ZNF248 (NM_021045) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	bA162G10.3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC223619 ORF sequence, codon optimized. Due to the complexity of NM_021045, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACAAGAGTCAGGAGCAGGTTTCCTTCAAGGACGTCTGTGTGGACTTCACGCAGGAAGAATGGTACC TTTTGGATCCTGCCCAGAAAATACTGTATAGGGACGTGATCCTGGAGAACTACTCAAATCTGGTAAGCGT AGGGTATTGCATCACAAAGCCGGAAGTTATTTTCAAGATAGAGCAGGGCGAGGAACCCTGGATCCTGGAG AAGGGCTTTCCATCACAGTGTCACCCAGAGCGGAAGTGGAAGGTTGATGACGTGTTGGAAAGCTCCCAGG AAAATGAAGACGATCATTTCTGGGAGCTTCTGTTCCACAATAACAAGACGGTTAGCGTGGAGAATGGCGA CAGAGGGAGTAAGACCTTTAACTTGGGGACTGACCCAGTGAGTCTCAGAAATTACCCTTATAAGATCTGT GACTCATGCGAAATGAATTTGAAAAACATCTCCGGCCTTATAATCTCTAAGAAGAACTGCTCACGGAAGA AGCCAGACGAGTTCAACGTGTGCGAGAAGTTGCTGCTGGATATAAGACACGAGAAAATCCCTATTGGAGA AAAGAGTTACAAGTACGACCAGAAGCGAAATGCAATTAACTACCACCAGGACCTGTCTCAGCCAAGTTTC GGCCAGTCTTTTGAATACAGCAAAAATGGACAGGGTTTTCACGACGAAGCCGCATTTTTTACCAACAAAC GAAGTCAGATTGGGGAAACAGTGTGTAAATATAACGAGTGCGGACGGACTTTTATCGAGTCCCTGAAATT GAATATATCTCAAAGACCACACCTGGAGATGGAGCCATACGGCTGTTCAATCTGTGGCAAGTCCTTTTGC ATGAACCTGAGATTTGGGCACCAACGCGCACTTACGAAAGACAACCCATACGAGTACAACGAGTACGGGG AGATATTCTGCGACAACAGTGCCTTCATTATTCATCAAGGTGCATACACCCGGAAGATTCTGAGAGAGTA CAAAGTAAGTGATAAGACCTGGGAGAAGAGCGCCTTGCTGAAGCACCAAATTGTCCACATGGGGGGTAAA TCTTACGATTACAACGAAAACGGAAGTAACTTTTCCAAGAAATCACACCTCACACAGCTTCGCCGGGCCC ACACCGGCGAGAAAACCTTTGAGTGTGGGGAGTGCGGCAAGACTTTCTGGGAGAAATCCAATCTGACTCA GCACCAACGGACTCATACCGGGGAGAAACCATATGAGTGTACGGAATGTGGAAAGGCATTCTGTCAGAAG CCCCACCTCACCAATCATCAGAGAACACATACAGGCGAGAAACCCTACGAATGTAAGCAGTGTGGAAAGA CATTTTGTGTGAAATCAAACCTCACTGAGCATCAAAGAACCCATACAGGGGAGAAGCCATACGAGTGCAA TGCATGCGGCAAATCCTTCTGTCACCGGAGCGCCCTGACAGTCCACCAGCGAACGCATACGGGTGAGAAA CCATTCATCTGTAACGAATGCGGCAAGTCATTCTGTGTGAAGTCAAATCTCATTGTCCACCAACGCACTC ACACGGGTGAAAAGCCATATAAATGCAACGAATGTGGCAAGACCTTTTGTGAGAAGTCCGCTCTCACCAA GCACCAGAGGACACACACCGGTGAGAAGCCCTACGAATGCAACGCTTGCGGAAAGACTTTTAGCCAGCGC TCAGTTCTTACAAAACATCAGCGAATCCACACCAGAGTGAAGGCCCTGTCTACGAGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC223619 representing NM_021045 Red=Cloning site Green=Tags(s) MNKSQEQVSFKDVCVDFTQEEWYLLDPAQKILYRDVILENYSNLVSVGYCITKPEVIFKIEQGEEPWILE KGFPSQCHPERKWKVDDVLESSQENEDDHFWELLFHNNKTVSVENGDRGSKTFNLGTDPVSLRNYPYKIC DSCEMNLKNISGLIISKKNCSRKKPDEFNVCEKLLLDIRHEKIPIGEKSYKYDQKRNAINYHQDLSQPSF GQSFEYSKNGQGFHDEAAFFTNKRSQIGETVCKYNECGRTFIESLKLNISQRPHLEMEPYGCSICGKSFC MNLRFGHQRALTKDNPYEYNEYGEIFCDNSAFIIHQGAYTRKILREYKVSDKTWEKSALLKHQIVHMGGK SYDYNENGSNFSKKSHLTQLRRAHTGEKTFECGECGKTFWEKSNLTQHQRTHTGEKPYECTECGKAFCQK PHLTNHQRTHTGEKPYECKQCGKTFCVKSNLTEHQRTHTGEKPYECNACGKSFCHRSALTVHQRTHTGEK PFICNECGKSFCVKSNLIVHQRTHTGEKPYKCNECGKTFCEKSALTKHQRTHTGEKPYECNACGKTFSQR SVLTKHQRIHTRVKALSTS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_021045
ORF Size	1737 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_021045.1, NM_021045.2, NP_066383.1
RefSeq Size	5191 bp
RefSeq ORF	1740 bp
Locus ID	57209
UniProt ID	Q8NDW4
Protein Families	Transcription Factors
MW	67.1 kDa
Gene Summary	May be involved in transcriptional regulation.[UniProtKB/Swiss-Prot Function]

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