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ORP150 (HYOU1) (NM_001130991) Human Tagged ORF Clone
CAT＃: RC226294
- TrueORF®
HYOU1 (Myc-DDK-tagged)-Human hypoxia up-regulated 1 (HYOU1), transcript variant 2
ORF Plasmid: tGFP
"NM_001130991" in other vectors (4)
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CNY 7,130.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | GRP-170; Grp170; HSP12A; IMD59; ORP-150; ORP150 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC226294 representing NM_001130991
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGACAAAGTTAGGAGGCAGAGGCCGAGGAGGCGAGTCTGTTGGGCCTTGGTGGCTGTGCTCTTGG CAGACCTGTTGGCACTGAGTGATACACTGGCAGTGATGTCTGTGGACCTGGGCAGTGAGTCCATGAAGGT GGCCATTGTCAAACCTGGAGTGCCCATGGAAATTGTCTTGAATAAGGAATCTCGGAGGAAAACACCGGTG ATCGTGACCCTGAAAGAAAATGAAAGATTCTTTGGAGACAGTGCAGCAAGCATGGCGATTAAGAATCCAA AGGCTACGCTACGTTACTTCCAGCACCTCCTGGGGAAGCAGGCAGATAACCCCCATGTAGCTCTTTACCA GGCCCGCTTCCCGGAGCACGAGCTGACTTTCGACCCACAGAGGCAGACTGTGCACTTTCAGATCAGCTCG CAGCTGCAGTTCTCACCTGAGGAAGTGTTGGGCATGGTTCTCAATTATTCTCGTTCTCTAGCTGAAGATT TTGCAGAGCAGCCCATCAAGGATGCAGTGATCACCGTGCCAGTCTTCTTCAACCAGGCCGAGCGCCGAGC TGTGCTGCAGGCTGCTCGTATGGCTGGCCTCAAAGTGCTGCAGCTCATCAATGACAACACCGCCACTGCC CTCAGCTATGGTGTCTTCCGCCGGAAAGATATTAACACCACTGCCCAGAATATCATGTTCTATGACATGG GCTCAGGCAGCACCGTATGCACCATTGTGACCTACCAGATGGTGAAGACTAAGGAAGCTGGGATGCAGCC ACAGCTGCAGATCCGGGGAGTAGGATTTGACCGTACCCTGGGGGGCCTGGAGATGGAGCTCCGGCTTCGA GAACGCCTGGCTGGGCTTTTCAATGAGCAGCGCAAGGGTCAGAGAGCAAAGGATGTGCGGGAGAACCCGC GTGCCATGGCCAAGCTGCTGCGTGAGGCTAATCGGCTCAAAACCGTCCTCAGTGCCAACGCTGACCACAT GGCACAGATTGAAGGCCTGATGGATGATGTGGACTTCAAGGCAAAAGTGACTCGTGTGGAATTTGAGGAG TTGTGTGCAGACTTGTTTGAGCGGGTGCCTGGGCCTGTACAGCAGGCCCTCCAGAGTGCCGAAATGAGTC TGGATGAGATTGAGCAGGTGATCCTGGTGGGTGGGGCCACTCGGGTCCCCAGAGTTCAGGAGGTGCTGCT GAAGGCCGTGGGCAAGGAGGAGCTGGGGAAGAACATCAATGCAGATGAAGCAGCCGCCATGGGGGCAGTG TACCAGGCAGCTGCGCTCAGCAAAGCCTTTAAAGTGAAGCCATTTGTCGTCCGAGATGCAGTGGTCTACC CCATCCTGGTGGAGTTCACGAGGGAGGTGGAGGAGGAGCCTGGGATTCACAGCCTGAAGCACAATAAACG GGTACTCTTCTCTCGGATGGGGCCCTACCCTCAACGCAAAGTCATCACCTTTAACCGCTACAGCCATGAT TTCAACTTCCACATCAACTACGGCGACCTGGGCTTCCTGGGGCCTGAAGATCTTCGGGTATTTGGCTCCC AGAATCTGACCACAGTGAAGCTAAAAGGGGTGGGTGACAGCTTCAAGAAGTATCCTGACTACGAGTCCAA GGGCATCAAGGCTCACTTCAACCTGGATGAGAGTGGCGTGCTCAGTCTAGACAGGGTGGAGTCTGTATTT GAGACACTGGTAGAGGACAGCGCAGAAGAGGAATCTACTCTCACCAAACTTGGCAACACCATTTCCAGCC TGTTTGGAGGCGGTACCACACCAGATGCCAAGGAGAATGGTACTGATACTGTCCAGGAGGAAGAGGAGAG CCCTGCAGAGGGGAGCAAGGACGAGCCTGGGGAGCAGGTGGAGCTCAAGGAGGAAGCTGAGGCCCCAGTG GAGGATGGCTCTCAGCCCCCACCCCCTGAACCTAAGGGAGATGCAACCCCTGAGGGAGAAAAGGCCACAG AAAAAGAAAATGGGGACAAGTCTGAGGCCCAGAAACCAAGTGAGAAGGCAGAGGCAGGGCCTGAGGGCGT CGCTCCAGCCCCAGAGGGAGAGAAGAAGCAGAAGCCCGCCAGGAAGCGGCGAATGGTAGAGGAGATCGGG GTGGAGCTGGTTGTTCTGGACCTGCCTGACTTGCCAGAGGATAAGCTGGCTCAGTCGGTGCAGAAACTTC AGGACTTGACACTCCGAGACCTGGAGAAGCAGGAACGGGAAAAAGCTGCCAACAGCTTGGAAGCATTCAT ATTTGAGACCCAGGACAAGCTGTACCAGCCCGAGTACCAGGAAGTGTCCACAGAGGAGCAGCGTGAGGAG ATCTCTGGGAAGCTCAGCGCCGCATCCACCTGGCTGGAGGATGAGGGTGTTGGAGCCACCACAGTGATGT TGAAGGAGAAGCTGGCTGAGCTGAGGAAGCTGTGCCAAGGGCTGTTTTTTCGGGTAGAGGAGCGCAAGAA GTGGCCCGAACGGCTGTCTGCCCTCGATAATCTCCTCAACCATTCCAGCATGTTCCTCAAGGGGGCCCGG CTCATCCCAGAGATGGACCAGATCTTCACTGAGGTGGAGATGACAACGTTAGAGAAAGTCATCAATGAGA CCTGGGCCTGGAAGAATGCAACTCTGGCCGAGCAGGCTAAGCTGCCCGCCACAGAGAAGCCTGTGTTGCT CTCAAAAGACATTGAAGCTAAGATGATGGCCCTGGACCGAGAGGTGCAGTATCTGCTCAATAAGGCCAAG TTTACCAAGCCCCGGCCCCGGCCTAAGGACAAGAATGGGACCCGGGCAGAGCCACCCCTCAATGCCAGTG CCAGTGACCAGGGGGAGAAGGTCATCCCTCCAGCAGGCCAGACTGAAGATGCAGAGCCCATTTCAGAACC TGAGAAAGTAGAGACTGGATCCGAGCCAGGAGACACTGAGCCTTTGGAGTTAGGAGGTCCTGGAGCAGAA CCTGAACAGAAAGAACAATCGACAGGACAGAAGCGGCCTTTGAAGAACGACGAACTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC226294 representing NM_001130991
Red=Cloning site Green=Tags(s) MADKVRRQRPRRRVCWALVAVLLADLLALSDTLAVMSVDLGSESMKVAIVKPGVPMEIVLNKESRRKTPV IVTLKENERFFGDSAASMAIKNPKATLRYFQHLLGKQADNPHVALYQARFPEHELTFDPQRQTVHFQISS QLQFSPEEVLGMVLNYSRSLAEDFAEQPIKDAVITVPVFFNQAERRAVLQAARMAGLKVLQLINDNTATA LSYGVFRRKDINTTAQNIMFYDMGSGSTVCTIVTYQMVKTKEAGMQPQLQIRGVGFDRTLGGLEMELRLR ERLAGLFNEQRKGQRAKDVRENPRAMAKLLREANRLKTVLSANADHMAQIEGLMDDVDFKAKVTRVEFEE LCADLFERVPGPVQQALQSAEMSLDEIEQVILVGGATRVPRVQEVLLKAVGKEELGKNINADEAAAMGAV YQAAALSKAFKVKPFVVRDAVVYPILVEFTREVEEEPGIHSLKHNKRVLFSRMGPYPQRKVITFNRYSHD FNFHINYGDLGFLGPEDLRVFGSQNLTTVKLKGVGDSFKKYPDYESKGIKAHFNLDESGVLSLDRVESVF ETLVEDSAEEESTLTKLGNTISSLFGGGTTPDAKENGTDTVQEEEESPAEGSKDEPGEQVELKEEAEAPV EDGSQPPPPEPKGDATPEGEKATEKENGDKSEAQKPSEKAEAGPEGVAPAPEGEKKQKPARKRRMVEEIG VELVVLDLPDLPEDKLAQSVQKLQDLTLRDLEKQEREKAANSLEAFIFETQDKLYQPEYQEVSTEEQREE ISGKLSAASTWLEDEGVGATTVMLKEKLAELRKLCQGLFFRVEERKKWPERLSALDNLLNHSSMFLKGAR LIPEMDQIFTEVEMTTLEKVINETWAWKNATLAEQAKLPATEKPVLLSKDIEAKMMALDREVQYLLNKAK FTKPRPRPKDKNGTRAEPPLNASASDQGEKVIPPAGQTEDAEPISEPEKVETGSEPGDTEPLELGGPGAE PEQKEQSTGQKRPLKNDEL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001130991 |
| ORF Size | 2997 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001130991.3 |
| RefSeq ORF | 3000 bp |
| Locus ID | 10525 |
| UniProt ID | Q9Y4L1 |
| Protein Families | Transmembrane |
| MW | 111.34 kDa |
| Gene Summary | The protein encoded by this gene belongs to the heat shock protein 70 family. This gene uses alternative transcription start sites. A cis-acting segment found in the 5' UTR is involved in stress-dependent induction, resulting in the accumulation of this protein in the endoplasmic reticulum (ER) under hypoxic conditions. The protein encoded by this gene is thought to play an important role in protein folding and secretion in the ER. Since suppression of the protein is associated with accelerated apoptosis, it is also suggested to have an important cytoprotective role in hypoxia-induced cellular perturbation. This protein has been shown to be up-regulated in tumors, especially in breast tumors, and thus it is associated with tumor invasiveness. This gene also has an alternative translation initiation site, resulting in a protein that lacks the N-terminal signal peptide. This signal peptide-lacking protein, which is only 3 amino acids shorter than the mature protein in the ER, is thought to have a housekeeping function in the cytosol. In rat, this protein localizes to both the ER by a carboxy-terminal peptide sequence and to mitochondria by an amino-terminal targeting signal. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC226294L3 | Lenti-ORF clone of HYOU1 (Myc-DDK-tagged)-Human hypoxia up-regulated 1 (HYOU1), transcript variant 2 |
CNY 9,030.00 |
|
| RC226294L4 | Lenti-ORF clone of HYOU1 (mGFP-tagged)-Human hypoxia up-regulated 1 (HYOU1), transcript variant 2 |
CNY 9,030.00 |
|
| RG226294 | HYOU1 (tGFP-tagged) - Human hypoxia up-regulated 1 (HYOU1), transcript variant 2 |
CNY 7,890.00 |
|
| SC326310 | HYOU1 (untagged)-Human hypoxia up-regulated 1 (HYOU1), transcript variant 2 |
CNY 19,190.00 |
