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SEC13L1 (SEC13) (NM_001136232) Human Tagged ORF Clone
CAT＃: RC226933
SEC13 (Myc-DDK-tagged)-Human SEC13 homolog (S. cerevisiae) (SEC13), transcript variant 2
ORF Plasmid: tGFP
"NM_001136232" in other vectors (4)
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CNY 2,400.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | D3S1231E; npp-20; SEC13L1; SEC13R |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC226933 representing NM_001136232
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATTCACGACGCCCAGATGGACTACTATGGCACCCGCCTGGCAACCTGCTCATCAGACAGGTCCGTCA AAATCTTTGATGTGCGCAATGGAGGGCAGATCCTTATCGCCGACCTCAGGGGTCATGAGGGTCCTGTGTG GCAAGTGGCCTGGGCTCACCCCATGTACGGCAACATCCTGGCATCGTGCTCCTATGACCGGAAAGTCATT ATCTGGAGAGAGGAAAACGGCACCTGGGAGAAGAGCCACGAGCATGCGGGACACGACTCCTCAGTGAACT CGGTGTGCTGGGCCCCCCATGACTACGGCCTGATCCTGGCCTGTGGGAGCTCGGATGGGGCCATCTCCCT GCTGACTTACACCGGGGAAGGCCAATGGGAAGTAAAGAAGATCAACAACGCTCACACCATTGGCTGCAAT GCCGTCAGCTGGGCCCCTGCTGTTGTACCTGGAAGCCTCATAGACCACCCATCGGGGCAGAAACCCAATT ACATCAAGAGGTTTGCATCAGGTGGCTGTGACAACCTCATCAAGCTGTGGAAGGAGGAGGAGGACGGCCA GTGGAAGGAGGAGCAGAAGCTAGAAGCGCACAGTGACTGGGTTCGAGATGTGGCCTGGGCCCCCTCCATC GGCCTGCCCACCAGCACCATCGCCAGCTGCTCCCAGGATGGTCGTGTGTTCATTTGGACCTGTGATGATG CCTCAAGCAATACGTGGTCCCCTAAATTGTTGCACAAGTTCAACGATGTGGTGTGGCATGTGAGCTGGTC CATCACAGCCAACATCCTGGCTGTCTCTGGTGGAGACAATAAGGTGACCCTGTGGAAGGAGTCAGTTGAT GGGCAGTGGGTGTGCATCAGTGATGTCAACAAGGGCCAGGGCTCCGTATCAGCATCAGTGACAGAGGGCC AGCAGAACGAGCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC226933 representing NM_001136232
Red=Cloning site Green=Tags(s) MIHDAQMDYYGTRLATCSSDRSVKIFDVRNGGQILIADLRGHEGPVWQVAWAHPMYGNILASCSYDRKVI IWREENGTWEKSHEHAGHDSSVNSVCWAPHDYGLILACGSSDGAISLLTYTGEGQWEVKKINNAHTIGCN AVSWAPAVVPGSLIDHPSGQKPNYIKRFASGGCDNLIKLWKEEEDGQWKEEQKLEAHSDWVRDVAWAPSI GLPTSTIASCSQDGRVFIWTCDDASSNTWSPKLLHKFNDVVWHVSWSITANILAVSGGDNKVTLWKESVD GQWVCISDVNKGQGSVSASVTEGQQNEQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001136232 |
| ORF Size | 924 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001136232.2, NP_001129704.1 |
| RefSeq ORF | 927 bp |
| Locus ID | 6396 |
| UniProt ID | P55735 |
| MW | 33.8 kDa |
| Gene Summary | The protein encoded by this gene belongs to the SEC13 family of WD-repeat proteins. It is a constituent of the endoplasmic reticulum and the nuclear pore complex. It has similarity to the yeast SEC13 protein, which is required for vesicle biogenesis from endoplasmic reticulum during the transport of proteins. Multiple alternatively spliced transcript variants have been found. [provided by RefSeq, Oct 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC226933L3 | Lenti ORF clone of Human SEC13 homolog (S. cerevisiae) (SEC13), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC226933L4 | Lenti ORF clone of Human SEC13 homolog (S. cerevisiae) (SEC13), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG226933 | SEC13 (tGFP-tagged) - Human SEC13 homolog (S. cerevisiae) (SEC13), transcript variant 2 |
CNY 4,000.00 |
|
| SC324922 | SEC13 (untagged)-Human SEC13 homolog (S. cerevisiae) (SEC13), transcript variant 2 |
CNY 6,270.00 |
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