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NELL2 (NM_001145110) Human Tagged ORF Clone
CAT＃: RC227981
NELL2 (Myc-DDK-tagged)-Human NEL-like 2 (chicken) (NELL2), transcript variant 5
ORF Plasmid: tGFP
"NM_001145110" in other vectors (4)
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CNY 6,144.00
CNY 8,840.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | NRP2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC227981 representing NM_001145110
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTATAAGAAGACTTCTCATTTTAATCCTCAAAATAGGGAGACGATGGACTGAGCTGATCCGCACCA TGGAGTCTCGGGTCTTACTGAGAACATTCTGTTTGATCTTCGGTCTCGGAGCAGTTTGGGGGCTTGGTGT GGACCCTTCCCTACAGATTGACGTCTTAACAGAGTTAGAACTTGGGGAGTCCACGACCGGAGTGCGTCAG GTCCCGGGGCTGCATAATGGGACGAAAGCCTTTCTCTTTCAAGATACTCCCAGAAGCATAAAAGCATCCA CTGCTACAGCTGAACAGTTTTTTCAGAAGCTGAGAAATAAACATGAATTTACTATTTTGGTGACCCTAAA ACAGACCCACTTAAATTCAGGAGTTATTCTCTCAATTCACCACTTGGATCACAGGTACCTGGAACTGGAA AGTAGTGGCCATCGGAATGAAGTCAGACTGCATTACCGCTCAGGCAGTCACCGCCCTCACACAGAAGTGT TTCCTTACATTTTGGCTGATGACAAGTGGCACAAGCTCTCCTTAGCCATCAGTGCTTCCCATTTGATTTT ACACATTGACTGCAATAAAATTTATGAAAGGGTAGTAGAAAAGCCCTCCACAGACTTGCCTCTAGGCACA ACATTTTGGCTAGGACAGAGAAATAATGCGCATGGATATTTTAAGGGTATAATGCAAGATGTCCAATTAC TTGTCATGCCCCAGGGATTTATTGCTCAGTGCCCAGATCTTAATCGCACCTGTCCAACTTGCAATGACTT CCATGGACTTGTGCAGAAAATCATGGAGCTACAGGATATTTTAGCCAAAACATCAGCCAAGCTGTCTCGA GCTGAACAGCGAATGAATAGATTGGATCAGTGCTATTGTGAAAGGACTTGCACCATGAAGGGAACCACCT ACCGAGAATTTGAGTCCTGGATAGACGGCTGTAAGAACTGCACATGCCTGAATGGAACCATCCAGTGTGA AACTCTAATCTGCCCAAATCCTGACTGCCCACTTAAGTCGGCTCTTGCGTATGTGGATGGCAAATGCTGT AAGGAATGCAAATCGATATGCCAATTTCAAGGACGAACCTACTTTGAAGGAGAAAGAAATACAGTCTATT CCTCTTCTGGAGTATGTGTTCTCTATGAGTGCAAGGACCAGACCATGAAACTTGTTGAGAGTTCAGGCTG TCCAGCTTTGGATTGTCCAGAGTCTCATCAGATAACCTTGTCTCACAGCTGTTGCAAAGTTTGTAAAGGT TATGACTTTTGTTCTGAAAGGCATAACTGCATGGAGAATTCCATCTGCAGAAATCTGAATGACAGGGCTG TTTGTAGCTGTCGAGATGGTTTTAGGGCTCTTCGAGAGGATAATGCCTACTGTGAAGACATCGATGAGTG TGCTGAAGGGCGCCATTACTGTCGTGAAAATACAATGTGTGTCAACACCCCGGGTTCTTTTATGTGCATC TGCAAAACTGGATACATCAGAATTGATGATTATTCATGTACAGAACATGATGAGTGTATCACAAATCAGC ACAACTGTGATGAAAATGCTTTATGCTTCAACACTGTTGGAGGACACAACTGTGTTTGCAAGCCGGGCTA TACAGGGAATGGAACGACATGCAAAGCATTTTGCAAAGATGGCTGTAGGAATGGAGGAGCCTGTATTGCC GCTAATGTGTGTGCCTGCCCACAAGGCTTCACTGGACCCAGCTGTGAAACGGACATTGATGAATGCTCTG ATGGTTTTGTTCAATGTGACAGTCGTGCTAATTGCATTAACCTGCCTGGATGGTACCACTGTGAGTGCAG AGATGGCTACCATGACAATGGGATGTTTTCACCAAGTGGAGAATCGTGTGAAGATATTGATGAGTGTGGG ACCGGGAGGCACAGCTGTGCCAATGATACCATTTGCTTCAATTTGGATGGCGGATATGATTGTCGATGTC CTCATGGAAAGAATTGCACAGGGGACTGCATCCATGATGGAAAAGTTAAGCACAATGGTCAGATTTGGGT GTTGGAAAATGACAGGTGCTCTGTGTGCTCATGTCAGAATGGATTCGTTATGTGTCGACGGATGGTCTGT GACTGTGAGAATCCCACAGTTGATCTTTTTTGCTGCCCTGAATGTGACCCAAGGCTTAGTAGTCAGTGCC TCCATCAAAATGGGGAAACTTTGTATAACAGTGGTGACACCTGGGTCCAGAATTGTCAACAGTGCCGCTG CTTGCAAGGGGAAGTTGATTGTTGGCCCCTGCCTTGCCCAGATGTGGAGTGTGAATTCAGCATTCTCCCA GAGAATGAGTGCTGCCCGCGCTGTGTCACAGACCCTTGCCAGGCTGACACCATCCGCAATGACATCACCA AGACTTGCCTGGACGAAATGAATGTGGTTCGCTTCACCGGGTCCTCTTGGATCAAACATGGCACTGAGTG TACTCTCTGCCAGTGCAAGAATGGCCACATCTGTTGCTCAGTGGATCCACAGTGCCTTCAGGAACTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC227981 representing NM_001145110
Red=Cloning site Green=Tags(s) MSIRRLLILILKIGRRWTELIRTMESRVLLRTFCLIFGLGAVWGLGVDPSLQIDVLTELELGESTTGVRQ VPGLHNGTKAFLFQDTPRSIKASTATAEQFFQKLRNKHEFTILVTLKQTHLNSGVILSIHHLDHRYLELE SSGHRNEVRLHYRSGSHRPHTEVFPYILADDKWHKLSLAISASHLILHIDCNKIYERVVEKPSTDLPLGT TFWLGQRNNAHGYFKGIMQDVQLLVMPQGFIAQCPDLNRTCPTCNDFHGLVQKIMELQDILAKTSAKLSR AEQRMNRLDQCYCERTCTMKGTTYREFESWIDGCKNCTCLNGTIQCETLICPNPDCPLKSALAYVDGKCC KECKSICQFQGRTYFEGERNTVYSSSGVCVLYECKDQTMKLVESSGCPALDCPESHQITLSHSCCKVCKG YDFCSERHNCMENSICRNLNDRAVCSCRDGFRALREDNAYCEDIDECAEGRHYCRENTMCVNTPGSFMCI CKTGYIRIDDYSCTEHDECITNQHNCDENALCFNTVGGHNCVCKPGYTGNGTTCKAFCKDGCRNGGACIA ANVCACPQGFTGPSCETDIDECSDGFVQCDSRANCINLPGWYHCECRDGYHDNGMFSPSGESCEDIDECG TGRHSCANDTICFNLDGGYDCRCPHGKNCTGDCIHDGKVKHNGQIWVLENDRCSVCSCQNGFVMCRRMVC DCENPTVDLFCCPECDPRLSSQCLHQNGETLYNSGDTWVQNCQQCRCLQGEVDCWPLPCPDVECEFSILP ENECCPRCVTDPCQADTIRNDITKTCLDEMNVVRFTGSSWIKHGTECTLCQCKNGHICCSVDPQCLQEL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001145110 |
| ORF Size | 2517 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001145110.1, NP_001138582.1 |
| RefSeq ORF | 2520 bp |
| Locus ID | 4753 |
| UniProt ID | Q99435 |
| Protein Families | Secreted Protein, Transmembrane |
| MW | 94 kDa |
| Gene Summary | The protein encoded by this gene is a glycoprotein containing several von Willebrand factor C domains and epidermal growth factor (EGF)-like domains. The encoded protein acts as a homotrimer and is found in the cytoplasm. Several variants encoding a few different isoforms exist, and at least one isoform appears to be a secreted protein. Studies in mouse suggest that this protein plays a role in neural cell growth and differentiation as well as in oncogenesis. [provided by RefSeq, Feb 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC227981L3 | Lenti ORF clone of Human NEL-like 2 (chicken) (NELL2), transcript variant 5, Myc-DDK-tagged |
CNY 10,740.00 |
|
| RC227981L4 | Lenti ORF clone of Human NEL-like 2 (chicken) (NELL2), transcript variant 5, mGFP tagged |
CNY 10,740.00 |
|
| RG227981 | NELL2 (tGFP-tagged) - Human NEL-like 2 (chicken) (NELL2), transcript variant 5 |
CNY 9,690.00 |
|
| SC326252 | NELL2 (untagged)-Human NEL-like 2 (chicken) (NELL2), transcript variant 5 |
CNY 16,250.00 |
