NEK10 (NM_199347) Human Tagged ORF Clone

CAT＃: RC226105

  • TrueORF®

NEK10 (Myc-DDK-tagged)-Human NIMA (never in mitosis gene a)- related kinase 10 (NEK10)

ORF Plasmid: DDK tGFP

Datasheet
SDS
Plasmid Map

  "NM_199347" in other vectors (4)

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CNY 7,130.00

货期*
4周
规格
    • 10 ug

Product images

经常一起买 (5)
PS100001
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00

添加到购物车
CC100003
DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00

暂停销售
PRIMER
Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

添加到购物车
TA592569S
DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

添加到购物车

【特别福利】购一抗正装产品，免费领10ul抗体试用装

TA350923
Rabbit Polyclonal Anti-NEK10 Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

添加到购物车

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms CILD44
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC226105 representing NM_199347
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCTGATCAAGATAAAAAGGTGAAGACCACAGAAAAATCAACTGATAAACAGCAAGAAATCACCATCA
GGGACTATTCAGATCTTAAAAGACTTCGGTGCCTTTTGAACGTCCAATCAAGCAAACAACAGCTTCCAGC
CATTAACTTCGATAGTGCCCAAAATAGCATGACGAAGTCTGAGCCCGCCATCAGGGCGGGTGGACACAGA
GCTCGGGGTCAGTGGCATGAATCCACAGAAGCTGTTGAACTTGAAAATTTTAGTATAAACTACAAGAATG
AGAGAAATTTCAGCAAACATCCTCAGCGTAAACTATTTCAGGAGATCTTTACCGCCTTGGTGAAAAATAG
ACTCATAAGCAGAGAGTGGGTTAATCGAGCCCCATCTATTCATTTTCTGAGAGTGTTAATCTGTCTGAGG
CTACTAATGAGGGATCCATGTTATCAGGAAATACTCCATAGCTTGGGTGGGATTGAAAACCTAGCTCAGT
ATATGGAGATTGTAGCCAATGAGTACCTCGGCTATGGAGAAGAGCAGCACACTGTGGACAAGCTGGTCAA
CATGACATATATTTTTCAAAAACTTGCTGCAGTCAAAGATCAAAGAGAATGGGTCACCACAAGTGGAGCC
CACAAGACATTAGTAAATTTACTTGGTGCCCGAGATACTAATGTTCTATTGGGTTCCCTTCTGGCTCTGG
CTAGTTTAGCAGAAAGTCAAGAATGTAGGGAGAAGATAAGTGAACTCAACATTGTAGAAAATCTGTTGAT
GATTTTACATGAATATGACTTGCTTTCTAAAAGACTAACAGCGGAGTTGCTGCGCCTACTTTGTGCAGAG
CCCCAGGTGAAAGAGCAGGTGAAGCTCTATGAGGGGATACCGGTCCTCCTCAGTCTGCTCCACTCTGACC
ACTTGAAGCTCCTCTGGAGCATTGTCTGGATTCTGGTACAGGTTTGTGAGGACCCTGAGACCAGCGTGGA
AATTCGCATTTGGGGAGGCATCAAACAGCTTCTTCATATTTTACAAGGAGACAGAAATTTTGTTTCTGAT
CACTCCTCCATTGGAAGCCTGTCCAGTGCAAATGCTGCAGGCCGAATCCAGCAGCTTCATTTATCAGAAG
ACTTGAGCCCTAGGGAAATACAAGAAAATACTTTCTCACTTCAAGCAGCCTGCTGTGCTGCCCTCACTGA
GCTGGTGCTCAATGACACCAATGCCCACCAGGTGGTTCAGGAAAATGGTGTATATACAATAGCAAAATTA
ATTTTACCAAATAAGCAAAAGAATGCAGCAAAAAGTAATCTATTACAGTGTTATGCTTTCAGAGCCTTGA
GATTTCTCTTCAGTATGGAAAGAAACAGACCACTCTTTAAAAGACTTTTCCCCACAGACTTGTTTGAGAT
CTTCATTGACATAGGGCATTATGTACGTGATATCAGTGCTTATGAAGAATTGGTATCCAAGCTGAATTTA
TTAGTGGAGGATGAACTGAAGCAAATTGCTGAAAATATTGAAAGCATTAATCAGAACAAAGCTCCTTTGA
AATATATAGGCAACTATGCAATTTTGGATCATCTTGGAAGTGGAGCTTTTGGCTGTGTTTACAAGGTTAG
AAAGCATAGTGGTCAAAATCTTTTAGCAATGAAAGAGGTCAATTTACATAACCCAGCATTTGGAAAGGAT
AAGAAAGATCGAGACAGCAGCGTAAGGAATATTGTTTCTGAATTAACAATAATTAAAGAGCAGCTTTATC
ATCCCAACATTGTACGTTATTACAAAACATTTCTGGAAAATGATAGGTTGTACATAGTTATGGAGCTGAT
AGAAGGAGCCCCGCTTGGAGAGCATTTCAGTTCTTTGAAGGAAAAACATCACCATTTTACTGAAGAAAGA
CTATGGAAAATATTTATACAGCTGTGCTTAGCTCTTCGATACTTACACAAGGAGAAGAGGATTGTCCATA
GAGATCTGACACCAAACAACATTATGTTGGGGGATAAGGACAAAGTAACCGTTACTGACTTTGGCCTGGC
AAAGCAAAAACAAGAAAACAGTAAACTCACCTCTGTGGTTGGAACAATCCTGTATTCTTGTGTGCAGCAC
CTCTACCTTCGCTCTCCTGCTCCTGCTCTGGCCACA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
>RC226105 representing NM_199347
Red=Cloning site Green=Tags(s)

MPDQDKKVKTTEKSTDKQQEITIRDYSDLKRLRCLLNVQSSKQQLPAINFDSAQNSMTKSEPAIRAGGHR
ARGQWHESTEAVELENFSINYKNERNFSKHPQRKLFQEIFTALVKNRLISREWVNRAPSIHFLRVLICLR
LLMRDPCYQEILHSLGGIENLAQYMEIVANEYLGYGEEQHTVDKLVNMTYIFQKLAAVKDQREWVTTSGA
HKTLVNLLGARDTNVLLGSLLALASLAESQECREKISELNIVENLLMILHEYDLLSKRLTAELLRLLCAE
PQVKEQVKLYEGIPVLLSLLHSDHLKLLWSIVWILVQVCEDPETSVEIRIWGGIKQLLHILQGDRNFVSD
HSSIGSLSSANAAGRIQQLHLSEDLSPREIQENTFSLQAACCAALTELVLNDTNAHQVVQENGVYTIAKL
ILPNKQKNAAKSNLLQCYAFRALRFLFSMERNRPLFKRLFPTDLFEIFIDIGHYVRDISAYEELVSKLNL
LVEDELKQIAENIESINQNKAPLKYIGNYAILDHLGSGAFGCVYKVRKHSGQNLLAMKEVNLHNPAFGKD
KKDRDSSVRNIVSELTIIKEQLYHPNIVRYYKTFLENDRLYIVMELIEGAPLGEHFSSLKEKHHHFTEER
LWKIFIQLCLALRYLHKEKRIVHRDLTPNNIMLGDKDKVTVTDFGLAKQKQENSKLTSVVGTILYSCVQH
LYLRSPAPALAT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_199347
ORF Size 2136 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_199347.4
RefSeq ORF 2139 bp
Locus ID 152110
UniProt ID Q6ZWH5
Protein Families Druggable Genome, Protein Kinase
MW 81.4 kDa
Gene Summary Plays a role in the cellular response to UV irradiation. Mediates G2/M cell cycle arrest, MEK autoactivation and ERK1/2-signaling pathway activation in response to UV irradiation.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

SKU Description Size Price
RC226105L3 Lenti-ORF clone of NEK10 (Myc-DDK-tagged)-Human NIMA (never in mitosis gene a)- related kinase 10 (NEK10)
    • 10 ug

CNY 9,030.00

RC226105L4 Lenti-ORF clone of NEK10 (mGFP-tagged)-Human NIMA (never in mitosis gene a)- related kinase 10 (NEK10)
    • 10 ug

CNY 9,030.00

RG226105 NEK10 (tGFP-tagged) - Human NIMA (never in mitosis gene a)- related kinase 10 (NEK10)
    • 10 ug

CNY 7,890.00

SC326195 NEK10 (untagged)-Human NIMA (never in mitosis gene a)- related kinase 10 (NEK10)
    • 10 ug

CNY 13,680.00

You may also need
plasmid icon Empty Vector
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RC226105L4V

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RC226105L3V

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RC226105L3

Lenti-ORF clone of NEK10 (Myc-DDK-tagged)-Human NIMA (never in mitosis gene a)- related kinase 10 (NEK10)

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RC226105L4

Lenti-ORF clone of NEK10 (mGFP-tagged)-Human NIMA (never in mitosis gene a)- related kinase 10 (NEK10)

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RG226105

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