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CrkRS (CDK12) (NM_015083) Human Tagged ORF Clone
CAT＃: RC226399
CDK12 (Myc-DDK-tagged)-Human cyclin-dependent kinase 12 (CDK12), transcript variant 2
ORF Plasmid: tGFP
"NM_015083" in other vectors (6)
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CNY 10,136.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CRK7; CRKR; CRKRS |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC226399 representing NM_015083
Red=Cloning site Blue=ORF Green=Tags(s) CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGC GCCC ATGCCCAATTCAGAGAGACATGGGGGCAAGAAGGACGGGAGTGGAGGAGCTTCTGGAACTTTGCAGCCGT CATCGGGAGGCGGCAGCTCTAACAGCAGAGAGCGTCACCGCTTGGTATCGAAGCACAAGCGGCATAAGTC CAAACACTCCAAAGACATGGGGTTGGTGACCCCCGAAGCAGCATCCCTGGGCACAGTTATCAAACCTTTG GTGGAGTATGATGATATCAGCTCTGATTCCGACACCTTCTCCGATGACATGGCCTTCAAACTAGACCGAA GGGAGAACGACGAACGTCGTGGATCAGATCGGAGCGACCGCCTGCACAAACATCGTCACCACCAGCACAG GCGTTCCCGGGACTTACTAAAAGCTAAACAGACCGAAAAAGAAAAAAGCCAAGAAGTCTCCAGCAAGTCG GGATCGATGAAGGACCGGATATCGGGAAGTTCAAAGCGTTCGAATGAGGAGACTGATGACTATGGGAAGG CGCAGGTAGCCAAAAGCAGCAGCAAGGAATCCAGGTCATCCAAGCTCCACAAGGAGAAGACCAGGAAAGA ACGGGAGCTGAAGTCTGGGCACAAAGACCGGAGTAAAAGTCATCGAAAAAGGGAAACACCCAAAAGTTAC AAAACAGTGGACAGCCCAAAACGGAGATCCAGGAGCCCCCACAGGAAGTGGTCTGACAGCTCCAAACAAG ATGATAGCCCCTCGGGAGCTTCTTATGGCCAAGATTATGACCTTAGTCCCTCACGATCTCATACCTCGAG CAATTATGACTCCTACAAGAAAAGTCCTGGAAGTACCTCGAGAAGGCAGTCGGTCAGTCCCCCTTACAAG GAGCCTTCGGCCTACCAGTCCAGCACCCGGTCACCGAGCCCCTACAGTAGGCGACAGAGATCTGTCAGTC CCTATAGCAGGAGACGGTCGTCCAGCTACGAAAGAAGTGGCTCTTACAGCGGGCGATCGCCCAGTCCCTA TGGTCGAAGGCGGTCCAGCAGCCCTTTCCTGAGCAAGCGGTCTCTGAGTCGGAGTCCACTCCCCAGTAGG AAATCCATGAAGTCCAGAAGTAGAAGTCCTGCATATTCAAGACATTCATCTTCTCATAGTAAAAAGAAGA GATCCAGTTCACGCAGTCGTCATTCCAGTATCTCACCTGTCAGGCTTCCACTTAATTCCAGTCTGGGAGC TGAACTCAGTAGGAAAAAGAAGGAAAGAGCAGCTGCTGCTGCTGCAGCAAAGATGGATGGAAAGGAGTCC AAGGGTTCACCTGTATTTTTGCCTAGAAAAGAGAACAGTTCAGTAGAGGCTAAGGATTCAGGTTTGGAGT CTAAAAAGTTACCCAGAAGTGTAAAATTGGAAAAATCTGCCCCAGATACTGAACTGGTGAATGTAACACA TCTAAACACAGAGGTAAAAAATTCTTCAGATACAGGGAAAGTAAAGTTGGATGAGAACTCCGAGAAGCAT CTTGTTAAAGATTTGAAAGCACAGGGAACAAGAGACTCTAAACCCATAGCACTGAAAGAGGAGATTGTTA CTCCAAAGGAGACAGAAACATCAGAAAAGGAGACCCCTCCACCTCTTCCCACAATTGCTTCTCCCCCACC CCCTCTACCAACTACTACCCCTCCACCTCAGACACCCCCTTTGCCACCTTTGCCTCCAATACCAGCTCTT CCACAGCAACCACCTCTGCCTCCTTCTCAGCCAGCATTTAGTCAGGTTCCTGCTTCCAGTACTTCAACTT TGCCCCCTTCTACTCACTCAAAGACATCTGCTGTGTCCTCTCAGGCAAATTCTCAGCCCCCTGTACAGGT TTCTGTGAAGACTCAAGTATCTGTAACAGCTGCTATTCCACACCTGAAAACTTCAACGTTGCCTCCTTTG CCCCTCCCACCCTTATTACCTGGAGATGATGACATGGATAGTCCAAAAGAAACTCTTCCTTCAAAACCTG TGAAGAAAGAGAAGGAACAGAGGACACGTCACTTACTCACAGACCTTCCTCTCCCTCCAGAGCTCCCTGG TGGAGATCTGTCTCCCCCAGACTCTCCAGAACCAAAGGCAATCACACCACCTCAGCAACCATATAAAAAG AGACCAAAAATTTGTTGTCCTCGTTATGGAGAAAGAAGACAAACAGAAAGCGACTGGGGGAAACGCTGTG TGGACAAGTTTGACATTATTGGGATTATTGGAGAAGGAACCTATGGCCAAGTATATAAAGCCAAGGACAA AGACACAGGAGAACTAGTGGCTCTGAAGAAGGTGAGACTAGACAATGAGAAAGAGGGCTTCCCAATCACA GCCATTCGTGAAATCAAAATCCTTCGTCAGTTAATCCACCGAAGTGTTGTTAACATGAAGGAAATTGTCA CAGATAAACAAGATGCACTGGATTTCAAGAAGGACAAAGGTGCCTTTTACCTTGTATTTGAGTATATGGA CCATGACTTAATGGGACTGCTAGAATCTGGTTTGGTGCACTTTTCTGAGGACCATATCAAGTCGTTCATG AAACAGCTAATGGAAGGATTGGAATACTGTCACAAAAAGAATTTCCTGCATCGGGATATTAAGTGTTCTA ACATTTTGCTGAATAACAGTGGGCAAATCAAACTAGCAGATTTTGGACTTGCTCGGCTCTATAACTCTGA AGAGAGTCGCCCTTACACAAACAAAGTCATTACTTTGTGGTACCGACCTCCAGAACTACTGCTAGGAGAG GAACGTTACACACCAGCCATAGATGTTTGGAGCTGTGGATGTATTCTTGGGGAACTATTCACAAAGAAGC CTATTTTTCAAGCCAATCTGGAACTGGCTCAGCTAGAACTGATCAGCCGACTTTGTGGTAGCCCTTGTCC AGCTGTGTGGCCTGATGTTATCAAACTGCCCTACTTCAACACCATGAAACCGAAGAAGCAATATCGAAGG CGTCTACGAGAAGAATTCTCTTTCATTCCTTCTGCAGCACTTGATTTATTGGACCACATGCTGACACTAG ATCCTAGTAAGCGGTGCACAGCTGAACAGACCCTACAGAGCGACTTCCTTAAAGATGTCGAACTCAGCAA AATGGCTCCTCCAGACCTCCCCCACTGGCAGGATTGCCATGAGTTGTGGAGTAAGAAACGGCGACGTCAG CGACAAAGTGGTGTTGTAGTCGAAGAGCCACCTCCATCCAAAACTTCTCGAAAAGAAACTACCTCAGGGA CAAGTACTGAGCCTGTGAAGAACAGCAGCCCAGCACCACCTCAGCCTGCTCCTGGCAAGGTGGAGTCTGG GGCTGGGGATGCAATAGGCCTTGCTGACATCACACAACAGCTGAATCAAAGTGAATTGGCAGTGTTATTA AACCTGCTGCAGAGCCAAACCGACCTGAGCATCCCTCAAATGGCACAGCTGCTTAACATCCACTCCAACC CAGAGATGCAGCAGCAGCTGGAAGCCCTGAACCAATCCATCAGTGCCCTGACGGAAGCTACTTCCCAGCA GCAGGACTCAGAGACCATGGCCCCAGAGGAGTCTTTGAAGGAAGCACCCTCTGCCCCAGTGATCCTGCCT TCAGCAGAACAGATGACCCTTGAAGCTTCAAGCACACCAGCTGACATGCAGAATATATTGGCAGTTCTCT TGAGTCAGCTGATGAAAACCCAAGAGCCAGCAGGCAGTCTGGAGGAAAACAACAGTGACAAGAACAGTGG GCCACAGGGGCCCCGAAGAACTCCCACAATGCCACAGGAGGAGGCAGCAGAGAAGAGGCCCCCTGAGCCC CCCGGACCTCCACCGCCGCCACCTCCACCCCCTCTGGTTGAAGGCGATCTTTCCAGCGCCCCCCAGGAGT TGAACCCAGCCGTGACAGCCGCCTTGCTGCAACTTTTATCCCAGCCTGAAGCAGAGCCTCCTGGCCACCT GCCACATGAGCACCAGGCCTTGAGACCAATGGAGTACTCCACCCGACCCCGTCCAAACAGGACTTATGGA AACACTGATGGGCCTGAAACAGGGTTCAGTGCCATTGACACTGATGAACGAAACTCTGGTCCAGCCTTGA CAGAATCCTTGGTCCAGACCCTGGTGAAGAACAGGACCTTCTCAGGCTCTCTGAGCCACCTTGGGGAGTC CAGCAGTTACCAGGGCACAGGGTCAGTGCAGTTTCCAGGGGACCAGGACCTCCGTTTTGCCAGGGTCCCC TTAGCGTTACACCCGGTGGTCGGGCAACCATTCCTGAAGGCTGAGGGAAGCAGCAATTCTGTGGTACATG CAGAGACCAAATTGCAAAACTATGGGGAGCTGGGGCCAGGAACCACTGGGGCCAGCAGCTCAGGAGCAGG CCTTCACTGGGGGGGCCCAACTCAGTCTTCTGCTTATGGAAAACTCTATCGGGGGCCTACAAGAGTCCCA CCAAGAGGGGGAAGAGGGAGAGGAGTTCCTTAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC226399 representing NM_015083
Red=Cloning site Green=Tags(s) MPNSERHGGKKDGSGGASGTLQPSSGGGSSNSRERHRLVSKHKRHKSKHSKDMGLVTPEAASLGTVIKPL VEYDDISSDSDTFSDDMAFKLDRRENDERRGSDRSDRLHKHRHHQHRRSRDLLKAKQTEKEKSQEVSSKS GSMKDRISGSSKRSNEETDDYGKAQVAKSSSKESRSSKLHKEKTRKERELKSGHKDRSKSHRKRETPKSY KTVDSPKRRSRSPHRKWSDSSKQDDSPSGASYGQDYDLSPSRSHTSSNYDSYKKSPGSTSRRQSVSPPYK EPSAYQSSTRSPSPYSRRQRSVSPYSRRRSSSYERSGSYSGRSPSPYGRRRSSSPFLSKRSLSRSPLPSR KSMKSRSRSPAYSRHSSSHSKKKRSSSRSRHSSISPVRLPLNSSLGAELSRKKKERAAAAAAAKMDGKES KGSPVFLPRKENSSVEAKDSGLESKKLPRSVKLEKSAPDTELVNVTHLNTEVKNSSDTGKVKLDENSEKH LVKDLKAQGTRDSKPIALKEEIVTPKETETSEKETPPPLPTIASPPPPLPTTTPPPQTPPLPPLPPIPAL PQQPPLPPSQPAFSQVPASSTSTLPPSTHSKTSAVSSQANSQPPVQVSVKTQVSVTAAIPHLKTSTLPPL PLPPLLPGDDDMDSPKETLPSKPVKKEKEQRTRHLLTDLPLPPELPGGDLSPPDSPEPKAITPPQQPYKK RPKICCPRYGERRQTESDWGKRCVDKFDIIGIIGEGTYGQVYKAKDKDTGELVALKKVRLDNEKEGFPIT AIREIKILRQLIHRSVVNMKEIVTDKQDALDFKKDKGAFYLVFEYMDHDLMGLLESGLVHFSEDHIKSFM KQLMEGLEYCHKKNFLHRDIKCSNILLNNSGQIKLADFGLARLYNSEESRPYTNKVITLWYRPPELLLGE ERYTPAIDVWSCGCILGELFTKKPIFQANLELAQLELISRLCGSPCPAVWPDVIKLPYFNTMKPKKQYRR RLREEFSFIPSAALDLLDHMLTLDPSKRCTAEQTLQSDFLKDVELSKMAPPDLPHWQDCHELWSKKRRRQ RQSGVVVEEPPPSKTSRKETTSGTSTEPVKNSSPAPPQPAPGKVESGAGDAIGLADITQQLNQSELAVLL NLLQSQTDLSIPQMAQLLNIHSNPEMQQQLEALNQSISALTEATSQQQDSETMAPEESLKEAPSAPVILP SAEQMTLEASSTPADMQNILAVLLSQLMKTQEPAGSLEENNSDKNSGPQGPRRTPTMPQEEAAEKRPPEP PGPPPPPPPPPLVEGDLSSAPQELNPAVTAALLQLLSQPEAEPPGHLPHEHQALRPMEYSTRPRPNRTYG NTDGPETGFSAIDTDERNSGPALTESLVQTLVKNRTFSGSLSHLGESSSYQGTGSVQFPGDQDLRFARVP LALHPVVGQPFLKAEGSSNSVVHAETKLQNYGELGPGTTGASSSGAGLHWGGPTQSSAYGKLYRGPTRVP PRGGRGRGVPY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
AscI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_015083 |
| ORF Size | 4443 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_015083.3 |
| RefSeq ORF | 4446 bp |
| Locus ID | 51755 |
| UniProt ID | Q9NYV4 |
| Protein Families | Druggable Genome, Protein Kinase |
| MW | 163 kDa |
| Gene Summary | Cyclin-dependent kinase that phosphorylates the C-terminal domain (CTD) of the large subunit of RNA polymerase II (POLR2A), thereby acting as a key regulator of transcription elongation. Regulates the expression of genes involved in DNA repair and is required for the maintenance of genomic stability. Preferentially phosphorylates 'Ser-5' in CTD repeats that are already phosphorylated at 'Ser-7', but can also phosphorylate 'Ser-2'. Required for RNA splicing, possibly by phosphorylating SRSF1/SF2. Involved in regulation of MAP kinase activity, possibly leading to affect the response to estrogen inhibitors.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC226399L1 | Lenti ORF clone of Human cyclin-dependent kinase 12 (CDK12), transcript variant 2, Myc-DDK-tagged |
CNY 12,536.00 |
|
| RC226399L2 | Lenti ORF clone of Human cyclin-dependent kinase 12 (CDK12), transcript variant 2, mGFP tagged |
CNY 16,720.00 |
|
| RC226399L3 | Lenti ORF clone of Human cyclin-dependent kinase 12 (CDK12), transcript variant 2, Myc-DDK-tagged |
CNY 12,536.00 |
|
| RC226399L4 | Lenti ORF clone of Human cyclin-dependent kinase 12 (CDK12), transcript variant 2, mGFP tagged |
CNY 12,536.00 |
|
| RG226399 | CDK12 (tGFP-tagged) - Human cyclin-dependent kinase 12 (CDK12), transcript variant 2 |
CNY 16,340.00 |
|
| SC317169 | CDK12 (untagged)-Human cyclin-dependent kinase 12 (CDK12), transcript variant 2 |
CNY 10,450.00 |
