Frequenin (NCS1) (NM_001128826) Human Tagged ORF Clone
CAT#: RC225173
NCS1 (Myc-DDK-tagged)-Human neuronal calcium sensor 1 (NCS1), transcript variant 2
ORF Plasmid: tGFP
"NM_001128826" in other vectors (4)
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CNY 2,400.00
CNY 3,990.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | FLUP; FREQ |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC225173 representing NM_001128826
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAACGATTACCGAGAAGGAGGTCCAGCAGTGGTACAAAGGCTTCATCAAGGACTGCCCCAGTGGGC AGCTGGATGCGGCAGGCTTCCAGAAGATCTACAAGCAATTCTTCCCGTTCGGAGACCCCACCAAGTTTGC CACATTTGTTTTCAACGTCTTTGATGAAAACAAGGACGGGCGAATTGAGTTCTCCGAGTTCATCCAGGCG CTGTCGGTGACCTCACGGGGAACCCTGGATGAGAAGCTACGGTGGGCCTTCAAGCTCTACGACTTGGACA ATGATGGCTACATCACCAGGAATGAGATGCTGGACATTGTGGATGCCATTTACCAGATGGTGGGGAATAC CGTGGAGCTCCCAGAGGAGGAGAACACTCCTGAGAAGAGGGTGGACCGGATCTTTGCCATGATGGATAAG AATGCCGACGGGAAGCTGACCCTGCAGGAGTTCCAGGAGGGTTCCAAGGCAGACCCGTCCATTGTGCAGG CGCTGTCCCTCTACGACGGGCTGGTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC225173 representing NM_001128826
Red=Cloning site Green=Tags(s) MATITEKEVQQWYKGFIKDCPSGQLDAAGFQKIYKQFFPFGDPTKFATFVFNVFDENKDGRIEFSEFIQA LSVTSRGTLDEKLRWAFKLYDLDNDGYITRNEMLDIVDAIYQMVGNTVELPEEENTPEKRVDRIFAMMDK NADGKLTLQEFQEGSKADPSIVQALSLYDGLV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001128826 |
ORF Size | 516 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001128826.2 |
RefSeq ORF | 519 bp |
Locus ID | 23413 |
UniProt ID | P62166 |
Protein Families | Druggable Genome |
MW | 19.5 kDa |
Gene Summary | This gene is a member of the neuronal calcium sensor gene family, which encode calcium-binding proteins expressed predominantly in neurons. The protein encoded by this gene regulates G protein-coupled receptor phosphorylation in a calcium-dependent manner and can substitute for calmodulin. The protein is associated with secretory granules and modulates synaptic transmission and synaptic plasticity. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC225173L3 | Lenti ORF clone of Human neuronal calcium sensor 1 (NCS1), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC225173L4 | Lenti ORF clone of Human neuronal calcium sensor 1 (NCS1), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
RG225173 | NCS1 (tGFP-tagged) - Human neuronal calcium sensor 1 (NCS1), transcript variant 2 |
CNY 4,370.00 |
|
SC322865 | NCS1 (untagged)-Human neuronal calcium sensor 1 (NCS1), transcript variant 2 |
CNY 3,990.00 |
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