eNOS (NOS3) (NM_001160109) Human Tagged ORF Clone
CAT#: RC228481
- TrueORF®
NOS3 (Myc-DDK-tagged)-Human nitric oxide synthase 3 (endothelial cell) (NOS3), transcript variant 2
ORF Plasmid: tGFP
"NM_001160109" in other vectors (4)
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CNY 4,440.00
CNY 4,560.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | ECNOS; eNOS |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC228481 representing NM_001160109
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCAACTTGAAGAGCGTGGCCCAGGAGCCTGGGCCACCCTGCGGCCTGGGGCTGGGGCTGGGCCTTG GGCTGTGCGGCAAGCAGGGCCCAGCCACCCCGGCCCCTGAGCCCAGCCGGGCCCCAGCATCCCTACTCCC ACCAGCGCCAGAACACAGCCCCCCGAGCTCCCCGCTAACCCAGCCCCCAGAGGGGCCCAAGTTCCCTCGT GTGAAGAACTGGGAGGTGGGGAGCATCACCTATGACACCCTCAGCGCCCAGGCGCAGCAGGATGGGCCCT GCACCCCAAGACGCTGCCTGGGCTCCCTGGTATTTCCACGGAAACTACAGGGCCGGCCCTCCCCCGGCCC CCCGGCCCCTGAGCAGCTGCTGAGTCAGGCCCGGGACTTCATCAACCAGTACTACAGCTCCATTAAGAGG AGCGGCTCCCAGGCCCACGAACAGCGGCTTCAAGAGGTGGAAGCCGAGGTGGCAGCCACAGGCACCTACC AGCTTAGGGAGAGCGAGCTGGTGTTCGGGGCTAAGCAGGCCTGGCGCAACGCTCCCCGCTGCGTGGGCCG GATCCAGTGGGGGAAGCTGCAGGTGTTCGATGCCCGGGACTGCAGGTCTGCACAGGAAATGTTCACCTAC ATCTGCAACCACATCAAGTATGCCACCAACCGGGGCAACCTTCGCTCGGCCATCACAGTGTTCCCGCAGC GCTGCCCTGGCCGAGGAGACTTCCGAATCTGGAACAGCCAGCTGGTGCGCTACGCGGGCTACCGGCAGCA GGATGGCTCTGTGCGGGGGGACCCAGCCAACGTGGAGATCACCGAGCTCTGCATTCAGCACGGCTGGACC CCAGGAAACGGTCGCTTCGACGTGCTGCCCCTGCTGCTGCAGGCCCCAGATGATCCCCCAGAACTCTTCC TTCTGCCCCCCGAGCTGGTCCTTGAGGTGCCCCTGGAGCACCCCACGCTGGAGTGGTTTGCAGCCCTGGG CCTGCGCTGGTACGCCCTCCCGGCAGTGTCCAACATGCTGCTGGAAATTGGGGGCCTGGAGTTCCCCGCA GCCCCCTTCAGTGGCTGGTACATGAGCACTGAGATCGGCACGAGGAACCTGTGTGACCCTCACCGCTACA ACATCCTGGAGGATGTGGCTGTCTGCATGGACCTGGATACCCGGACCACCTCGTCCCTGTGGAAAGACAA GGCAGCAGTGGAAATCAACGTGGCCGTGCTGCACAGTTACCAGCTAGCCAAAGTCACCATCGTGGACCAC CACGCCGCCACGGCCTCTTTCATGAAGCACCTGGAGAATGAGCAGAAGGCCAGGGGGGGCTGCCCTGCAG ACTGGGCCTGGATCGTGCCCCCCATCTCGGGCAGCCTCACTCCTGTTTTCCATCAGGAGATGGTCAACTA TTTCCTGTCCCCGGCCTTCCGCTACCAGCCAGACCCCTGGAAGGGGAGTGCCGCCAAGGGCACCGGCATC ACCAGGAAGAAGACCTTTAAAGAAGTGGCCAACGCCGTGAAGATCTCCGCCTCGCTCATGGGCACGGTGA TGGCGAAGCGAGTGAAGGCGACAATCCTGTATGGCTCCGAGACCGGCCGGGCCCAGAGCTACGCACAGCA GCTGGGGAGACTCTTCCGGAAGGCTTTTGATCCCCGGGTCCTGTGTATGGATGAGTATGACGTGGTGTCC CTCGAACACGAGACGCTGGTGCTGGTGGTAACCAGCACATTTGGGAATGGGGATCCCCCGGAGAATGGAG AGTCTGTCTCCCTGCCAGAAGTGTCTGTCACCACAGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC228481 representing NM_001160109
Red=Cloning site Green=Tags(s) MGNLKSVAQEPGPPCGLGLGLGLGLCGKQGPATPAPEPSRAPASLLPPAPEHSPPSSPLTQPPEGPKFPR VKNWEVGSITYDTLSAQAQQDGPCTPRRCLGSLVFPRKLQGRPSPGPPAPEQLLSQARDFINQYYSSIKR SGSQAHEQRLQEVEAEVAATGTYQLRESELVFGAKQAWRNAPRCVGRIQWGKLQVFDARDCRSAQEMFTY ICNHIKYATNRGNLRSAITVFPQRCPGRGDFRIWNSQLVRYAGYRQQDGSVRGDPANVEITELCIQHGWT PGNGRFDVLPLLLQAPDDPPELFLLPPELVLEVPLEHPTLEWFAALGLRWYALPAVSNMLLEIGGLEFPA APFSGWYMSTEIGTRNLCDPHRYNILEDVAVCMDLDTRTTSSLWKDKAAVEINVAVLHSYQLAKVTIVDH HAATASFMKHLENEQKARGGCPADWAWIVPPISGSLTPVFHQEMVNYFLSPAFRYQPDPWKGSAAKGTGI TRKKTFKEVANAVKISASLMGTVMAKRVKATILYGSETGRAQSYAQQLGRLFRKAFDPRVLCMDEYDVVS LEHETLVLVVTSTFGNGDPPENGESVSLPEVSVTTE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001160109 |
ORF Size | 1788 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001160109.2 |
RefSeq ORF | 1791 bp |
Locus ID | 4846 |
UniProt ID | P29474 |
Protein Families | Druggable Genome |
Protein Pathways | Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Arginine and proline metabolism, Calcium signaling pathway, Long-term depression, Metabolic pathways, Pathways in cancer, Small cell lung cancer, VEGF signaling pathway |
MW | 65.4 kDa |
Gene Summary | Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Alternative splicing and the use of alternative promoters results in multiple transcript variants. [provided by RefSeq, Oct 2016] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC228481L3 | Lenti-ORF clone of NOS3 (Myc-DDK-tagged)-Human nitric oxide synthase 3 (endothelial cell) (NOS3), transcript variant 2 |
CNY 6,460.00 |
|
RC228481L4 | Lenti-ORF clone of NOS3 (mGFP-tagged)-Human nitric oxide synthase 3 (endothelial cell) (NOS3), transcript variant 2 |
CNY 6,460.00 |
|
RG228481 | NOS3 (tGFP-tagged) - Human nitric oxide synthase 3 (endothelial cell) (NOS3), transcript variant 2 |
CNY 5,040.00 |
|
SC327116 | NOS3 (untagged)-Human nitric oxide synthase 3 (endothelial cell) (NOS3) transcript variant 2 |
CNY 4,448.00 |