SMAD9 (NM_001127217) Human Tagged ORF Clone
CAT#: RC225792
- TrueORF®
SMAD9 (Myc-DDK-tagged)-Human SMAD family member 9 (SMAD9), transcript variant a
ORF Plasmid: tGFP
"NM_001127217" in other vectors (4)
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CNY 3,656.00
CNY 3,990.00
Cited in 1 publication. |
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | MADH6; MADH9; PPH2; SMAD8; SMAD8/9; SMAD8A; SMAD8B |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC225792 representing NM_001127217
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCACTCCACCACCCCCATCAGCTCCCTCTTCTCCTTCACCAGCCCCGCAGTGAAGAGACTGCTAGGCT GGAAGCAAGGAGATGAAGAGGAAAAGTGGGCAGAGAAGGCAGTGGACTCTCTAGTGAAGAAGTTAAAGAA GAAGAAGGGAGCCATGGACGAGCTGGAGAGGGCTCTCAGCTGCCCGGGGCAGCCCAGCAAATGCGTCACG ATTCCCCGCTCCCTGGACGGGCGGCTGCAGGTGTCCCACCGCAAGGGCCTGCCCCATGTGATTTACTGTC GCGTGTGGCGCTGGCCGGATCTGCAGTCCCACCACGAGCTGAAGCCGCTGGAGTGCTGTGAGTTCCCATT TGGCTCCAAGCAGAAAGAAGTGTGCATTAACCCTTACCACTACCGCCGGGTGGAGACTCCAGTACTGCCT CCTGTGCTCGTGCCAAGACACAGTGAATATAACCCCCAGCTCAGCCTCCTGGCCAAGTTCCGCAGCGCCT CCCTGCACAGTGAGCCACTCATGCCACACAACGCCACCTATCCTGACTCTTTCCAGCAGCCTCCGTGCTC TGCACTCCCTCCCTCACCCAGCCACGCGTTCTCCCAGTCCCCGTGCACGGCCAGCTACCCTCACTCCCCA GGAAGTCCTTCTGAGCCAGAGAGTCCCTATCAACACTCAGTTGACACACCACCCCTGCCTTATCATGCCA CAGAAGCCTCTGAGACCCAGAGTGGCCAACCTGTAGATGCCACAGCTGATAGACATGTAGTGCTATCGAT ACCAAATGGAGACTTTCGACCAGTTTGTTACGAGGAGCCCCAGCACTGGTGCTCGGTCGCCTACTATGAA CTGAACAACCGAGTTGGGGAGACATTCCAGGCTTCCTCCCGAAGTGTGCTCATAGATGGGTTCACCGACC CTTCAAATAACAGGAACAGATTCTGTCTTGGACTTCTTTCTAATGTAAACAGAAACTCAACGATAGAAAA TACCAGGAGACATATAGGAAAGGGTGTGCACTTGTACTACGTCGGGGGAGAGGTGTATGCCGAGTGCGTG AGTGACAGCAGCATCTTTGTGCAGAGCCGGAACTGCAACTATCAACACGGCTTCCACCCAGCTACCGTCT GCAAGATCCCCAGCGGCTGCAGCCTCAAGGTCTTCAACAACCAGCTCTTCGCTCAGCTCCTGGCCCAGTC AGTTCACCACGGCTTTGAAGTCGTGTATGAACTGACCAAGATGTGTACTATCCGGATGAGTTTTGTTAAG GGTTGGGGTGCTGAGTATCATCGCCAGGATGTCACCAGCACCCCCTGCTGGATTGAGATTCATCTTCATG GGCCACTGCAGTGGCTGGACAAAGTTCTGACTCAGATGGGCTCTCCACATAACCCCATTTCTTCAGTGTC T AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >RC225792 representing NM_001127217
Red=Cloning site Green=Tags(s) MHSTTPISSLFSFTSPAVKRLLGWKQGDEEEKWAEKAVDSLVKKLKKKKGAMDELERALSCPGQPSKCVT IPRSLDGRLQVSHRKGLPHVIYCRVWRWPDLQSHHELKPLECCEFPFGSKQKEVCINPYHYRRVETPVLP PVLVPRHSEYNPQLSLLAKFRSASLHSEPLMPHNATYPDSFQQPPCSALPPSPSHAFSQSPCTASYPHSP GSPSEPESPYQHSVDTPPLPYHATEASETQSGQPVDATADRHVVLSIPNGDFRPVCYEEPQHWCSVAYYE LNNRVGETFQASSRSVLIDGFTDPSNNRNRFCLGLLSNVNRNSTIENTRRHIGKGVHLYYVGGEVYAECV SDSSIFVQSRNCNYQHGFHPATVCKIPSGCSLKVFNNQLFAQLLAQSVHHGFEVVYELTKMCTIRMSFVK GWGAEYHRQDVTSTPCWIEIHLHGPLQWLDKVLTQMGSPHNPISSVS SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001127217 |
ORF Size | 1401 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001127217.3 |
RefSeq ORF | 1404 bp |
Locus ID | 4093 |
UniProt ID | O15198 |
Protein Families | ES Cell Differentiation/IPS, Transcription Factors |
Protein Pathways | TGF-beta signaling pathway |
MW | 52.3 kDa |
Gene Summary | The protein encoded by this gene is a member of the SMAD family, which transduces signals from TGF-beta family members. The encoded protein is activated by bone morphogenetic proteins and interacts with SMAD4. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jan 2010] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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Altered microRNA Processing in Heritable Pulmonary Arterial Hypertension: an Important Role for Smad-8
,Kylie M Drake, Deborah Zygmunt, Lori Mavrakis, Phyllis Harbor, Lingli Wang, Suzy A. Comhair, Serpil C. Erzurum, and Micheala A Aldred,
Am. J. Respir. Crit. Care Med., Sep 2011; 10.1164/rccm.201106-1130OC.
[SMAD9]
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Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC225792L3 | Lenti-ORF clone of SMAD9 (Myc-DDK-tagged)-Human SMAD family member 9 (SMAD9), transcript variant a |
CNY 6,056.00 |
|
RC225792L4 | Lenti-ORF clone of SMAD9 (mGFP-tagged)-Human SMAD family member 9 (SMAD9), transcript variant a |
CNY 5,890.00 |
|
RG225792 | SMAD9 (tGFP-tagged) - Human SMAD family member 9 (SMAD9), transcript variant a |
CNY 5,256.00 |
|
SC323084 | SMAD9 (untagged)-Human SMAD family member 9 (SMAD9), transcript variant a |
CNY 3,656.00 |