (EVC2), transcript variant 2 |
CNY 14,160.00 |
|
| RG228654 | EVC2 (tGFP-tagged) - Human Ellis van Creveld syndrome 2 (EVC2), transcript variant 2 |
CNY 13,490.00 |
|
| SC327289 | EVC2 (untagged)-Human Ellis van Creveld syndrome 2 (EVC2) transcript variant 2 |
CNY 23,660.00 |
