ZNF195 (NM_001130519) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	HRF1; ZNFP104
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC226013 representing NM_001130519 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTCTGTTGACGTTCAGGGATGTGGCCATAGAATTCTCCCTGGAGGAGTGGAAATGCCTGGACCTCG CTCAGCAGAATTTGTACAGGGATGTGATGTTGGAGAACTACAGAAACTTGTTCTCCGTTGGTCTCACTGT CTGTAAGCCAGGCCTGATCACCTGCCTGGAGCAACGAAAAGAGCCCTGGAATGTGAAGAGACAGGAGGCA GCAGACGGACATCCAGAGATGGGATTTCACCATGCTACTCAGGCTTGTCTTGAACTCCTGGGCTCAAGCG ATCTGCCTGCCTCAGCCTCCCAAAGTGCTGGGATTACAGGTGTGAACCACCGTGCCCAGCCAGGCCTCAA TGTTTCTGTGGACAAATTCACTGCTATGTCTTCTCATTTTACCCAAGACCTTCTGCCAGAGCAGGGCATA CAAGATGCATTCCCAAAAAGAATACTGAGAGGATATGGAAATTGTGGCCTTGATAATTTATATTTAAGGA AAGACTGGGAAAGTTTAGATGAGTGTAAGTTGCAAAAAGATTATAATGGACTTAACCAATGTTCATCAAC TACCCATAGCAAAATCTTTCAATATAATAAATATGTTAAAATCTTTGATAACTTTTCAAATTTACATAGA CGTAATATAAGTAATACTGGAGAGAAACCTTTCAAATGTCAAGAATGTGGCAAATCCTTTCAAATGCTCT CATTCCTAACTGAACATCAGAAAATTCACACTGGAAAAAAATTCCAAAAATGTGGAGAATGTGGCAAAAC CTTTATCCAGTGCTCACACTTTACTGAACCTGAGAACATTGACACTGGAGAGAAACCTTACAAGTGTCAA GAATGTAACAACGTCATTAAAACTTGCTCAGTCCTTACTAAAAATAGAATTTATGCCGGAGGGGAACATT ACAGATGTGAAGAATTTGGCAAAGTATTTAACCAGTGCTCCCACCTTACTGAACATGAGCATGGTACTGA GGAAAAACCCTGCAAATATGAAGAGTGCAGCAGTGTCTTTATCTCTTGCTCAAGCCTTTCTAATCAACAG ATGATTCTTGCTGGAGAGAAGCTCTCCAAATGTGAAACATGGTACAAAGGTTTTAACCACAGCCCAAATC CTTCCAAACACCAGAGAAATGAGATTGGAGGGAAACCTTTCAAATGTGAGGAATGTGACAGCATCTTCAA GTGGTTCTCAGACCTTACTAAACATAAGAGAATTCACACTGGTGAGAAACCATACAAATGTGACGAATGT GGGAAAGCCTATACACAGTCCTCACACCTCAGTGAACACAGGAGGATTCACACCGGAGAGAAACCCTACC AATGTGAAGAATGTGGGAAGGTCTTCAGAACTTGCTCAAGCCTTTCTAACCATAAGAGAACTCATTCTGA AGAAAAACCCTACACGTGTGAAGAATGTGGCAACATCTTTAAGCAGTTATCAGACCTCACTAAGCATAAG AAAACCCATACTGGAGAGAAGCCCTACAAATGTGACGAATGTGGAAAAAACTTTACCCAGTCCTCCAACC TTATTGTACATAAGAGAATTCATACTGGAGAGAAACCCTACAAGTGTGAAGAATGTGGCAGAGTCTTCAT GTGGTTCTCAGACATTACCAAACATAAGAAAACCCATACTGGAGAGAAACCCTACAAATGTGACGAATGT GGAAAAAACTTTACCCAGTCCTCAAACCTTATTGTACATAAGAGAATTCATACTGGAGAGAAACCCTACA AGTGTGAAAAGTGTGGCAAAGCCTTCACCCAGTTCTCACACCTGACTGTACATGAAAGCATTCATACT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC226013 representing NM_001130519 Red=Cloning site Green=Tags(s) MTLLTFRDVAIEFSLEEWKCLDLAQQNLYRDVMLENYRNLFSVGLTVCKPGLITCLEQRKEPWNVKRQEA ADGHPEMGFHHATQACLELLGSSDLPASASQSAGITGVNHRAQPGLNVSVDKFTAMSSHFTQDLLPEQGI QDAFPKRILRGYGNCGLDNLYLRKDWESLDECKLQKDYNGLNQCSSTTHSKIFQYNKYVKIFDNFSNLHR RNISNTGEKPFKCQECGKSFQMLSFLTEHQKIHTGKKFQKCGECGKTFIQCSHFTEPENIDTGEKPYKCQ ECNNVIKTCSVLTKNRIYAGGEHYRCEEFGKVFNQCSHLTEHEHGTEEKPCKYEECSSVFISCSSLSNQQ MILAGEKLSKCETWYKGFNHSPNPSKHQRNEIGGKPFKCEECDSIFKWFSDLTKHKRIHTGEKPYKCDEC GKAYTQSSHLSEHRRIHTGEKPYQCEECGKVFRTCSSLSNHKRTHSEEKPYTCEECGNIFKQLSDLTKHK KTHTGEKPYKCDECGKNFTQSSNLIVHKRIHTGEKPYKCEECGRVFMWFSDITKHKKTHTGEKPYKCDEC GKNFTQSSNLIVHKRIHTGEKPYKCEKCGKAFTQFSHLTVHESIHT TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001130519
ORF Size	1818 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001130519.3
RefSeq Size	3213 bp
RefSeq ORF	1821 bp
Locus ID	7748
UniProt ID	O14628
Protein Families	Transcription Factors
MW	69.7 kDa
Gene Summary	This gene encodes a protein belonging to the Krueppel C2H2-type zinc-finger protein family. These family members are transcription factors that are implicated in a variety of cellular processes. This gene is located near the centromeric border of chromosome 11p15.5, next to an imprinted domain that is associated with maternal-specific loss of heterozygosity in Wilms' tumors. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2012]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.