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DNAJB5 (NM_001135004) Human Tagged ORF Clone
CAT＃: RC225584
DNAJB5 (Myc-DDK-tagged)-Human DnaJ (Hsp40) homolog, subfamily B, member 5 (DNAJB5), transcript variant 2
ORF Plasmid: tGFP
"NM_001135004" in other vectors (4)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Hsc40 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC225584 representing NM_001135004
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCGGCGCGGAGGCGGAGCCGTGGGGGCGGGCTCCCGGTCCCGCTATCGGCGGCCGGCGGGCAGGCG ACTCCTGTCCCGGGTGGAGGCGGCGGAGCCGGAGCCGGGGGAGGGGGCAGCGGCTGTCTCACGGACCACG GCGGCGCCCGCAGCTCCTCACCGCAGCACCCCCACTGCAGGCCCGAGGAGCTTTCCGGAGCTTCCCACAC TCCTGGGGAGAAGACTTCTTAGCCAGCTTGATGTTTAAAATTCAGCTGGAGCCCTTAAAACTTCGAGCGT GGACGCTGAATGGGTTTGTAAAGTTTCGAAACAAGGAGACCAGTGCTGGTCCAGTGGCTGTGATGGGAAA AGATTATTACAAGATTCTTGGGATCCCATCGGGGGCCAACGAGGATGAGATCAAGAAAGCCTACCGGAAG ATGGCCTTGAAGTACCACCCAGACAAGAATAAAGAACCCAACGCTGAGGAGAAGTTTAAGGAGATTGCAG AGGCCTATGATGTGCTAAGTGACCCCAAGAAACGGGGCCTGTATGACCAGTATGGGGAGGAAGGCCTGAA GACCGGCGGTGGCACATCAGGTGGCTCCAGTGGCTCCTTTCACTACACCTTTCATGGGGACCCCCATGCC ACCTTTGCCTCCTTCTTTGGTGGCTCCAACCCCTTCGATATCTTCTTTGCCAGCAGCCGCTCCACTCGGC CCTTCAGTGGCTTTGACCCAGATGACATGGATGTGGATGAAGATGAGGACCCATTTGGCGCTTTCGGCCG TTTTGGCTTCAATGGGCTGAGTAGGGGTCCAAGGCGAGCCCCAGAACCACTGTACCCTCGGCGCAAGGTG CAGGACCCCCCAGTGGTGCACGAGCTGCGGGTGTCCCTGGAGGAGATCTACCATGGCTCCACCAAGCGCA TGAAGATCACAAGGCGTCGCCTCAACCCTGATGGGCGAACTGTGCGCACCGAGGACAAGATCCTGCACAT AGTCATCAAGCGTGGCTGGAAGGAAGGCACCAAGATCACCTTCCCCAAAGAAGGCGACGCCACACCTGAC AACATCCCTGCTGACATCGTCTTTGTGCTCAAAGACAAGCCCCATGCACACTTCCGCCGAGATGGCACCA ACGTGCTCTACAGTGCCCTGATCAGCCTCAAGGAGGCGCTGTGTGGCTGCACTGTGAACATTCCCACTAT CGACGGCCGAGTGATCCCTTTGCCCTGCAATGATGTCATCAAGCCAGGCACCGTGAAGAGACTCCGTGGG GAGGGCCTTCCCTTCCCCAAAGTGCCAACTCAGCGAGGAGACCTCATTGTTGAGTTCAAAGTTCGCTTCC CAGACAGATTAACACCACAGACAAGACAGATCCTTAAGCAGCACCTACCCTGTTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC225584 representing NM_001135004
Red=Cloning site Green=Tags(s) MGGAEAEPWGRAPGPAIGGRRAGDSCPGWRRRSRSRGRGQRLSHGPRRRPQLLTAAPPLQARGAFRSFPH SWGEDFLASLMFKIQLEPLKLRAWTLNGFVKFRNKETSAGPVAVMGKDYYKILGIPSGANEDEIKKAYRK MALKYHPDKNKEPNAEEKFKEIAEAYDVLSDPKKRGLYDQYGEEGLKTGGGTSGGSSGSFHYTFHGDPHA TFASFFGGSNPFDIFFASSRSTRPFSGFDPDDMDVDEDEDPFGAFGRFGFNGLSRGPRRAPEPLYPRRKV QDPPVVHELRVSLEEIYHGSTKRMKITRRRLNPDGRTVRTEDKILHIVIKRGWKEGTKITFPKEGDATPD NIPADIVFVLKDKPHAHFRRDGTNVLYSALISLKEALCGCTVNIPTIDGRVIPLPCNDVIKPGTVKRLRG EGLPFPKVPTQRGDLIVEFKVRFPDRLTPQTRQILKQHLPCS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001135004 |
| ORF Size | 1386 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001135004.2, NP_001128476.2 |
| RefSeq ORF | 1149 bp |
| Locus ID | 25822 |
| UniProt ID | O75953 |
| MW | 51.69 kDa |
| Gene Summary | This gene encodes a member of the DNAJ heat shock protein 40 family of co-chaperone proteins. The encoded protein contains an N-terminal DNAJ domain and a C-terminal substrate binding domain but lacks the cysteine-rich domain found in other DNAJ family members. In mice, a multi-protein complex containing this protein, thioredoxin 1, and histone deacetylase 4, serves as a master negative regulator of cardiac hypertrophy. [provided by RefSeq, Mar 2017] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC225584L3 | Lenti ORF clone of Human DnaJ (Hsp40) homolog, subfamily B, member 5 (DNAJB5), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC225584L4 | Lenti ORF clone of Human DnaJ (Hsp40) homolog, subfamily B, member 5 (DNAJB5), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG225584 | DNAJB5 (tGFP-tagged) - Human DnaJ (Hsp40) homolog, subfamily B, member 5 (DNAJB5), transcript variant 2 |
CNY 4,370.00 |
|
| SC325855 | DNAJB5 (untagged)-Human DnaJ (Hsp40) homolog, subfamily B, member 5 (DNAJB5), transcript variant 2 |
CNY 6,180.00 |
