SIDT1 (NM_017699) Human Tagged ORF Clone
CAT#: RC224950
SIDT1 (Myc-DDK-tagged)-Human SID1 transmembrane family, member 1 (SIDT1)
ORF Plasmid: tGFP
"NM_017699" in other vectors (6)
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CNY 6,056.00
Cited in 2 publications. |
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | SID-1; SID1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC224950 representing NM_017699
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGCGGCTGCCTGCGGCTCGCGCTGCTCTGCGCGCTGCCCTGGCTCCTGCTGGCGGCGTCGCCCGGGC ACCCGGCGAAATCCCCCAGGCAGCCCCCGGCACCGCGCCGCGACCCCTTCGACGCTGCCAGGGGCGCCGA TTTCGATCATGTCTACAGCGGGGTGGTGAACCTCAGCACCGAGAACATCTACTCTTTCAACTACACCAGC CAGCCCGACCAGGTGACAGCCGTGAGGGTGTATGTGAACAGTTCCTCTGAGAATCTCAACTACCCGGTCC TTGTTGTGGTTCGCCAGCAGAAAGAGGTGCTGTCCTGGCAGGTTCCTCTGCTCTTCCAAGGACTATACCA GAGGAGCTACAACTATCAAGAAGTGAGCCGCACCTTATGTCCCTCAGAAGCAACCAATGAGACGGGACCC TTGCAGCAACTGATATTTGTAGATGTCGCATCCATGGCACCCCTGGGTGCTCAGTACAAACTGCTAGTTA CCAAGCTGAAGCACTTCCAGCTCCGGACAAATGTTGCCTTTCACTTTACTGCCAGCCCCTCTCAACCTCA GTATTTTCTATACAAGTTTCCCAAAGACGTGGACTCAGTTATCATTAAAGTGGTGTCTGAAATGGCTTAT CCATGTTCTGTTGTCTCAGTCCAGAATATCATGTGCCCGGTGTATGATCTCGACCACAATGTGGAATTTA ATGGTGTCTATCAGTCCATGACCAAGAAAGCTGCCATCACGCTACAGAAGAAGGATTTTCCAGGCGAGCA GTTCTTCGTGGTATTTGTGATAAAGCCTGAAGATTATGCCTGTGGAGGATCTTTCTTCATCCAGGAAAAG GAAAACCAGACCTGGAATCTACAGCGAAAAAAGAACCTTGAAGTGACCATTGTCCCTTCCATTAAAGAAT CTGTTTATGTGAAATCCAGTCTTTTCAGTGTCTTCATCTTCCTGTCCTTCTACTTGGGATGCCTTCTTGT TGGGTTTGTTCATTATCTGAGGTTTCAGAGAAAATCCATTGATGGAAGCTTTGGGTCCAATGATGGCTCT GGAAATATGGTGGCATCTCATCCCATTGCTGCCAGCACACCCGAAGGGAGCAATTATGGGACAATAGATG AGTCAAGCTCCAGTCCTGGAAGGCAGATGTCCTCCTCCGATGGTGGGCCACCGGGCCAGTCAGACACAGA CAGCTCCGTGGAGGAGAGCGACTTCGACACCATGCCAGACATTGAGAGTGATAAAAACATCATCCGGACC AAGATGTTCCTTTACCTGTCAGATTTGTCCAGGAAGGACCGGAGAATTGTCAGCAAAAAATATAAAATTT ATTTTTGGAACATCATCACCATTGCTGTGTTTTACGCGCTGCCCGTGATCCAGCTGGTCATTACCTATCA GACAGTGGTAAATGTCACTGGCAACCAGGACATCTGTTACTACAACTTCCTCTGTGCTCACCCCTTGGGC GTCCTGAGTGCCTTCAACAACATTCTCAGCAATCTGGGCCACGTGCTTCTGGGCTTCCTCTTCCTGCTGA TAGTCTTGCGCCGCGACATCCTCCATCGGAGAGCCCTGGAAGCCAAGGACATCTTTGCTGTGGAGTACGG GATTCCCAAACACTTTGGTCTCTTCTACGCTATGGGCATTGCATTGATGATGGAAGGGGTGCTCAGTGCT TGCTACCATGTCTGCCCTAATTATTCCAACTTCCAATTCGACACCTCCTTCATGTACATGATCGCTGGCC TGTGCATGCTGAAGCTCTATCAGACCCGCCACCCAGACATCAATGCCAGCGCCTACTCTGCCTATGCCTC CTTTGCTGTGGTCATCATGGTCACCGTCCTTGGAGTGGTGTTTGGAAAAAATGACGTATGGTTCTGGGTC ATCTTCTCTGCAATCCACGTTCTGGCCTCGCTAGCCCTCAGCACCCAGATATATTATATGGGTCGTTTCA AGATAGATTTGGGAATTTTCCGGCGGGCTGCCATGGTGTTCTACACAGACTGTATCCAGCAGTGTAGCCG ACCTCTATATATGGATAGAATGGTGTTGCTGGTTGTGGGGAATCTGGTTAACTGGTCCTTCGCCCTCTTT GGATTGATATACCGCCCCAGGGACTTTGCTTCCTACATGCTGGGCATCTTCATCTGTAACCTTTTGCTGT ACCTGGCCTTTTACATCATCATGAAGCTCCGCAGCTCTGAAAAGGTCCTCCCAGTCCCGCTCTTCTGCAT CGTGGCCACCGCTGTGATGTGGGCTGCCGCCCTATATTTTTTCTTCCAGAATCTCAGCAGCTGGGAGGGA ACTCCGGCCGAATCCCGGGAGAAGAACCGCGAGTGCATTCTGCTGGATTTCTTCGATGACCATGACATCT GGCACTTCCTCTCTGCTACTGCTCTGTTTTTCTCATTCTTGGTTTTGTTAACTTTGGATGATGACCTTGA TGTGGTTCGGAGAGACCAGATCCCTGTCTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC224950 representing NM_017699
Red=Cloning site Green=Tags(s) MRGCLRLALLCALPWLLLAASPGHPAKSPRQPPAPRRDPFDAARGADFDHVYSGVVNLSTENIYSFNYTS QPDQVTAVRVYVNSSSENLNYPVLVVVRQQKEVLSWQVPLLFQGLYQRSYNYQEVSRTLCPSEATNETGP LQQLIFVDVASMAPLGAQYKLLVTKLKHFQLRTNVAFHFTASPSQPQYFLYKFPKDVDSVIIKVVSEMAY PCSVVSVQNIMCPVYDLDHNVEFNGVYQSMTKKAAITLQKKDFPGEQFFVVFVIKPEDYACGGSFFIQEK ENQTWNLQRKKNLEVTIVPSIKESVYVKSSLFSVFIFLSFYLGCLLVGFVHYLRFQRKSIDGSFGSNDGS GNMVASHPIAASTPEGSNYGTIDESSSSPGRQMSSSDGGPPGQSDTDSSVEESDFDTMPDIESDKNIIRT KMFLYLSDLSRKDRRIVSKKYKIYFWNIITIAVFYALPVIQLVITYQTVVNVTGNQDICYYNFLCAHPLG VLSAFNNILSNLGHVLLGFLFLLIVLRRDILHRRALEAKDIFAVEYGIPKHFGLFYAMGIALMMEGVLSA CYHVCPNYSNFQFDTSFMYMIAGLCMLKLYQTRHPDINASAYSAYASFAVVIMVTVLGVVFGKNDVWFWV IFSAIHVLASLALSTQIYYMGRFKIDLGIFRRAAMVFYTDCIQQCSRPLYMDRMVLLVVGNLVNWSFALF GLIYRPRDFASYMLGIFICNLLLYLAFYIIMKLRSSEKVLPVPLFCIVATAVMWAAALYFFFQNLSSWEG TPAESREKNRECILLDFFDDHDIWHFLSATALFFSFLVLLTLDDDLDVVRRDQIPVF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_017699 |
ORF Size | 2481 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_017699.3 |
RefSeq Size | 5021 bp |
RefSeq ORF | 2484 bp |
Locus ID | 54847 |
UniProt ID | Q9NXL6 |
Protein Families | Transmembrane |
MW | 93.7 kDa |
Gene Summary | The protein encoded by this gene belongs to SID1 family of transmembrane dsRNA-gated channels. Family members transport dsRNA into cells and are required for systemic RNA interference. [provided by RefSeq, May 2017] |
Citations (2)
The use of this cDNA Clones has been cited in the following citations: |
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A C. elegans model of human a1-antitrypsin deficiency links components of the RNAi pathway to misfolded protein turnover
,Long, OS;Benson, JA;Kwak, JH;Luke, CJ;Gosai, SJ;O'Reilly, LP;Wang, Y;Li, J;Vetica, AC;Miedel, MT;Stolz, DB;Watkins, SC;Züchner, S;Perlmutter, DH;Silverman, GA;Pak, SC;,
Hum. Mol. Genet.
,PubMed ID 24838286
[SIDT1]
|
Homo sapiens Systemic RNA Interference-defective-1 Transmembrane Family Member 1 (SIDT1) Protein Mediates Contact-dependent Small RNA Transfer and MicroRNA-21-driven Chemoresistance
,Mohamed O. Elhassan, Jennifer Christie, and Mark S. Duxbury,
J. Biol. Chem., Feb 2012; 287: 5267 - 5277. J. Biol. Chem., Feb 2012; 287: 5267 - 5277.
[SIDT1]
|
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC224950L1 | Lenti ORF clone of Human SID1 transmembrane family, member 1 (SIDT1), Myc-DDK-tagged |
CNY 8,456.00 |
|
RC224950L2 | Lenti ORF clone of Human SID1 transmembrane family, member 1 (SIDT1), mGFP tagged |
CNY 7,700.00 |
|
RC224950L3 | Lenti ORF clone of Human SID1 transmembrane family, member 1 (SIDT1), Myc-DDK-tagged |
CNY 7,700.00 |
|
RC224950L4 | Lenti ORF clone of Human SID1 transmembrane family, member 1 (SIDT1), mGFP tagged |
CNY 7,700.00 |
|
RG224950 | SIDT1 (tGFP-tagged) - Human SID1 transmembrane family, member 1 (SIDT1) |
CNY 7,656.00 |
|
SC316839 | SIDT1 (untagged)-Human SID1 transmembrane family, member 1 (SIDT1) |
CNY 6,064.00 |