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LYAR (NM_001145725) Human Tagged ORF Clone
CAT＃: RC226844
LYAR (Myc-DDK-tagged)-Human Ly1 antibody reactive homolog (mouse) (LYAR), transcript variant 2
ORF Plasmid: tGFP
"NM_001145725" in other vectors (4)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ZC2HC2; ZLYAR |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC226844 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTATTTTTTACATGCAATGCATGTGGTGAATCAGTGAAGAAAATACAAGTGGAAAAGCATGTGTCTG TTTGCAGAAACTGTGAATGCCTTTCTTGCATTGACTGCGGTAAAGATTTCTGGGGCGATGACTATAAAAA CCACGTGAAATGCATAAGTGAAGATCAGAAGTATGGTGGCAAAGGCTATGAAGGTAAAACCCACAAAGGC GACATCAAACAGCAGGCGTGGATTCAGAAAATTAGTGAATTAATAAAGAGACCCAATGTCAGCCCCAAAG TGAGAGAACTTTTAGAGCAAATTAGTGCTTTTGACAACGTTCCCAGGAAAAAGGCAAAATTTCAGAATTG GATGAAGAACAGTTTAAAAGTTCATAATGAATCCATTCTGGACCAGGTGTGGAATATCTTTTCTGAAGCT TCCAACAGCGAACCAGTCAATAAGGAACAGGATCAACGGCCACTCCACCCAGTGGCAAATCCACATGCAG AAATCTCCACCAAGGTTCCAGCCTCCAAAGTGAAAGACGCCGTGGAACAGCAAGGGGAGGTGAAGAAGAA TAAAAGAGAAAGAAAGGAAGAACGGCAGAAGAAAAGGAAAAGAGAAAAGAAAGAACTAAAGTTAGAAAAC CACCAGGAAAACTCAAGGAATCAGAAGCCTAAGAAGCGCAAAAAGGGACAGGAGGCTGACCTTGAGGCTG GTGGGGAGGAAGTCCCTGAGGCCAATGGCTCTGCAGGGAAGAGGAGCAAGAAGAAGAAGCAGCGCAAGGA CAGCGCCAGTGAGGAAGAGGCACGCGTGGGCGCAGGGAAGAGGAAGCGGAGGCACTCGGAAGTTGAAACA GATTCTAAGAAGAAAAAGATGAAGCTCCCAGAGCATCCTGAGGGCGGAGAACCAGAAGACGATGAGGCTC CTGCAAAAGGTAAATTCAACTGGAAGGGAACTATTAAAGCAATTCTGAAACAGGCCCCAGACAATGAAAT AACCATCAAAAAGCTAAGGAAAAAGGTTTTAGCTCAGTACTACACAGTGACAGATGAGCATCACAGATCC GAAGAGGAACTCCTGGTCATCTTTAACAAGAAAATCAGCAAGAACCCTACCTTTAAGTTATTAAAGGACA AAGTCAAGCTTGTGAAA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >RC226844 protein sequence
Red=Cloning site Green=Tags(s) MVFFTCNACGESVKKIQVEKHVSVCRNCECLSCIDCGKDFWGDDYKNHVKCISEDQKYGGKGYEGKTHKG DIKQQAWIQKISELIKRPNVSPKVRELLEQISAFDNVPRKKAKFQNWMKNSLKVHNESILDQVWNIFSEA SNSEPVNKEQDQRPLHPVANPHAEISTKVPASKVKDAVEQQGEVKKNKRERKEERQKKRKREKKELKLEN HQENSRNQKPKKRKKGQEADLEAGGEEVPEANGSAGKRSKKKKQRKDSASEEEARVGAGKRKRRHSEVET DSKKKKMKLPEHPEGGEPEDDEAPAKGKFNWKGTIKAILKQAPDNEITIKKLRKKVLAQYYTVTDEHHRS EEELLVIFNKKISKNPTFKLLKDKVKLVK SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001145725 |
| ORF Size | 1137 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001145725.1, NP_001139197.1 |
| RefSeq Size | 1486 bp |
| RefSeq ORF | 1140 bp |
| Locus ID | 55646 |
| UniProt ID | Q9NX58 |
| MW | 43.6 kDa |
| Gene Summary | Plays a role in the maintenance of the appropriate processing of 47S/45S pre-rRNA to 32S/30S pre-rRNAs and their subsequent processing to produce 18S and 28S rRNAs (PubMed:24495227). Also acts at the level of transcription regulation. Along with PRMT5, binds the gamma-globin (HBG1/HBG2) promoter and represses its expression (PubMed:25092918). In neuroblastoma cells, may also repress the expression of oxidative stress genes, including CHAC1, HMOX1, SLC7A11, ULBP1 and SNORD41 that encodes a small nucleolar RNA (PubMed:28686580). Preferentially binds to a DNA motif containing 5'-GGTTAT-3' (PubMed:25092918). Stimulates phagocytosis of photoreceptor outer segments by retinal pigment epithelial cells (By similarity). Prevents nucleolin/NCL self-cleavage, maintaining a normal steady-state level of NCL protein in undifferentiated embryonic stem cells (ESCs), which in turn is essential for ESC self-renewal (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC226844L3 | Lenti ORF clone of Human Ly1 antibody reactive homolog (mouse) (LYAR), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC226844L4 | Lenti ORF clone of Human Ly1 antibody reactive homolog (mouse) (LYAR), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG226844 | LYAR (tGFP-tagged) - Human Ly1 antibody reactive homolog (mouse) (LYAR), transcript variant 2 |
CNY 4,370.00 |
|
| SC326467 | LYAR (untagged)-Human Ly1 antibody reactive homolog (mouse) (LYAR), transcript variant 2, mRNA |
CNY 7,220.00 |
