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GPR133 (ADGRD1) (NM_198827) Human Tagged ORF Clone
CAT＃: RC224123
GPR133 (Myc-DDK-tagged)-Human G protein-coupled receptor 133 (GPR133)
ORF Plasmid: tGFP
"NM_198827" in other vectors (6)
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CNY 6,400.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | GPR133; PGR25 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC224123 representing NM_198827
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAAAGCTGCTGCGGCTGTGCTGCTGGTACTCCTGGCTGCTGCTATTTTATTACAACTTTCAGGTGC GTGGCGTCTACTCCAGATCGCAGGACCATCCAGGATTTCAGGTGTTGGCGTCTGCTTCCCATTACTGGCC ACTGGAGAATGTGGATGGGATCCATGAACTTCAGGATACAACTGGAGATATTGTGGAAGGGAAGGTCAAC AAAGGCATTTACCTGAAAGAGGAAAAGGGAGTCACGCTTCTCTATTACGGCAGGTACAACAGCTCCTGCA TCAGCAAGCCAGAGCAGTGTGGCCCTGAAGGGGTCACGTTTTCTTTTTTCTGGAAGACACAAGGAGAACA GTCTAGACCAATCCCTTCTGCGTATGGGGGACAGGTCATCTCCAATGGGTTCAAAGTCTGCTCCAGCGGT GGCAGAGGCTCTGTGGAGCTGTACACGCGGGACAATTCCATGACATGGGAGGCCTCCTTCAGCCCCCCAG GCCCCTATTGGACTCATGTCCTATTTACATGGAAATCCAAGGAGGGCCTGAAAGTCTACGTCAACGGGAC CCTGAGCACCTCTGATCCGAGTGGAAAAGTGTCTCGTGACTATGGAGAGTCCAACGTCAACCTCGTGATA GGGTCTGAGCAGGACCAGGCCAAGTGTTATGAGAACGGTGCTTTCGATGAGTTCATCATCTGGGAGCGGG CTCTGACTCCGGATGAGATCGCCATGTACTTCACTGCTGCCATTGGAAAGCATGCTTTATTGTCTTCAAC GCTGCCAAGCCTCTTCATGACATCCACAGCAAGCCCCGTGATGCCCACAGATGCCTACCATCCCATCATA ACCAACCTGACAGAAGAGAGAAAAACCTTCCAAAGTCCCGGAGTGATACTGAGTTACCTCCAAAATGTAT CCCTCAGCTTACCCAGTAAGTCCCTCTCGGAGCAGACAGCCTTGAATCTCACCAAGACCTTCTTAAAAGC CGTGGGAGAGATCCTTCTACTGCCTGGTTGGATTGCTCTGTCAGAGGACAGCGCCGTGGTACTGAGTCTC ATCGACACTATTGACACCGTCATGGGCCATGTATCCTCCAACCTGCACGGCAGCACGCCCCAGGTCACCG TGGAGGGCTCCTCTGCCATGGCAGAGTTTTCCGTGGCCAAAATCCTGCCCAAGACCGTGAATTCCTCCCA TTACCGCTTCCCGGCCCACGGGCAGAGCTTCATCCAGATCCCCCACGAGGCCTTCCACAGGCACGCCTGG AGCACCGTCGTGGGTCTGCTGTACCACAGCATGCACTACTACCTGAACAACATCTGGCCCGCCCACACCA AGATCGCGGAGGCCATGCATCACCAGGACTGCCTGCTGTTCGCCACCAGCCACCTGATTTCCCTGGAGGT GTCCCCACCACCCACCCTGTCTCAGAACCTGTCGGGCTCTCCACTCATTACGGTCCACCTCAAGCACAGA TTGACACGTAAGCAGCACAGTGAGGCCACCAACAGCAGCAACCGAGTCTTCGTGTACTGCGCCTTCCTGG ACTTCAGCTCCGGAGAAGGGGTCTGGTCGAACCACGGCTGTGCGCTCACGAGAGGAAACCTCACCTACTC CGTCTGCCGCTGCACTCACCTCACCAACTTTGCCATCCTCATGCAGGTGGTCCCGCTGGAGCTTGCACGC GGACACCAGGTGGCGCTGTCGTCTATCAGCTATGTGGGCTGCTCCCTCTCCGTGCTCTGCCTGGTGGCCA CGCTGGTCACCTTCGCCGTGCTGTCCTCCGTGAGCACCATCCGGAACCAGCGCTACCACATCCACGCCAA CCTGTCCTTCGCCGTGCTGGTGGCCCAGGTCCTGCTGCTCATTAGTTTCCGCCTCGAGCCGGGCACGACC CCCTGCCAAGTGATGGCCGTGCTCCTACACTACTTCTTCCTGAGTGCCTTCGCATGGATGCTGGTGGAGG GGCTGCACCTCTACAGCATGGTGATCAAGGTCTTTGGGTCGGAGGACAGCAAGCACCGTTACTACTATGG GATGGGATGGGGTTTTCCTCTTCTGATCTGCATCATTTCACTGTCATTTGCCATGGACAGTTACGGAACA AGCAACAATTGCTGGCTGTCGTTGGCGAGTGGCGCCATCTGGGCCTTTGTAGCCCCTGCTCTGTTTGTCA TCGTGGTCAACATTGGCATCCTCATCGCTGTGACCAGAGTCATCTCACAGATCAGCGCCGACAACTACAA GATCCATGGAGACCCCAGTGCCTTCAAGTTGACAGCCAAGGCAGTGGCCGTGCTGCTGCCCATCCTGGGT ACCTCGTGGGTCTTTGGCGTGCTTGCTGTCAACGGTTGTGCTGTGGTTTTCCAGTACATGTTTGCCACGC TCAACTCCCTGCAGGGACTGTTCATATTCCTCTTTCATTGTCTCCTGAATTCAGAGGTGAGAGCCGCCTT CAAGCACAAAACCAAGGTCTGGTCGCTCACGAGCAGCTCCGCCCGCACCTCCAACGCGAAGCCCTTCCAC TCGGACCTCATGAATGGGACCCGGCCAGGCATGGCCTCCACCAAGCTCAGCCCTTGGGACAAGAGCAGCC ACTCTGCCCACCGCGTCGACCTGTCAGCCGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC224123 representing NM_198827
Red=Cloning site Green=Tags(s) MEKLLRLCCWYSWLLLFYYNFQVRGVYSRSQDHPGFQVLASASHYWPLENVDGIHELQDTTGDIVEGKVN KGIYLKEEKGVTLLYYGRYNSSCISKPEQCGPEGVTFSFFWKTQGEQSRPIPSAYGGQVISNGFKVCSSG GRGSVELYTRDNSMTWEASFSPPGPYWTHVLFTWKSKEGLKVYVNGTLSTSDPSGKVSRDYGESNVNLVI GSEQDQAKCYENGAFDEFIIWERALTPDEIAMYFTAAIGKHALLSSTLPSLFMTSTASPVMPTDAYHPII TNLTEERKTFQSPGVILSYLQNVSLSLPSKSLSEQTALNLTKTFLKAVGEILLLPGWIALSEDSAVVLSL IDTIDTVMGHVSSNLHGSTPQVTVEGSSAMAEFSVAKILPKTVNSSHYRFPAHGQSFIQIPHEAFHRHAW STVVGLLYHSMHYYLNNIWPAHTKIAEAMHHQDCLLFATSHLISLEVSPPPTLSQNLSGSPLITVHLKHR LTRKQHSEATNSSNRVFVYCAFLDFSSGEGVWSNHGCALTRGNLTYSVCRCTHLTNFAILMQVVPLELAR GHQVALSSISYVGCSLSVLCLVATLVTFAVLSSVSTIRNQRYHIHANLSFAVLVAQVLLLISFRLEPGTT PCQVMAVLLHYFFLSAFAWMLVEGLHLYSMVIKVFGSEDSKHRYYYGMGWGFPLLICIISLSFAMDSYGT SNNCWLSLASGAIWAFVAPALFVIVVNIGILIAVTRVISQISADNYKIHGDPSAFKLTAKAVAVLLPILG TSWVFGVLAVNGCAVVFQYMFATLNSLQGLFIFLFHCLLNSEVRAAFKHKTKVWSLTSSSARTSNAKPFH SDLMNGTRPGMASTKLSPWDKSSHSAHRVDLSAV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_198827 |
| ORF Size | 2622 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_198827.5 |
| RefSeq Size | 2823 bp |
| RefSeq ORF | 2625 bp |
| Locus ID | 283383 |
| UniProt ID | Q6QNK2 |
| Protein Families | Druggable Genome, Transmembrane |
| MW | 96.3 kDa |
| Gene Summary | The adhesion G-protein-coupled receptors (GPCRs), including GPR133, are membrane-bound proteins with long N termini containing multiple domains. GPCRs, or GPRs, contain 7 transmembrane domains and transduce extracellular signals through heterotrimeric G proteins (summary by Bjarnadottir et al., 2004 [PubMed 15203201]).[supplied by OMIM, Nov 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC224123L1 | Lenti ORF clone of Human G protein-coupled receptor 133 (GPR133), Myc-DDK-tagged |
CNY 8,800.00 |
|
| RC224123L2 | Lenti ORF clone of Human G protein-coupled receptor 133 (GPR133), mGFP tagged |
CNY 5,890.00 |
|
| RC224123L3 | Lenti ORF clone of Human G protein-coupled receptor 133 (GPR133), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC224123L4 | Lenti ORF clone of Human G protein-coupled receptor 133 (GPR133), mGFP tagged |
CNY 5,890.00 |
|
| RG224123 | GPR133 (tGFP-tagged) - Human G protein-coupled receptor 133 (GPR133) |
CNY 4,370.00 |
|
| SC307786 | GPR133 (untagged)-Human G protein-coupled receptor 133 (GPR133) |
CNY 6,408.00 |
