GPSM1 (NM_015597) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AGS3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC224368 representing NM_015597 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGGCCCGGCCCCGCCCGTGGCCGACGAGCTCCCGGGCCCGGCCGCCAGGCGCCTCTACTCCAGGA TGGAGGCGTCCTGCCTAGAGCTGGCGCTGGAGGGCGAGCGTCTGTGCAAGGCGGGCGACTTCAAGACAGG CGTGGCCTTCTTTGAGGCTGCTGTGCAGGTGGGCACCGAGGACCTGAAGACACTGAGTGCCATCTACAGC CAGCTGGGCAACGCCTACTTCTACCTGAAGGAGCACGGCCGGGCGCTGGAATACCACAAGCATGACCTCC TGCTGGCGCGGACCATCGGTGACCGCATGGGGGAGGCCAAGGCCAGTGGAAACCTGGGAAACACACTCAA GGTCCTGGGGCGCTTCGACGAGGCTGCCGTCTGCTGCCAGCGGCATCTGAGCATCGCCCAAGAGCAGGGA GACAAGGTTGGGGAGGCGAGGGCCCTCTACAACATCGGGAACGTGTACCACGCCAAAGGCAAGCAACTGT CCTGGAACGCCGCAAACGCCACGCAGGACCCCGGGCACCTGCCGCCCGATGTCCGAGAGACCCTGTGCAA GGCCTCCGAGTTCTACGAGAGGAACCTGTCCCTGGTGAAGGAGCTGGGCGACCGTGCGGCGCAGGGCAGG GCCTACGGCAACCTGGGCAACACCCACTATTTGTTGGGGAACTTCACAGAGGCCACGACCTTCCACAAGG AGCGCCTGGCCATTGCTAAGGAGTTTGGAGACAAGGCAGCCGAGAGGAGGGCCTACAGCAACCTGGGGAA CGCCCACGTCTTCCTGGGGCGCTTTGACGTGGCCGCCGAGTACTACAAGAAGACGCTGCAACTGTCTCGG CAGCTCAGGGACCAGGCAGTGGAGGCGCAGGCCTGCTACAGTCTGGGCAACACCTACACGCTGCTGCAGG ACTACGAGCGCGCGGCCGAGTACCACCTGCGGCACCTGCTCATTGCCCAGGAGCTGGCCGACAGAGTGGG CGAGGGCCGGGCGTGCTGGAGCCTGGGAAATGCCTACGTGTCCATGGGGCGCCCAGCGCAGGCCCTGACC TTCGCCAAGAAGCACCTGCAGATCTCCCAGGAGATCGGGGACCGCCATGGGGAGCTCACGGCCCGCATGA ACGTGGCGCAGCTGCAGCTGGTGCTCGGCCGCCTGACCAGCCCGGCAGCCTCAGAGAAGCCTGACCTGGC CGGCTATGAGGCCCAGGGTGAGTTCCAGGGTTGTGGGGGGGTCTTGCTCCCCACAGGCACGGACCGCATC AGGAGCTGCGGAGGGGTGGGATCGAGGCCAGGCCAGCATGGCGGAGGTGGCAGCCGCCAGAAAATGGCGC CTACAAGCCAGTTCTTCTTGGCCTCAGGGACAGCACAGGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC224368 representing NM_015597 Red=Cloning site Green=Tags(s) MAGPAPPVADELPGPAARRLYSRMEASCLELALEGERLCKAGDFKTGVAFFEAAVQVGTEDLKTLSAIYS QLGNAYFYLKEHGRALEYHKHDLLLARTIGDRMGEAKASGNLGNTLKVLGRFDEAAVCCQRHLSIAQEQG DKVGEARALYNIGNVYHAKGKQLSWNAANATQDPGHLPPDVRETLCKASEFYERNLSLVKELGDRAAQGR AYGNLGNTHYLLGNFTEATTFHKERLAIAKEFGDKAAERRAYSNLGNAHVFLGRFDVAAEYYKKTLQLSR QLRDQAVEAQACYSLGNTYTLLQDYERAAEYHLRHLLIAQELADRVGEGRACWSLGNAYVSMGRPAQALT FAKKHLQISQEIGDRHGELTARMNVAQLQLVLGRLTSPAASEKPDLAGYEAQGEFQGCGGVLLPTGTDRI RSCGGVGSRPGQHGGGGSRQKMAPTSQFFLASGTAQA TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_015597
ORF Size	1371 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_015597.4, NP_056412.4
RefSeq Size	2282 bp
RefSeq ORF	1374 bp
Locus ID	26086
UniProt ID	Q86YR5
MW	49.7 kDa
Gene Summary	G-protein signaling modulators (GPSMs) play diverse functional roles through their interaction with G-protein subunits. This gene encodes a receptor-independent activator of G protein signaling, which is one of several factors that influence the basal activity of G-protein signaling systems. The protein contains seven tetratricopeptide repeats in its N-terminal half and four G-protein regulatory (GPR) motifs in its C-terminal half. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]

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