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SGLT2 (SLC5A2) (NM_003041) Human Tagged ORF Clone
CAT＃: RC224822
SLC5A2 (Myc-DDK-tagged)-Human solute carrier family 5 (sodium/glucose cotransporter), member 2 (SLC5A2)
ORF Plasmid: tGFP
"NM_003041" in other vectors (6)
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CNY 4,920.00
CNY 7,130.00
| Cited in 4 publications. |
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CNY 300.00
热销推荐Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | SGLT2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC224822 representing NM_003041
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGAGCACACAGAGGCAGGCTCGGCACCAGAGATGGGGGCCCAGAAGGCCCTGATTGACAATCCTG CTGACATCCTAGTCATTGCTGCATATTTCCTGCTGGTCATTGGCGTTGGCTTGTGGTCCATGTGCAGAAC CAACAGAGGCACTGTGGGCGGCTACTTCCTGGCAGGACGCAGCATGGTGTGGTGGCCGGTTGGGGCCTCT CTCTTCGCCAGCAACATCGGCAGTGGCCACTTTGTGGGCCTGGCAGGGACTGGCGCTGCAAGTGGCTTGG CTGTTGCTGGATTCGAGTGGAATGCGCTCTTCGTGGTGCTGCTACTGGGCTGGCTGTTTGCACCCGTGTA CCTGACAGCGGGGGTCATCACGATGCCACAGTACCTGCGCAAGCGCTTCGGCGGCCGCCGCATCCGCCTC TACCTGTCTGTGCTCTCCCTTTTCCTGTACATCTTCACCAAGATCTCAGTGGACATGTTCTCCGGAGCTG TATTCATCCAGCAGGCTCTGGGCTGGAACATCTATGCCTCCGTCATCGCGCTTCTGGGCATCACCATGAT TTACACGGTGACAGGAGGGCTGGCCGCGCTGATGTACACGGACACGGTACAGACCTTCGTCATTCTGGGG GGCGCCTGCATCCTCATGGGTTACGCCTTCCACGAGGTGGGCGGGTATTCGGGTCTCTTCGACAAATACC TGGGAGCAGCGACTTCGCTGACGGTGTCCGAGGATCCAGCCGTGGGAAACATCTCCAGCTTCTGCTATCG ACCCCGGCCCGACTCCTACCACCTGCTCCGGCACCCCGTGACCGGGGATCTGCCGTGGCCCGCGCTGCTC CTCGGACTCACAATCGTCTCGGGCTGGTACTGGTGCAGCGACCAGGTCATCGTGCAGCGCTGCCTGGCCG GGAAGAGCCTGACCCACATCAAGGCGGGCTGCATCCTGTGTGGGTACCTGAAGCTGACGCCCATGTTTCT CATGGTCATGCCAGGCATGATCAGCCGCATTCTGTACCCAGACGAGGTGGCGTGCGTGGTGCCTGAGGTG TGCAGGCGCGTGTGCGGCACGGAGGTGGGCTGCTCCAACATCGCCTACCCGCGGCTCGTCGTGAAGCTCA TGCCCAACGGTCTGCGCGGACTCATGCTGGCGGTCATGCTGGCCGCGCTCATGTCCTCGCTGGCCTCCAT CTTCAACAGCAGCAGCACGCTCTTCACCATGGACATCTACACGCGCCTGCGGCCACGCGCCGGCGACCGC GAGCTGCTGCTGGTGGGACGGCTCTGGGTGGTGTTCATCGTGGTAGTGTCGGTGGCCTGGCTTCCCGTGG TGCAGGCGGCACAGGGCGGGCAGCTCTTCGATTACATCCAGGCAGTCTCTAGCTACCTGGCACCGCCCGT GTCCGCCGTCTTCGTGCTGGCGCTCTTCGTGCCGCGCGTTAATGAGCAGGGCGCCTTCTGGGGACTCATC GGGGGCCTGCTGATGGGCCTGGCACGCCTGATTCCCGAGTTCTCCTTCGGCTCGGGCAGCTGTGTGCAGC CCTCGGCGTGCCCAGCTTTCCTCTGCGGCGTGCACTACCTCTACTTCGCCATTGTGCTGTTCTTCTGCTC TGGCCTCCTCACCCTCACGGTCTCCCTGTGCACCGCGCCCATCCCCAGAAAGCACCTCCACCGCCTGGTC TTCAGTCTCCGGCATAGCAAGGAGGAACGGGAGGACCTGGATGCTGATGAGCAGCAAGGCTCCTCACTCC CTGTACAGAATGGGTGCCCAGAGAGTGCCATGGAGATGAATGAGCCCCAGGCCCCGGCACCAAGCCTCTT CCGCCAGTGCCTGCTCTGGTTTTGTGGAATGAGCAGAGGTGGGGTGGGCAGTCCTCCGCCCCTTACCCAG GAGGAGGCAGCGGCAGCAGCCAGGCGGCTGGAGGACATCAGCGAGGACCCGAGCTGGGCCCGTGTGGTCA ACCTCAATGCCCTGCTCATGATGGCAGTGGCCGTGTTCCTCTGGGGCTTCTATGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC224822 representing NM_003041
Red=Cloning site Green=Tags(s) MEEHTEAGSAPEMGAQKALIDNPADILVIAAYFLLVIGVGLWSMCRTNRGTVGGYFLAGRSMVWWPVGAS LFASNIGSGHFVGLAGTGAASGLAVAGFEWNALFVVLLLGWLFAPVYLTAGVITMPQYLRKRFGGRRIRL YLSVLSLFLYIFTKISVDMFSGAVFIQQALGWNIYASVIALLGITMIYTVTGGLAALMYTDTVQTFVILG GACILMGYAFHEVGGYSGLFDKYLGAATSLTVSEDPAVGNISSFCYRPRPDSYHLLRHPVTGDLPWPALL LGLTIVSGWYWCSDQVIVQRCLAGKSLTHIKAGCILCGYLKLTPMFLMVMPGMISRILYPDEVACVVPEV CRRVCGTEVGCSNIAYPRLVVKLMPNGLRGLMLAVMLAALMSSLASIFNSSSTLFTMDIYTRLRPRAGDR ELLLVGRLWVVFIVVVSVAWLPVVQAAQGGQLFDYIQAVSSYLAPPVSAVFVLALFVPRVNEQGAFWGLI GGLLMGLARLIPEFSFGSGSCVQPSACPAFLCGVHYLYFAIVLFFCSGLLTLTVSLCTAPIPRKHLHRLV FSLRHSKEEREDLDADEQQGSSLPVQNGCPESAMEMNEPQAPAPSLFRQCLLWFCGMSRGGVGSPPPLTQ EEAAAAARRLEDISEDPSWARVVNLNALLMMAVAVFLWGFYA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003041 |
| ORF Size | 2016 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_003041.4 |
| RefSeq Size | 2271 bp |
| RefSeq ORF | 2019 bp |
| Locus ID | 6524 |
| UniProt ID | P31639 |
| Protein Families | Transmembrane |
| MW | 72.7 kDa |
| Gene Summary | This gene encodes a member of the sodium glucose cotransporter family which are sodium-dependent glucose transport proteins. The encoded protein is the major cotransporter involved in glucose reabsorption in the kidney. Mutations in this gene are associated with renal glucosuria. Two transcript variants, one protein-coding and one not, have been found for this gene. [provided by RefSeq, Feb 2015] |
Citations (4)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Pharmacological evaluation of HM41322, a novel SGLT1/2 dual inhibitor, in vitro and in vivo
,Lee, KH;Lee, SD;Kim, N;Suh, KH;Kim, YH;Sim, SS;,
Korean J. Physiol. Pharmacol.
,PubMed ID 30627010
[SGLT2]
|
|
Concise and Stereodivergent Synthesis of Carbasugars Reveals Unexpected Structure-Activity Relationship (SAR) of SGLT2 Inhibition
,Ng, WL;Li, HC;Lau, KM;Chan, AKN;Lau, CB;Shing, TKM;,
Sci Rep
,PubMed ID 28717146
[SGLT2]
|
|
Targeting Type 2 Diabetes with C-Glucosyl Dihydrochalcones as Selective Sodium Glucose Co-Transporter 2 (SGLT2) Inhibitors: Synthesis and Biological Evaluation
,Jesus, AR;Vila-Viçosa, D;Machuqueiro, M;Marques, AP;Dore, TM;Rauter, AP;,
Journal of Medicinal Chemistry
,PubMed ID 28098449
[SGLT2]
|
|
SHR3824, a novel selective inhibitor of renal sodium glucose cotransporter 2, exhibits antidiabetic efficacy in rodent models
,Yan, PK;Zhang, LN;Feng, Y;Qu, H;Qin, L;Zhang, LS;Leng, Y;,
Acta Pharmacol. Sin.
,PubMed ID 24786232
[SGLT2]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC224822L1 | Lenti ORF clone of Human solute carrier family 5 (sodium/glucose cotransporter), member 2 (SLC5A2), Myc-DDK-tagged |
CNY 7,320.00 |
|
| RC224822L2 | Lenti ORF clone of Human solute carrier family 5 (sodium/glucose cotransporter), member 2 (SLC5A2), mGFP tagged |
CNY 9,030.00 |
|
| RC224822L3 | Lenti ORF clone of Human solute carrier family 5 (sodium/glucose cotransporter), member 2 (SLC5A2), Myc-DDK-tagged |
CNY 7,320.00 |
|
| RC224822L4 | Lenti ORF clone of Human solute carrier family 5 (sodium/glucose cotransporter), member 2 (SLC5A2), mGFP tagged |
CNY 7,320.00 |
|
| RG224822 | SLC5A2 (tGFP-tagged) - Human solute carrier family 5 (sodium/glucose cotransporter), member 2 (SLC5A2) |
CNY 6,520.00 |
|
| SC303267 | SLC5A2 (untagged)-Human solute carrier family 5 (sodium/glucose cotransporter), member 2 (SLC5A2) |
CNY 4,928.00 |
