APOLD1 (NM_001130415) Human Tagged ORF Clone

CAT#: RC225381

APOLD1 (Myc-DDK-tagged)-Human apolipoprotein L domain containing 1 (APOLD1), transcript variant 1

ORF Plasmid: DDK tGFP



  "NM_001130415" in other vectors (4)

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CNY 2,400.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Rabbit Polyclonal Anti-APOLD1 Antibody
    • 100 ul

CNY 5,250.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms VERGE
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC225381 representing NM_001130415
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTCCGCGCGCCGTGTCACCGGCTGCGGGCCAGGGGTACTCGGAAGGCGCGGGCAGGAGCCTGGCGAG
GATGCACCTTCCCCTGCCTTGGAAAGGGAATGGAGAGGCCGGCGGCCCGGGAGCCGCATGGGCCCGACGC
GCTGCGGCGCTTCCAGGGACTGCTGCTGGACCGCCGAGGCCGGCTGCACGGCCAGGTGCTGCGCCTGCGC
GAGGTGGCCCGGCGCCTGGAGCGCCTGCGCAGGCGCTCCCTCGTAGCCAACGTGGCCGGCAGCTCGCTGA
GCGCAACGGGCGCCCTCGCCGCCATCGTGGGGCTCTCGCTCAGCCCGGTCACCCTGGGGACCTCGCTGCT
GGTGTCGGCCGTGGGGCTGGGGGTGGCCACAGCCGGAGGGGCCGTCACCATCACGTCCGATCTCTCGCTG
ATCTTCTGCAACTCCCGGGAGCTGCGGAGGGTGCAGGAGATCGCGGCCACCTGCCAGGACCAGATGCGAG
AGATCCTGAGCTGCCTCGAGTTTTTCTGCCGCTGGCAGGGCTGCGGGGACCGCCAGCTGCTGCAGTGCGG
GAGGAACGCCTCCATCGCCCTGTACAATTCTGTCTACTTCATCGTCTTCTTTGGCTCACGTGGCTTCCTC
ATCCCCAGGCGGGCGGAGGGGGACACCAAGGTTAGCCAGGCCGTGCTGAAGGCCAAGATTCAGAAACTGG
CCGAGAGCCTGGAGTCCTGCACCGGGGCTCTGGACGAACTCAGCGAGCAGCTGGAGTCTCGGGTTCAGCT
CTGCACCAAGTCCAGTCGTGGCCACGACCTCAAGATCTCTGCTGACCAGCGTGCAGGGCTGTTTTTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC225381 representing NM_001130415
Red=Cloning site Green=Tags(s)

MFRAPCHRLRARGTRKARAGAWRGCTFPCLGKGMERPAAREPHGPDALRRFQGLLLDRRGRLHGQVLRLR
EVARRLERLRRRSLVANVAGSSLSATGALAAIVGLSLSPVTLGTSLLVSAVGLGVATAGGAVTITSDLSL
IFCNSRELRRVQEIAATCQDQMREILSCLEFFCRWQGCGDRQLLQCGRNASIALYNSVYFIVFFGSRGFL
IPRRAEGDTKVSQAVLKAKIQKLAESLESCTGALDELSEQLESRVQLCTKSSRGHDLKISADQRAGLFF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001130415
ORF Size 837 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001130415.2
RefSeq ORF 840 bp
Locus ID 81575
UniProt ID Q96LR9
Protein Families Transmembrane
MW 30.4 kDa
Gene Summary APOLD1 is an endothelial cell early response protein that may play a role in regulation of endothelial cell signaling and vascular function (Regard et al., 2004 [PubMed 15102925]).[supplied by OMIM, Dec 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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