HEPACAM (NM_152722) Human Tagged ORF Clone

CAT#: RC223693

HEPACAM (Myc-DDK-tagged)-Human hepatic and glial cell adhesion molecule (HEPACAM)

ORF Plasmid: DDK tGFP



  "NM_152722" in other vectors (4)

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CNY 3,656.00

CNY 3,990.00


货期*
2周

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Rabbit polyclonal Anti-HEPACAM Antibody
    • 50 ug

CNY 5,250.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms GlialCAM; HEPN1; MLC2A; MLC2B
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC223693 representing NM_152722.
Blue=ORF Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGAAGAGAGAAAGGGGAGCCCTGTCCAGAGCCTCCAGGGCCCTGCGCCTTGCTCCTTTTGTCTACCTT
CTTCTGATCCAGACAGACCCCCTGGAGGGGGTGAACATCACCAGCCCCGTGCGCCTGATCCATGGCACC
GTGGGGAAGTCGGCTCTGCTTTCTGTGCAGTACAGCAGTACCAGCAGCGACAGGCCTGTAGTGAAGTGG
CAGCTGAAGCGGGACAAGCCAGTGACCGTGGTGCAGTCCATTGGCACAGAGGTCATCGGCACCCTGCGG
CCTGACTATCGAGACCGTATCCGACTCTTTGAAAATGGCTCCCTGCTTCTCAGCGACCTGCAGCTGGCC
GATGAGGGCACCTATGAGGTCGAGATCTCCATCACCGACGACACCTTCACTGGGGAGAAGACCATCAAC
CTTACTGTAGATGTGCCCATTTCGAGGCCACAGGTGTTGGTGGCTTCAACCACTGTGCTGGAGCTCAGC
GAGGCCTTCACCTTGAACTGCTCACATGAGAATGGCACCAAGCCCAGCTACACCTGGCTGAAGGATGGC
AAGCCCCTCCTCAATGACTCGAGAATGCTCCTGTCCCCCGACCAAAAGGTGCTCACCATCACCCGCGTG
CTCATGGAGGATGACGACCTGTACAGCTGCATGGTGGAGAACCCCATCAGCCAGGGCCGCAGCCTGCCT
GTCAAGATCACCGTATACAGAAGAAGCTCCCTTTACATCATCTTGTCTACAGGAGGCATCTTCCTCCTT
GTGACCTTGGTGACAGTCTGTGCCTGCTGGAAACCCTCCAAAAGGAAACAGAAGAAGCTAGAAAAGCAA
AACTCCCTGGAATACATGGATCAGAATGATGACCGCCTGAAACCAGAAGCAGACACCCTCCCTCGAAGT
GGTGAGCAGGAACGGAAGAACCCCATGGCACTCTATATCCTGAAGGACAAGGACTCCCCGGAGACCGAG
GAGAACCCGGCCCCGGAGCCTCGAAGCGCGACGGAGCCCGGCCCGCCCGGCTACTCCGTGTCTCCCGCC
GTGCCCGGCCGCTCGCCGGGGCTGCCCATCCGCTCTGCCCGCCGCTACCCGCGCTCCCCAGCGCGCTCC
CCAGCCACCGGCCGGACACACTCGTCGCCGCCCAGGGCCCCGAGCTCGCCCGGCCGCTCGCGCAGCGCC
TCGCGCACACTGCGGACTGCGGGCGTGCACATAATCCGCGAGCAAGACGAGGCCGGCCCGGTGGAGATC
AGCGCC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
>Peptide sequence encoded by RC223693
Blue=ORF Red=Cloning site Green=Tag(s)

MKRERGALSRASRALRLAPFVYLLLIQTDPLEGVNITSPVRLIHGTVGKSALLSVQYSSTSSDRPVVKW
QLKRDKPVTVVQSIGTEVIGTLRPDYRDRIRLFENGSLLLSDLQLADEGTYEVEISITDDTFTGEKTIN
LTVDVPISRPQVLVASTTVLELSEAFTLNCSHENGTKPSYTWLKDGKPLLNDSRMLLSPDQKVLTITRV
LMEDDDLYSCMVENPISQGRSLPVKITVYRRSSLYIILSTGGIFLLVTLVTVCACWKPSKRKQKKLEKQ
NSLEYMDQNDDRLKPEADTLPRSGEQERKNPMALYILKDKDSPETEENPAPEPRSATEPGPPGYSVSPA
VPGRSPGLPIRSARRYPRSPARSPATGRTHSSPPRAPSSPGRSRSASRTLRTAGVHIIREQDEAGPVEI
SA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV

Recombinant protein using RC223693 also available, TP323693
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_152722
ORF Size 1248 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_152722.5
RefSeq Size 3258 bp
RefSeq ORF 1251 bp
Locus ID 220296
UniProt ID Q14CZ8
Protein Families Druggable Genome, Transmembrane
MW 46 kDa
Gene Summary The protein encoded by this gene is a single-pass type I membrane protein that localizes to the cytoplasmic side of the cell membrane. The encoded protein acts as a homodimer and is involved in cell motility and cell-matrix interactions. The expression of this gene is downregulated or undetectable in many cancer cell lines, so this may be a tumor suppressor gene. [provided by RefSeq, Jul 2011]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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