ZNF534 (NM_001143939) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	KRBO3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC227638 ORF sequence, codon optimized. Due to the complexity of NM_001143939, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTCTCACGCAAGGCCAGTTGTCCTTCTCCGATGTCGCCATCGAGTTCTCACAGGAGGAATGGAAGT GTTTGGATCCCGGGCAGAAGGCCCTTTATCGGGACGTTATGCTCGAAAACTACCGGAACCTCGTGAGCCT GGGGGAGGACAACGTACGGCCCGAGGCCTGCATTTGTTCCGGCATCTGCCTCCCCGACTTGTCTGTGACA AGCATGCTGGAGCAGAAACGCGACCCTTGGACCCTGCAGTCTGAGGTCAAGATTATTAACAATCCTGATG GTCGGGAATGCATCAAAGGAGTCAATACCGAAAAGTCTAGCAAACTTGGGTCTTCAGCTGGAAATAAATC CCTGAAAAATCAGCACGGTTTGACCTTGCAGCTGCACCTGACGGAGTGGCAACCATTTCAGGCGGTTCGG AACATCTACGGTTGCAAACATGTGGAGAAGTCAATCAGTGACAACTCATCCGTGTCTCCTGTGCAGATTA GCTTTTTTAGTGTGAAAACTCACATCTTCAATAACTACAGGAACGACTTCCTCTTCAGTACCCTCCTGCC ACAGGAGCAGAAAGTGCACATCCGCGAGAAACCTTACGGCTGTAACGAGCATGGCAAGGTATTCCGGGTG AGCTCTTCCCTCACAAACCGGCAAGTTATCCACATCGCCGACAAGACCTATAAGTGTTCAGACTGCGGGG AAATTTTCAGCAGTAACTCCAATTTTGCCCAGCACCAGAGGATCCATACGGGCGAGAAACCTTACAAGTA CAACGAGTGTGGCAAGGTGTTTAATCAAAATTCACATCTGGCTCAGCATCAAAAGATTCATACAGGCCAG AAGCCTTATAATAACAAAGAATGTGGCAAGGTCTTCTCCCACCACGCCTATCTGGCACAACACCGGAAGA TACATACAGGCGAGAAACCTTACAAGTGTTCCGAATGTGGCAAGGCTTTTTCTGTGTGCTCATCATTGAC CGCCCATCTTGTGATTCACACCGGAGAAAAGCCCTATGATTGCAAAGAGTGCGGGAAAGTGTTTAGGCAC AAATCCAGCCTGACAACTCATCAGACGGTGCATACTGGCGAAAGACCCTACAAATGCAACGAATGTGGTA AAGGCTTCTCCAGAATTGCATTTCTTGCCCGCCACCGAAAAGTTCACACTGGTGAGAAGCCATATAAATG TAACGAATGCGGCAAAGTCTTTATAGGAAATAGCCGACTGGCCAGACACCGAAAGATACATACAGGAGGT AGAAGGTACAAATGTAACGAATGTGGCAAGGCCTTTAGAACGTGCAGCGACCTCACTGCTCATCTCCTGA TCCACACCGGTGAAAAACCGTATGAGTGTATAGATTGTGGAAAGGTATTCAGACACAAATCATCTCTGAC CTACCATTGCAGGATTCACACCGGGGAGAAGCCTTATAAGTGTAACGAATGCGGGAAAGTATTTAGCCAG AACAGCAACCTTCAGCGGCACCGGAAAATCCACACCGGTGAAAAACTCTATAAGTGCAACGAATGCGGGA AGGTGTTCCGGCAGAACAGCCATCTGGCCCAACACAGAGACATTCACACTGGCGAAAAGCCCTATAGCTG CAACGAATGCGGGAAGGTCTTCCGGAGGAATAGTCACTTGGTGCGACACAGGAACGTCCATACCGGGGAA AAACCCTATTCCTGCAACGAATGCGGTAAGGTGTTCTCCCGCAACAGCCATCTCGCCAGACACCGCAATA TCCACACCGGCGAAAAACCTCATTCCTGTAACGAGTGTGGCAAAGTATTTAGCAGGAATAGCCACCTGGC TAGACATCGCAAGATTCATACTGGAGAGAAGCTCTACAAATGCAACGAGTGCTCAAAAGTCTTTAGTCGC AACTCACGCCTGGCTCAGCACAGAAACATCCACACGGGGGTGAAGCCTTATAGCTGTAACGAGTGCGGGA AGGTATTCTCCAAGAATTCCATCCTTGTGCAACACTGTAGCATTCATACACGAGAGAAACCG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC227638 representing NM_001143939 Red=Cloning site Green=Tags(s) MALTQGQLSFSDVAIEFSQEEWKCLDPGQKALYRDVMLENYRNLVSLGEDNVRPEACICSGICLPDLSVT SMLEQKRDPWTLQSEVKIINNPDGRECIKGVNTEKSSKLGSSAGNKSLKNQHGLTLQLHLTEWQPFQAVR NIYGCKHVEKSISDNSSVSPVQISFFSVKTHIFNNYRNDFLFSTLLPQEQKVHIREKPYGCNEHGKVFRV SSSLTNRQVIHIADKTYKCSDCGEIFSSNSNFAQHQRIHTGEKPYKYNECGKVFNQNSHLAQHQKIHTGQ KPYNNKECGKVFSHHAYLAQHRKIHTGEKPYKCSECGKAFSVCSSLTAHLVIHTGEKPYDCKECGKVFRH KSSLTTHQTVHTGERPYKCNECGKGFSRIAFLARHRKVHTGEKPYKCNECGKVFIGNSRLARHRKIHTGG RRYKCNECGKAFRTCSDLTAHLLIHTGEKPYECIDCGKVFRHKSSLTYHCRIHTGEKPYKCNECGKVFSQ NSNLQRHRKIHTGEKLYKCNECGKVFRQNSHLAQHRDIHTGEKPYSCNECGKVFRRNSHLVRHRNVHTGE KPYSCNECGKVFSRNSHLARHRNIHTGEKPHSCNECGKVFSRNSHLARHRKIHTGEKLYKCNECSKVFSR NSRLAQHRNIHTGVKPYSCNECGKVFSKNSILVQHCSIHTREKP TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001143939
ORF Size	2022 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001143939.1, NP_001137411.1
RefSeq Size	5425 bp
RefSeq ORF	2025 bp
Locus ID	147658
UniProt ID	Q76KX8
MW	77.2 kDa
Gene Summary	May be involved in transcriptional regulation.[UniProtKB/Swiss-Prot Function]

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