UBE4B (NM_001105562) Human Tagged Lenti ORF Clone
CAT#: RC226378L3
- LentiORF®
Lenti-ORF clone of UBE4B (Myc-DDK-tagged)-Human ubiquitination factor E4B (UBE4B), transcript variant 1
"NM_001105562" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 14,920.00
CNY 600.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | E4; HDNB1; UBOX3; UFD2; UFD2A |
Vector | pLenti-C-Myc-DDK-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(RC226378).
|
Restriction Sites | SgfI-RsrII Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001105562 |
ORF Size | 3906 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001105562.1 |
RefSeq ORF | 3909 bp |
Locus ID | 10277 |
UniProt ID | O95155 |
Protein Pathways | Ubiquitin mediated proteolysis |
MW | 146 kDa |
Gene Summary | The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes an additional conjugation factor, E4, which is involved in multiubiquitin chain assembly. This gene is also the strongest candidate in the neuroblastoma tumor suppressor genes. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC226378 | UBE4B (Myc-DDK-tagged)-Human ubiquitination factor E4B (UBE4B), transcript variant 1 |
CNY 13,600.00 |
|
RC226378L4 | Lenti-ORF clone of UBE4B (mGFP-tagged)-Human ubiquitination factor E4B (UBE4B), transcript variant 1 |
CNY 14,920.00 |
|
RG226378 | UBE4B (tGFP-tagged) - Human ubiquitination factor E4B (UBE4B), transcript variant 1 |
CNY 14,350.00 |
|
SC317163 | UBE4B (untagged)-Human ubiquitination factor E4B (UBE4B), transcript variant 1 |
CNY 25,180.00 |