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Myosin Phosphatase (PPP1R12A) (NM_001143885) Human Tagged ORF Clone
CAT＃: RC227493
PPP1R12A (Myc-DDK-tagged)-Human protein phosphatase 1, regulatory (inhibitor) subunit 12A (PPP1R12A), transcript variant 2
ORF Plasmid: tGFP
"NM_001143885" in other vectors (6)
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CNY 7,176.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | GUBS; M130; MBS; MYPT1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC227493 representing NM_001143885
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGATGGCGGACGCGAAGCAGAAGCGGAACGAGCAGCTGAAACGCTGGATCGGCTCCGAGACGGACC TCGAGCCTCCGGTGGTGAAGCGCCAGAAGACCAAGGTGAAGTTCGACGATGGCGCCGTCTTCCTGGCTGC TTGCTCCAGCGGCGACACGGACGAGGTCCTCAAGCTGCTGCACCGCGGCGCCGACATCAATTACGCCAAT GTGGACGGACTCACTGCCCTGCACCAGGCTTGCATTGATGACAATGTTGATATGGTGAAGTTTCTGGTAG AAAATGGAGCAAATATTAATCAACCTGATAATGAAGGCTGGATACCACTACATGCAGCAGCTTCCTGTGG ATATCTTGATATTGCAGAGTTTTTGATTGGTCAAGGAGCACATGTAGGGGCTGTCAACAGTGAAGGAGAT ACACCTTTAGATATTGCGGAGGAGGAGGCAATGGAAGAGCTACTTCAAAATGAAGTTAATCGGCAAGGGG TTGATATAGAAGCAGCTCGAAAGGAAGAAGAACGGATCATGCTTAGAGATGCCAGGCAGTGGCTAAATAG TGGTCATATAAATGATGTCCGGCATGCAAAATCTGGAGGTACAGCACTTCACGTTGCAGCTGCTAAAGGC TATACGGAAGTTTTAAAACTTTTAATACAGGCAGGCTATGATGTTAATATTAAAGACTATGATGGCTGGA CACCTCTTCATGCTGCAGCTCATTGGGGTAAAGAAGAAGCATGTCGAATTTTAGTGGACAATCTGTGTGA TATGGAGATGGTCAACAAAGTGGGCCAAACAGCCTTTGATGTAGCAGATGAAGACATTTTAGGATATTTA GAAGAGTTGCAAAAGAAACAAAATCTGCTCCATAGTGAAAAACGGGACAAGAAATCTCCACTAATTGAAT CAACAGCAAATATGGACAATAATCAGTCACAGAAGACCTTTAAAAACAAAGAGACGTTGATTATTGAACC AGAGAAAAATGCATCCCGTATTGAATCTCTGGAACAAGAAAAGGTTGATGAAGAAGAAGAAGGAAAGAAG GATGAGTCTAGCTGCTCTAGTGAAGAAGATGAGGAAGATGACTCGGAATCAGAAGCTGAAACAGATAAGA CAAAACCCCTGGCTTCTGTAACTAATGCCAACACTTCTAGTACACAAGCAGCTCCTGTAGCTGTTACAAC ACCTACTGTGTCATCAGGTCAAGCAACACCTACATCACCTATTAAAAAGTTTCCAACCACAGCTACAAAA ATTTCTCCCAAAGAAGAAGAGAGAAAAGATGAGTCTCCTGCAACTTGGAGGTTAGGACTTAGAAAGACGG GCAGCTATGGTGCACTTGCTGAAATCACAGCATCTAAAGAGGGTCAGAAAGAAAAAGATACTGCAGGTGT TACACGTTCAGCTTCAAGTCCCAGACTTTCCTCCTCTTTGGATAATAAAGAAAAGGAGAAAGATAGTAAA GGAACTAGGCTTGCATATGTTGCACCTACAATACCAAGACGACTAGCCAGTACATCTGACATTGAAGAGA AAGAAAACAGAGATTCTTCAAGTTTGCGAACAAGTAGTTCATATACAAGGAGAAAATGGGAAGATGATCT TAAAAAAAATAGCTCAGTTAATGAAGGATCAACGTATCATAAAAGTTGCTCCTTTGGTAGAAGACAAGAT GATTTGATTAGTTCTAGTGTTCCAAGCACCACATCAACACCAACAGTTACCTCTGCAGCTGGGCTTCAGA AAAGCCTGCTTTCCAGCACAAGCACTACTACAAAGATTACAACGGGTTCTTCCTCAGCAGGCACACAAAG CAGTACCTCAAATCGTTTGTGGGCTGAGGATAGTACTGAGAAAGAAAAGGACAGTGTTCCTACGGCAGTG ACCATTCCTGTTGCTCCAACTGTTGTAAATGCTGCAGCTTCTACCACAACCCTGACTACAACTACTGCTG GCACTGTCTCCTCCACAACAGAGGTCAGGGAGAGACGCAGATCATACCTCACTCCTGTTAGGGATGAAGA GTCTGAATCCCAAAGAAAAGCAAGATCTAGACAAGCAAGACAATCTAGAAGATCAACACAGGGAGTGACA TTAACTGATCTTCAAGAAGCTGAGAAAACAATAGGAAGAAGTCGTTCTACCCGAACCAGAGAACAAGAAA ATGAAGAAAAAGAAAAAGAGGAAAAAGAGAAACAAGATAAAGAGAAACAAGAAGAAAAGAAGGAGTCAGA AACATCTAGAGAAGATGAATATAAACAAAAGTACTCCAGAACGTATGATGAGACTTACCAGCGTTATAGG CCAGTATCAACTTCAAGTTCAACCACTCCATCCTCTTCACTTTCTACTATGAGCAGTTCACTGTATGCTT CAAGTCAACTAAACAGGCCAAATAGTCTTGTAGGCATAACTTCTGCTTACTCCAGAGGAATAACAAAAGA AAATGAAAGAGAGGGAGAAAAAAGAGAAGAGGAGAAAGAAGGAGAAGATAAATCACAACCTAAATCAATC AGAGAACGACGACGACCAAGAGAGAAAAGAAGATCTACAGGAGTTTCATTTTGGACACAAGATAGTGATG AAAATGAACAAGAACAACAATCAGACACAGAAGAGGGATCCAATAAGAAAGAAACTCAGACGGATTCCAT TTCTAGATATGAAACCAGTTCTACATCAGCTGGTGATCGATATGATTCCTTGCTGGGTCGCTCTGGATCA TACAGTTACTTAGAAGAAAGAAAACCTTACAGCAGCAGGCTAGAAAAGGATGACTCAACTGACTTTAAAA AGCTTTATGAACAAATTCTAGCTGAAAATGAAAAGCTGAAGGCACAGCTACATGATACAAATATGGAACT AACAGATCTTAAATTACAGTTGGAAAAGGCCACCCAGAGACAAGAAAGATTTGCTGATAGATCACTGTTG GAAATGGAAAAAAGGGAACGAAGAGCTCTAGAAAGAAGAATATCTGAAATGGAAGAAGAGCTCAAAATGT TACCAGACCTAAAAGCAGACAACCAGAGGCTAAAGGATGAAAATGGGGCCTTGATCAGAGTTATAAGCAA ACTTTCCAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC227493 representing NM_001143885
Red=Cloning site Green=Tags(s) MKMADAKQKRNEQLKRWIGSETDLEPPVVKRQKTKVKFDDGAVFLAACSSGDTDEVLKLLHRGADINYAN VDGLTALHQACIDDNVDMVKFLVENGANINQPDNEGWIPLHAAASCGYLDIAEFLIGQGAHVGAVNSEGD TPLDIAEEEAMEELLQNEVNRQGVDIEAARKEEERIMLRDARQWLNSGHINDVRHAKSGGTALHVAAAKG YTEVLKLLIQAGYDVNIKDYDGWTPLHAAAHWGKEEACRILVDNLCDMEMVNKVGQTAFDVADEDILGYL EELQKKQNLLHSEKRDKKSPLIESTANMDNNQSQKTFKNKETLIIEPEKNASRIESLEQEKVDEEEEGKK DESSCSSEEDEEDDSESEAETDKTKPLASVTNANTSSTQAAPVAVTTPTVSSGQATPTSPIKKFPTTATK ISPKEEERKDESPATWRLGLRKTGSYGALAEITASKEGQKEKDTAGVTRSASSPRLSSSLDNKEKEKDSK GTRLAYVAPTIPRRLASTSDIEEKENRDSSSLRTSSSYTRRKWEDDLKKNSSVNEGSTYHKSCSFGRRQD DLISSSVPSTTSTPTVTSAAGLQKSLLSSTSTTTKITTGSSSAGTQSSTSNRLWAEDSTEKEKDSVPTAV TIPVAPTVVNAAASTTTLTTTTAGTVSSTTEVRERRRSYLTPVRDEESESQRKARSRQARQSRRSTQGVT LTDLQEAEKTIGRSRSTRTREQENEEKEKEEKEKQDKEKQEEKKESETSREDEYKQKYSRTYDETYQRYR PVSTSSSTTPSSSLSTMSSSLYASSQLNRPNSLVGITSAYSRGITKENEREGEKREEEKEGEDKSQPKSI RERRRPREKRRSTGVSFWTQDSDENEQEQQSDTEEGSNKKETQTDSISRYETSSTSAGDRYDSLLGRSGS YSYLEERKPYSSRLEKDDSTDFKKLYEQILAENEKLKAQLHDTNMELTDLKLQLEKATQRQERFADRSLL EMEKRERRALERRISEMEEELKMLPDLKADNQRLKDENGALIRVISKLSK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001143885 |
| ORF Size | 3090 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001143885.1, NP_001137357.1 |
| RefSeq ORF | 3093 bp |
| Locus ID | 4659 |
| UniProt ID | O14974 |
| Protein Families | Druggable Genome |
| Protein Pathways | Focal adhesion, Long-term potentiation, Regulation of actin cytoskeleton, Vascular smooth muscle contraction |
| MW | 115.1 kDa |
| Gene Summary | Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC227493L1 | Lenti ORF clone of Human protein phosphatase 1, regulatory (inhibitor) subunit 12A (PPP1R12A), transcript variant 2, Myc-DDK-tagged |
CNY 10,260.00 |
|
| RC227493L2 | Lenti ORF clone of Human protein phosphatase 1, regulatory (inhibitor) subunit 12A (PPP1R12A), transcript variant 2, mGFP tagged |
CNY 10,260.00 |
|
| RC227493L3 | Lenti ORF clone of Human protein phosphatase 1, regulatory (inhibitor) subunit 12A (PPP1R12A), transcript variant 2, Myc-DDK-tagged |
CNY 10,260.00 |
|
| RC227493L4 | Lenti ORF clone of Human protein phosphatase 1, regulatory (inhibitor) subunit 12A (PPP1R12A), transcript variant 2, mGFP tagged |
CNY 10,260.00 |
|
| RG227493 | PPP1R12A (tGFP-tagged) - Human protein phosphatase 1, regulatory (inhibitor) subunit 12A (PPP1R12A), transcript variant 2 |
CNY 9,220.00 |
|
| SC326318 | PPP1R12A (untagged)-Human protein phosphatase 1, regulatory (inhibitor) subunit 12A (PPP1R12A), transcript variant 2 |
CNY 19,950.00 |
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