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Claudin 5 (CLDN5) (NM_001130861) Human Tagged ORF Clone
CAT＃: RC225423
CLDN5 (Myc-DDK-tagged)-Human claudin 5 (CLDN5), transcript variant 1
ORF Plasmid: tGFP
"NM_001130861" in other vectors (6)
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CNY 3,600.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AWAL; BEC1; CPETRL1; TMDVCF; TMVCF |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC225423 representing NM_001130861
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCCGCGCACGGATTGGCTGCTTCGGGCCGGGGGGCCGGGCCCGGGGGACAGAATCCGCCCCCGAAC CTTCAAAGAGGGTACCCCCCGGCAGGAGCTGGCAGACCCAGGAGGTGCGACAGACCCGCGGGGCAAACGG ACTGGGGCCAAGAGCCGGGAGCGCGGGCGCAAAGGCACCAGGGCCCGCCCAGGGCGCCGCGCAGCACGGC CTTGGGGGTTCTGCGGGCCTTCGGGTGCGCGTCTCGCCTCTAGCCATGGGGTCCGCAGCGTTGGAGATCC TGGGCCTGGTGCTGTGCCTGGTGGGCTGGGGGGGTCTGATCCTGGCGTGCGGGCTGCCCATGTGGCAGGT GACCGCCTTCCTGGACCACAACATCGTGACGGCGCAGACCACCTGGAAGGGGCTGTGGATGTCGTGCGTG GTGCAGAGCACCGGGCACATGCAGTGCAAAGTGTACGACTCGGTGCTGGCTCTGAGCACCGAGGTGCAGG CGGCGCGGGCGCTCACCGTGAGCGCCGTGCTGCTGGCGTTCGTTGCGCTCTTCGTGACCCTGGCGGGCGC GCAGTGCACCACCTGCGTGGCCCCGGGCCCGGCCAAGGCGCGTGTGGCCCTCACGGGAGGCGTGCTCTAC CTGTTTTGCGGGCTGCTGGCGCTCGTGCCACTCTGCTGGTTCGCCAACATTGTCGTCCGCGAGTTTTACG ACCCGTCTGTGCCCGTGTCGCAGAAGTACGAGCTGGGCGCAGCGCTGTACATCGGCTGGGCGGCCACCGC GCTGCTCATGGTAGGCGGCTGCCTCTTGTGCTGCGGCGCCTGGGTCTGCACCGGCCGTCCCGACCTCAGC TTCCCCGTGAAGTACTCAGCGCCGCGGCGGCCCACGGCCACCGGCGACTACGACAAGAAGAACTACGTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC225423 representing NM_001130861
Red=Cloning site Green=Tags(s) MTRARIGCFGPGGRARGTESAPEPSKRVPPGRSWQTQEVRQTRGANGLGPRAGSAGAKAPGPAQGAAQHG LGGSAGLRVRVSPLAMGSAALEILGLVLCLVGWGGLILACGLPMWQVTAFLDHNIVTAQTTWKGLWMSCV VQSTGHMQCKVYDSVLALSTEVQAARALTVSAVLLAFVALFVTLAGAQCTTCVAPGPAKARVALTGGVLY LFCGLLALVPLCWFANIVVREFYDPSVPVSQKYELGAALYIGWAATALLMVGGCLLCCGAWVCTGRPDLS FPVKYSAPRRPTATGDYDKKNYV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001130861 |
| ORF Size | 909 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001130861.1, NP_001124333.1 |
| RefSeq ORF | 912 bp |
| Locus ID | 7122 |
| UniProt ID | O00501 |
| Protein Families | Transmembrane |
| Protein Pathways | Cell adhesion molecules (CAMs), Leukocyte transendothelial migration, Tight junction |
| MW | 31.4 kDa |
| Gene Summary | This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets. Mutations in this gene have been found in patients with velocardiofacial syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, May 2018] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC225423L1 | Lenti ORF clone of Human claudin 5 (CLDN5), transcript variant 1, Myc-DDK-tagged |
CNY 6,000.00 |
|
| RC225423L2 | Lenti ORF clone of Human claudin 5 (CLDN5), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC225423L3 | Lenti ORF clone of Human claudin 5 (CLDN5), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC225423L4 | Lenti ORF clone of Human claudin 5 (CLDN5), transcript variant 1, mGFP tagged |
CNY 6,000.00 |
|
| RG225423 | CLDN5 (tGFP-tagged) - Human claudin 5 (CLDN5), transcript variant 1 |
CNY 5,200.00 |
|
| SC325743 | CLDN5 (untagged)-Human claudin 5 (CLDN5), transcript variant 1 |
CNY 3,600.00 |
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