URM1 (NM_001135947) Human Tagged Lenti ORF Clone
CAT#: RC227597L4
- LentiORF®
Lenti-ORF clone of URM1 (mGFP-tagged)-Human ubiquitin related modifier 1 (URM1), transcript variant 2
"NM_001135947" in other vectors (4)
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CNY 5,890.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | mGFP |
Synonyms | C9orf74 |
Vector | pLenti-C-mGFP-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(RC227597).
|
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001135947 |
ORF Size | 438 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001135947.2, NP_001129419.1 |
RefSeq Size | 3006 bp |
RefSeq ORF | 441 bp |
Locus ID | 81605 |
UniProt ID | Q9BTM9 |
MW | 15.8 kDa |
Gene Summary | Acts as a sulfur carrier required for 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Serves as sulfur donor in tRNA 2-thiolation reaction by being thiocarboxylated (-COSH) at its C-terminus by MOCS3. The sulfur is then transferred to tRNA to form 2-thiolation of mcm(5)S(2)U. Also acts as a ubiquitin-like protein (UBL) that is covalently conjugated via an isopeptide bond to lysine residues of target proteins such as MOCS3, ATPBD3, CTU2, USP15 and CAS. The thiocarboxylated form serves as substrate for conjugation and oxidative stress specifically induces the formation of UBL-protein conjugates.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC227597 | URM1 (Myc-DDK-tagged)-Human ubiquitin related modifier 1 (URM1), transcript variant 2 |
CNY 3,990.00 |
|
RC227597L3 | Lenti-ORF clone of URM1 (Myc-DDK-tagged)-Human ubiquitin related modifier 1 (URM1), transcript variant 2 |
CNY 5,890.00 |
|
RG227597 | URM1 (tGFP-tagged) - Human ubiquitin related modifier 1 (URM1), transcript variant 2 |
CNY 4,370.00 |
|
SC325517 | URM1 (untagged)-Human ubiquitin related modifier 1 (URM1), transcript variant 2 |
CNY 3,990.00 |