CAP1 (NM_001105530) Human Tagged ORF Clone

CAT#: RC225806

CAP1 (Myc-DDK-tagged)-Human CAP, adenylate cyclase-associated protein 1 (yeast) (CAP1), transcript variant 2

ORF Plasmid: DDK tGFP



  "NM_001105530" in other vectors (6)

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CNY 3,656.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


CAP1 Rabbit polyclonal Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms CAP; CAP1-PEN
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC225806 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGACATGCAAAATCTGGTAGAAAGATTGGAGAGGGCAGTGGGCCGCCTGGAGGCAGTATCTCATA
CCTCTGACATGCACCGTGGGTATGCAGACAGTCCTTCAAAAGCAGGAGCAGCTCCATATGTGCAGGCATT
TGACTCGCTGCTTGCTGGTCCTGTGGCAGAGTACTTGAAGATCAGTAAAGAGATTGGGGGAGACGTGCAG
AAACATGCGGAGATGGTCCACACAGGTTTGAAGTTGGAGCGAGCTCTGTTGGTTACAGCTTCTCAGTGTC
AACAGCCAGCAGAAAATAAGCTTTCCGATTTGTTGGCACCCATCTCAGAGCAGATCAAAGAAGTGATAAC
CTTTCGGGAGAAGAACCGAGGCAGCAAGTTGTTTAATCACCTGTCAGCTGTCAGCGAAAGTATCCAGGCC
CTGGGCTGGGTGGCTATGGCTCCCAAGCCTGGCCCTTATGTGAAAGAAATGAATGATGCCGCCATGTTTT
ATACAAACCGAGTCCTCAAAGAGTACAAAGATGTGGATAAGAAGCATGTAGACTGGGTCAAAGCTTATTT
AAGTATATGGACAGAGCTGCAGGCTTACATTAAGGAGTTCCATACCACCGGACTGGCCTGGAGCAAAACG
GGGCCTGTGGCAAAAGAACTGAGCGGACTGCCATCTGGACCCTCTGCCGGATCAGGTCCTCCTCCCCCTC
CACCAGGCCCCCCTCCTCCCCCAGTCTCTACCAGTTCAGGCTCAGATGAGTCTGCTTCCCGCTCAGCACT
GTTCGCGCAGATTAATCAGGGGGAGAGCATTACACATGCCCTGAAACATGTATCTGATGACATGAAGACT
CACAAGAACCCTGCCCTGAAGGCTCAGAGTGGTCCAGTACGCAGTGGCCCCAAACCATTCTCTGCACCTA
AACCCCAAACCAGCCCATCCCCCAAACGAGCCACAAAGAAGGAGCCAGCTGTACTTGAACTGGAGGGCAA
GAAGTGGAGAGTGGAAAATCAGGAAAATGTTTCCAACCTGGTGATTGAGGACACAGAGCTGAAACAGGTG
GCTTACATATACAAGTGTGTCAACACGACATTGCAAATCAAGGGCAAAATTAACTCCATTACAGTAGATA
ACTGTAAGAAACTTGGCCTGGTATTCGATGACGTGGTGGGCATTGTGGAGATAATCAACAGTAAGGATGT
CAAAGTTCAGGTAATGGGTAAAGTGCCAACCATATCCATCAACAAAACAGATGGCTGCCATGCTTACCTG
AGCAAGAATTCCCTGGATTGTGAAATAGTCAGTGCCAAATCTTCCGAGATGAATGTCCTCATTCCTACAG
AAGGCGGTGACTTTAATGAATTCCCAGTTCCTGAGCAGTTCAAGACCCTATGGAACGGGCAGAAGTTGGT
CACCACAGTGACAGAAATTGCTGGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC225806 protein sequence
Red=Cloning site Green=Tags(s)

MADMQNLVERLERAVGRLEAVSHTSDMHRGYADSPSKAGAAPYVQAFDSLLAGPVAEYLKISKEIGGDVQ
KHAEMVHTGLKLERALLVTASQCQQPAENKLSDLLAPISEQIKEVITFREKNRGSKLFNHLSAVSESIQA
LGWVAMAPKPGPYVKEMNDAAMFYTNRVLKEYKDVDKKHVDWVKAYLSIWTELQAYIKEFHTTGLAWSKT
GPVAKELSGLPSGPSAGSGPPPPPPGPPPPPVSTSSGSDESASRSALFAQINQGESITHALKHVSDDMKT
HKNPALKAQSGPVRSGPKPFSAPKPQTSPSPKRATKKEPAVLELEGKKWRVENQENVSNLVIEDTELKQV
AYIYKCVNTTLQIKGKINSITVDNCKKLGLVFDDVVGIVEIINSKDVKVQVMGKVPTISINKTDGCHAYL
SKNSLDCEIVSAKSSEMNVLIPTEGGDFNEFPVPEQFKTLWNGQKLVTTVTEIAG

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001105530
ORF Size 1425 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001105530.1, NP_001099000.1
RefSeq Size 2780 bp
RefSeq ORF 1428 bp
Locus ID 10487
UniProt ID Q01518
Protein Families Druggable Genome
MW 51.7 kDa
Gene Summary The protein encoded by this gene is related to the S. cerevisiae CAP protein, which is involved in the cyclic AMP pathway. The human protein is able to interact with other molecules of the same protein, as well as with CAP2 and actin. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2016]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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