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ATF6 beta (ATF6B) (NM_001136153) Human Tagged ORF Clone
CAT＃: RC227970
- TrueORF®
ATF6B (Myc-DDK-tagged)-Human activating transcription factor 6 beta (ATF6B), transcript variant 2
ORF Plasmid: tGFP
"NM_001136153" in other vectors (4)
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CNY 5,128.00
CNY 5,800.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CREB-RP; CREBL1; G13 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC227970 representing NM_001136153
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGAGCTGATGCTGCTCAGCGAGATTGCTGACCCGACGCGTTTCTTCACCGACAACCTGCTTAGCC CGGAGGACTGGGACAGCACCTTGTATTCTGGCCTAGATGAAGTGGCCGAGGAGCAGACGCAGCTCTTCCG TTGCCCGGAGCAGGATGTCCCGTTTGACGGCAGCTCCCTGGACGTGGGGATGGATGTCAGCCCCTCTGAG CCCCCATGGGAACTCCTGCCGATCTTCCCAGATCTTCAGGTGAAGTCTGAGCCATCTTCCCCCTGCTCTT CCTCCTCCCTCAGCTCCGAGTCATCGCGTCTCTCCACAGAGCCATCCAGCGAGGCTCTTGGGGTAGGGGA GGTGCTCCATGTGAAGACAGAGTCCTTGGCACCCCCACTGTGTCTCCTGGGAGATGACCCAACATCCTCA TTTGAAACCGTCCAGATCAACGTTATCCCCACCTCTGATGATTCCTCAGATGTCCAGACCAAGATAGAAC CTGTCTCTCCATGTTCTTCCGTCAACTCTGAGGCCTCCCTGCTCTCAGCCGACTCCTCCAGCCAGGCTTT TATAGGAGAGGAGGTCCTGGAAGTGAAGACAGAGTCCCTGTCCCCTTCAGGATGCCTCCTGTGGGATGTC CCAGCCCCCTCACTTGGAGCTGTCCAGATCAGCATGGGCCCATCCCTTGATGGCTCCTCAGGCAAAGCCC TGCCCACCCGGAAGCCGCCACTGCAGCCCAAACCTGTAGTGCTAACCACTGTCCCAATGCCATCCAGAGC TGTGCCTCCCAGCACCACAGTCCTTCTGCAGTCCCTCGTCCAGCCACCCCCAGTGTCCCCAGTTGTCCTC ATCCAGGGTGCTATTCGAGTCCAGCCTGAAGGGCCGGCTCCCTCTCTACCACGGCCTGAGAGGAAGAGCA TCGTTCCCGCTCCTATGCCTGGAAACTCCTGCCCGCCTGAAGTGGATGCAAAGCTGCTGAAGCGGCAGCA GCGAATGATCAAGAACCGGGAGTCAGCCTGCCAGTCCCGGAGAAAGAAGAAAGAGTATCTGCAGGGACTG GAGGCTCGGCTGCAAGCAGTACTGGCTGACAACCAGCAGCTCCGCCGAGAGAATGCTGCCCTCCGGCGGC GGCTGGAGGCCCTGCTGGCTGAAAACAGCGAGCTCAAGTTAGGGTCTGGAAACAGGAAGGTGGTCTGCAT CATGGTCTTCCTTCTCTTCATTGCCTTCAACTTTGGACCTGTCAGCATCAGTGAGCCTCCTTCAGCTCCC ATCTCTCCTCGGATGAACAAGGGGGAGCCTCAACCCCGGAGACACTTGCTGGGGTTCTCAGAGCAAGAGC CAGTTCAGGGAGTTGAACCTCTCCAGGGGTCCTCCCAGGGCCCTAAGGAGCCCCAGCCCAGCCCCACAGA CCAGCCCAGTTTCAGCAACCTGACAGCCTTCCCTGGGGGCGCCAAGGAGCTACTACTAAGAGACCTAGAC CAGCTCTTCCTCTCCTCTGATTGCCGGCACTTCAACCGCACTGAGTCCCTGAGGCTTGCTGACGAGTTGA GTGGCTGGGTCCAGCGCCACCAGAGAGGCCGGAGGAAGATCCCTCAGAGGGCCCAGGAGAGACAGAAGTC TCAGCCACGGAAGAAGTCACCTCCAGTTAAGGCAGTCCCCATCCAACCCCCTGGACCCCCAGAAAGGGAT TCTGTGGGCCAGCTGCAACTATATCGCCACCCAGACCGTTCGCAGCCAGCATTCTTGGATGCAATTGACC GACGGGAAGACACATTTTATGTTGTCTCTTTCCGAAGGGACCACCTGCTGCTCCCAGCCATCAGCCACAA CAAGACCTCCCGGCCCAAGATGTCCCTGGTGATGCCTGCCATGGCCCCCAATGAGACCCTGTCAGGCCGT GGGGCCCCGGGGGACTATGAGGAGATGATGCAGATCGAGTGTGAGGTCATGGACACCAGGGTGATTCACA TCAAGACCTCCACAGTGCCCCCCTCGCTCCGAAAACAGCCATCCCCAACCCCAGGCAATGCCACAGGTGG CCCCTTGCCAGTCTCTGCAGCCAGCCAGGCCCACCAGGCCTCCCACCAGCCCCTCTACCTCAATCATCCC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >RC227970 representing NM_001136153
Red=Cloning site Green=Tags(s) MAELMLLSEIADPTRFFTDNLLSPEDWDSTLYSGLDEVAEEQTQLFRCPEQDVPFDGSSLDVGMDVSPSE PPWELLPIFPDLQVKSEPSSPCSSSSLSSESSRLSTEPSSEALGVGEVLHVKTESLAPPLCLLGDDPTSS FETVQINVIPTSDDSSDVQTKIEPVSPCSSVNSEASLLSADSSSQAFIGEEVLEVKTESLSPSGCLLWDV PAPSLGAVQISMGPSLDGSSGKALPTRKPPLQPKPVVLTTVPMPSRAVPPSTTVLLQSLVQPPPVSPVVL IQGAIRVQPEGPAPSLPRPERKSIVPAPMPGNSCPPEVDAKLLKRQQRMIKNRESACQSRRKKKEYLQGL EARLQAVLADNQQLRRENAALRRRLEALLAENSELKLGSGNRKVVCIMVFLLFIAFNFGPVSISEPPSAP ISPRMNKGEPQPRRHLLGFSEQEPVQGVEPLQGSSQGPKEPQPSPTDQPSFSNLTAFPGGAKELLLRDLD QLFLSSDCRHFNRTESLRLADELSGWVQRHQRGRRKIPQRAQERQKSQPRKKSPPVKAVPIQPPGPPERD SVGQLQLYRHPDRSQPAFLDAIDRREDTFYVVSFRRDHLLLPAISHNKTSRPKMSLVMPAMAPNETLSGR GAPGDYEEMMQIECEVMDTRVIHIKTSTVPPSLRKQPSPTPGNATGGPLPVSAASQAHQASHQPLYLNHP SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001136153 |
| ORF Size | 2100 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001136153.2 |
| RefSeq ORF | 2103 bp |
| Locus ID | 1388 |
| UniProt ID | Q99941 |
| Protein Families | Transcription Factors |
| MW | 76.2 kDa |
| Gene Summary | The protein encoded by this gene is a transcription factor in the unfolded protein response (UPR) pathway during ER stress. Either as a homodimer or as a heterodimer with ATF6-alpha, the encoded protein binds to the ER stress response element, interacting with nuclear transcription factor Y to activate UPR target genes. The protein is normally found in the membrane of the endoplasmic reticulum; however, under ER stress, the N-terminal cytoplasmic domain is cleaved from the rest of the protein and translocates to the nucleus. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC227970L3 | Lenti ORF clone of Human activating transcription factor 6 beta (ATF6B), transcript variant 2, Myc-DDK-tagged |
CNY 7,700.00 |
|
| RC227970L4 | Lenti ORF clone of Human activating transcription factor 6 beta (ATF6B), transcript variant 2, mGFP tagged |
CNY 7,700.00 |
|
| RG227970 | ATF6B (tGFP-tagged) - Human activating transcription factor 6 beta (ATF6B), transcript variant 2 |
CNY 6,370.00 |
|
| SC326184 | ATF6B (untagged)-Human activating transcription factor 6 beta (ATF6B), transcript variant 2 |
CNY 13,490.00 |
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