Aminomethyltransferase (AMT) (NM_001164710) Human Tagged ORF Clone
CAT#: RC228289
- TrueORF®
AMT (Myc-DDK-tagged)-Human aminomethyltransferase (AMT), nuclear gene encoding mitochondrial protein, transcript variant 2
ORF Plasmid: tGFP
"NM_001164710" in other vectors (4)
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CNY 3,656.00
CNY 3,990.00
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | GCE; GCST; GCVT; NKH |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC228289 representing NM_001164710
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGAGGGCTGTAAGTGTGGTGGCCCGTCTGGGCTTTCGCCTGCAGGCATTCCCCCCGGCCTTGTGTC GTCCACTTAGTTGCGCACAGGAGGTGCTCCGCAGGACACCGCTCTATGACTTCCACCTGGCCCACGGCGG GAAAATGGTGGCGTTTGCGGGTTGGAGTCTGCCAGTGCAGTACCGGGACAGTCACACTGACTCGCACCTG CACACACGCCAGCACTGCTCGCTCTTTGACGTGTCTCATATGCTGCAGACCAAGATACTTGGTAGTGACC GGGTGAAGCTGATGGAGAGTCTAGTGGTTGGAGACATTGCAGAGCTAAGACCAAACCAGGACAAGGTCAG GGAGCTTCAGAACCAGGGCAGAGATGTGGGCCTGGAGGTGTTGGATAATGCCCTGCTAGCTCTGCAAGGC CCCACTGCAGCCCAGGTACTACAGGCCGGCGTGGCAGATGACCTGAGGAAACTGCCCTTCATGACCAGTG CTGTGATGGAGGTGTTTGGCGTGTCTGGCTGCCGCGTGACCCGCTGTGGCTACACAGGAGAGGATGGTGT GGAGATCTCGGTGCCGGTAGCGGGGGCAGTTCACCTGGCAACAGCTATTCTGAAAAACCCAGAGGTGAAG CTGGCAGGGCTGGCAGCCAGGGACAGCCTGCGCCTGGAGGCAGGCCTCTGCCTGTATGGGAATGACATTG ATGAACACACTACACCTGTGGAGGGCAGCCTCAGTTGGACACTGGGGAAGCGCCGCCGAGCTGCTATGGA CTTCCCTGGAGCCAAGGTCATTGTTCCCCAGCTGAAGGGCAGGGTGCAGCGGAGGCGTGTGGGGTTGATG TGTGAGGGGGCCCCCATGCGGGCACACAGTCCCATCCTGAACATGGAGGGTACCAAGATTGGTACTGTGA CTAGTGGCTGCCCCTCCCCCTCTCTGAAGAAGAATGTGGCGATGGGTTATGTGCCCTGCGAGTACAGTCG TCCAGGGACAATGCTGCTGGTAGAGGTGCGGCGGAAGCAGCAGATGGCTGTAGTCAGCAAGATGCCCTTT GTGCCCACAAACTACTATACCCTCAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC228289 representing NM_001164710
Red=Cloning site Green=Tags(s) MQRAVSVVARLGFRLQAFPPALCRPLSCAQEVLRRTPLYDFHLAHGGKMVAFAGWSLPVQYRDSHTDSHL HTRQHCSLFDVSHMLQTKILGSDRVKLMESLVVGDIAELRPNQDKVRELQNQGRDVGLEVLDNALLALQG PTAAQVLQAGVADDLRKLPFMTSAVMEVFGVSGCRVTRCGYTGEDGVEISVPVAGAVHLATAILKNPEVK LAGLAARDSLRLEAGLCLYGNDIDEHTTPVEGSLSWTLGKRRRAAMDFPGAKVIVPQLKGRVQRRRVGLM CEGAPMRAHSPILNMEGTKIGTVTSGCPSPSLKKNVAMGYVPCEYSRPGTMLLVEVRRKQQMAVVSKMPF VPTNYYTLK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001164710 |
ORF Size | 1077 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001164710.2 |
RefSeq ORF | 1080 bp |
Locus ID | 275 |
UniProt ID | P48728 |
Protein Pathways | Glycine, serine and threonine metabolism, Metabolic pathways, Nitrogen metabolism, One carbon pool by folate |
MW | 39.1 kDa |
Gene Summary | This gene encodes one of four critical components of the glycine cleavage system. Mutations in this gene have been associated with glycine encephalopathy. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC228289L3 | Lenti-ORF clone of AMT (Myc-DDK-tagged)-Human aminomethyltransferase (AMT), nuclear gene encoding mitochondrial protein, transcript variant 2 |
CNY 5,890.00 |
|
RC228289L4 | Lenti-ORF clone of AMT (mGFP-tagged)-Human aminomethyltransferase (AMT), nuclear gene encoding mitochondrial protein, transcript variant 2 |
CNY 5,890.00 |
|
RG228289 | AMT (tGFP-tagged) - Human aminomethyltransferase (AMT), nuclear gene encoding mitochondrial protein, transcript variant 2 |
CNY 4,370.00 |
|
SC326924 | AMT (untagged)-Human aminomethyltransferase (AMT) nuclear gene encoding mitochondrial protein transcript variant 2 |
CNY 5,800.00 |
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