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SPTLC3 (NM_018327) Human Tagged ORF Clone
CAT＃: RC224216
- TrueORF®
SPTLC3 (Myc-DDK-tagged)-Human serine palmitoyltransferase, long chain base subunit 3 (SPTLC3)
ORF Plasmid: tGFP
"NM_018327" in other vectors (5)
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CNY 4,112.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | C20orf38; dJ718P11; dJ718P11.1; hLCB2b; LCB2B; LCB 3; LCB3; SPT 3; SPT3; SPTLC2L |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC224216 representing NM_018327
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTAACCCTGGAGGTGGTGCTGTTTGCAACGGGAAACTTCACAATCACAAGAAACAGAGCAATGGCT CACAAAGCAGAAACTGCACAAAGAATGGAATAGTGAAGGAAGCCCAGCAAAATGGGAAGCCACATTTTTA TGATAAGCTCATTGTTGAATCGTTTGAGGAAGCACCCCTTCATGTTATGGTTTTCACTTACATGGGATAT GGAATTGGAACCCTGTTTGGCTATCTCAGAGACTTTTTAAGAAACTGGGGAATAGAAAAATGCAACGCAG CTGTGGAACGAAAAGAACAAAAAGATTTTGTGCCACTGTATCAAGACTTTGAAAATTTTTATACAAGAAA CCTTTACATGCGAATCAGAGACAACTGGAACCGGCCCATCTGCAGTGCCCCAGGGCCTCTGTTTGATGTG ATGGAGAGGGTATCGGACGACTATAACTGGACGTTTAGGTTTACTGGAAGAGTCATCAAAGATGTCATCA ACATGGGCTCCTATAACTTCCTTGGTCTTGCAGCCAAGTATGATGAGTCTATGAGGACAATAAAGGATGT TTTAGAGGTGTATGGCACAGGCGTGGCCAGCACCAGGCATGAAATGGGCACCTTGGATAAGCACAAGGAG TTGGAGGACCTTGTGGCTAAGTTCCTGAATGTGGAAGCAGCTATGGTCTTTGGGATGGGATTCGCAACTA ACTCAATGAATATCCCAGCATTAGTTGGAAAGGGATGCCTCATTTTAAGTGATGAGTTAAACCACACATC GCTTGTGCTTGGGGCCCGACTCTCAGGTGCAACCATAAGAATCTTCAAACACAACAACACACAAAGCCTA GAGAAGCTCCTGAGAGATGCTGTCATCTATGGCCAGCCTCGAACCCGCAGAGCTTGGAAAAAGATTCTCA TCCTGGTGGAGGGTGTCTACAGCATGGAAGGTTCCATCGTGCATCTGCCCCAGATCATAGCTCTAAAGAA GAAATACAAGGCTTACCTCTACATAGATGAAGCTCACAGTATTGGGGCCGTGGGCCCAACCGGCCGGGGT GTCACGGAGTTCTTTGGACTAGACCCTCATGAAGTTGATGTGCTCATGGGCACATTCACCAAAAGTTTTG GAGCTTCAGGAGGTTACATAGCTGGAAGGAAGGACCTCGTGGATTATTTACGGGTTCACTCGCATAGTGC TGTTTATGCTTCATCCATGAGCCCACCGATAGCAGAGCAAATCATCAGATCACTAAAACTTATCATGGGA CTGGATGGGACCACTCAAGGGCTGCAGAGAGTACAGCAACTTGCGAAAAACACAAGATACTTCAGACAAA GACTGCAGGAAATGGGATTCATTATCTATGGCAATGAGAATGCTTCTGTTGTTCCTCTGCTTCTTTATAT GCCTGGTAAAGTAGCGGCTTTTGCAAGGCATATGCTAGAGAAAAAAATTGGAGTGGTGGTCGTGGGATTT CCAGCCACTCCCCTCGCAGAAGCTCGGGCTCGGTTTTGTGTTTCAGCGGCACATACCCGGGAGATGTTAG ACACGGTTTTAGAAGCTCTTGATGAAATGGGTGATCTCTTGCAACTGAAATATTCCCGGCACAAGAAGTC AGCACGTCCTGAGCTCTATGATGAGACGAGCTTTGAACTCGAAGAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC224216 representing NM_018327
Red=Cloning site Green=Tags(s) MANPGGGAVCNGKLHNHKKQSNGSQSRNCTKNGIVKEAQQNGKPHFYDKLIVESFEEAPLHVMVFTYMGY GIGTLFGYLRDFLRNWGIEKCNAAVERKEQKDFVPLYQDFENFYTRNLYMRIRDNWNRPICSAPGPLFDV MERVSDDYNWTFRFTGRVIKDVINMGSYNFLGLAAKYDESMRTIKDVLEVYGTGVASTRHEMGTLDKHKE LEDLVAKFLNVEAAMVFGMGFATNSMNIPALVGKGCLILSDELNHTSLVLGARLSGATIRIFKHNNTQSL EKLLRDAVIYGQPRTRRAWKKILILVEGVYSMEGSIVHLPQIIALKKKYKAYLYIDEAHSIGAVGPTGRG VTEFFGLDPHEVDVLMGTFTKSFGASGGYIAGRKDLVDYLRVHSHSAVYASSMSPPIAEQIIRSLKLIMG LDGTTQGLQRVQQLAKNTRYFRQRLQEMGFIIYGNENASVVPLLLYMPGKVAAFARHMLEKKIGVVVVGF PATPLAEARARFCVSAAHTREMLDTVLEALDEMGDLLQLKYSRHKKSARPELYDETSFELED TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_018327 |
| ORF Size | 1656 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_018327.4 |
| RefSeq Size | 3855 bp |
| RefSeq ORF | 1659 bp |
| Locus ID | 55304 |
| UniProt ID | Q9NUV7 |
| MW | 61.9 kDa |
| Gene Summary | This gene encodes a subunit of the serine palmitoyltransferase complex which catalyzes the rate-limiting step in sphingolipid biosynthesis. This subunit metabolizes lauroyl- and myristoyl-CoA and generates C14 and C16-sphingoid bases. [provided by RefSeq, Mar 2017] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC224216L3 | Lenti ORF clone of Human serine palmitoyltransferase, long chain base subunit 3 (SPTLC3), Myc-DDK-tagged |
CNY 6,512.00 |
|
| RC224216L4 | Lenti ORF clone of Human serine palmitoyltransferase, long chain base subunit 3 (SPTLC3), mGFP tagged |
CNY 6,512.00 |
|
| RG224216 | SPTLC3 (tGFP-tagged) - Human serine palmitoyltransferase, long chain base subunit 3 (SPTLC3) |
CNY 5,712.00 |
|
| SC113546 | SPTLC3 (untagged)-Human serine palmitoyltransferase, long chain base subunit 3 (SPTLC3) |
CNY 7,220.00 |
|
| SC317943 | SPTLC3 (untagged)-Human serine palmitoyltransferase, long chain base subunit 3 (SPTLC3) |
CNY 7,220.00 |
