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Semaphorin 3B (SEMA3B) (NM_004636) Human Tagged ORF Clone
CAT＃: RC223532
SEMA3B (Myc-DDK-tagged)-Human sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3B (SEMA3B), transcript variant 1
ORF Plasmid: tGFP
"NM_004636" in other vectors (4)
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CNY 5,488.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | LUCA-1; SemA; SEMA5; SEMAA; semaV |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC223532 representing NM_004636
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGCGGGCCGGGGCTGCCGCCGTGATCCCGGGCCTGGCCCTGCTCTGGGCAGTGGGGCTGGGGAGTG CCGCCCCCAGCCCCCCACGCCTTCGGCTCTCCTTCCAAGAGCTCCAGGCCTGGCATGGTCTCCAGACTTT CAGCCTGGAGCGAACCTGCTGCTACCAGGCCTTGCTGGTGGATGAGGAGCGTGGACGCCTGTTTGTGGGT GCCGAGAACCATGTGGCCTCCCTCAACCTGGACAACATCAGCAAGCGGGCCAAGAAGCTGGCCTGGCCGG CCCCTGTGGAATGGCGAGAGGAGTGCAACTGGGCAGGGAAGGACATTGGTACTGAGTGCATGAACTTCGT GAAGTTGCTGCATGCCTACAACCGCACCCATTTGCTGGCCTGTGGCACGGGAGCCTTCCACCCAACCTGT GCCTTTGTGGAAGTGGGCCACCGGGCAGAGGAGCCCGTCCTCCGGCTGGACCCAGGAAGGATAGAGGATG GCAAGGGGAAGAGTCCTTATGACCCCAGGCATCGGGCTGCCTCCGTGCTGGTGGGGGAGGAGCTATACTC AGGGGTGGCAGCAGACCTCATGGGACGAGACTTTACCATCTTTCGCAGCCTAGGGCAACGTCCAAGTCTC CGAACAGAGCCACACGACTCCCGCTGGCTCAATGAGCCCAAGTTTGTCAAGGTATTTTGGATCCCGGAGA GCGAGAACCCAGACGACGACAAAATCTACTTCTTCTTTCGTGAGACGGCGGTAGAGGCGGCGCCGGCACT GGGACGCCTGTCCGTGTCCCGCGTTGGCCAGATCTGCCGGAACGACGTGGGCGGCCAGCGCAGCCTGGTC AACAAGTGGACGACGTTCCTGAAGGCGCGGCTGGTGTGCTCGGTGCCCGGCGTCGAGGGCGACACCCACT TCGATCAGCTCCAGGATGTGTTTCTGTTGTCCTCGCGGGACCACCGGACCCCGCTGCTCTATGCCGTCTT CTCCACGTCCAGCAGCATCTTCCAGGGCTCTGCGGTGTGCGTGTACAGCATGAACGACGTGCGCCGGGCC TTCTTGGGACCCTTTGCACACAAGGAGGGGCCCATGCACCAGTGGGTGTCATACCAGGGTCGCGTCCCCT ACCCGCGGCCAGGCATGTGCCCCAGCAAGACCTTTGGCACCTTCAGTTCCACCAAGGACTTCCCAGACGA TGTCATCCAGTTTGCGCGGAACCACCCCCTCATGTACAACTCTGTCCTGCCCACTGGGGGGCGCCCTCTT TTCCTACAAGTTGGAGCCAATTACACCTTCACTCAAATTGCCGCGGACCGGGTTGCAGCCGCTGACGGAC ACTATGACGTCCTCTTCATTGGCACAGACGTTGGCACGGTGCTGAAGGTGATCTCGGTCCCCAAGGGCAG TAGGCCCAGCGCAGAGGGGCTGCTCCTGGAGGAGCTGCACGTGTTTGAGGACTCGGCCGCTGTCACCAGC ATGCAAATTTCTTCCAAGAGGCACCAGCTGTACGTAGCCTCGCGGAGCGCGGTGGCCCAGATCGCGTTGC ACCGCTGCGCTGCCCACGGCCGCGTCTGCACCGAATGCTGTCTGGCGCGTGACCCCTACTGCGCCTGGGA CGGGGTCGCGTGCACGCGCTTCCAGCCCAGTGCCAAGAGGCGGTTCCGGCGGCAAGACGTAAGGAATGGC GACCCCAGCACGTTGTGCTCCGGAGACTCGTCTCGTCCCGCGCTGCTGGAACACAAGGTGTTCGGCGTGG AGGGCAGCAGCGCCTTTCTGGAGTGTGAGCCCCGCTCGCTGCAGGCGCGCGTGGAGTGGACTTTCCAGCG CGCAGGGGTGACAGCCCACACCCAGGTGCTGGCAGAGGAGCGCACCGAGCGCACCGCCCGGGGACTACTG CTGCGCAGGCTGCGGCGCCGGGACTCGGGCGTGTACTTGTGCGCCGCCGTCGAGCAGGGCTTTACGCAAC CGCTGCGTCGCCTGTCGCTGCACGTGTTGAGTGCTACGCAGGCCGAACGACTGGCGCGGGCCGAGGAGGC TGCGCCCGCCGCGCCGCCGGGCCCCAAACTCTGGTACCGGGACTTTCTGCAGCTGGTGGAGCCGGGCGGA GGTGGCAGCGCGAACTCCCTGCGCATGTGCCGCCCGCAGCCTGCGCTGCAGTCACTGCCCCTGGAGTCGC GGAGAAAGGGCCGTAACCGGAGGACCCACGCCCCTGAGCCTCGCGCTGAGCGGGGGCCGCGCAGCGCAAC GCACTGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC223532 representing NM_004636
Red=Cloning site Green=Tags(s) MGRAGAAAVIPGLALLWAVGLGSAAPSPPRLRLSFQELQAWHGLQTFSLERTCCYQALLVDEERGRLFVG AENHVASLNLDNISKRAKKLAWPAPVEWREECNWAGKDIGTECMNFVKLLHAYNRTHLLACGTGAFHPTC AFVEVGHRAEEPVLRLDPGRIEDGKGKSPYDPRHRAASVLVGEELYSGVAADLMGRDFTIFRSLGQRPSL RTEPHDSRWLNEPKFVKVFWIPESENPDDDKIYFFFRETAVEAAPALGRLSVSRVGQICRNDVGGQRSLV NKWTTFLKARLVCSVPGVEGDTHFDQLQDVFLLSSRDHRTPLLYAVFSTSSSIFQGSAVCVYSMNDVRRA FLGPFAHKEGPMHQWVSYQGRVPYPRPGMCPSKTFGTFSSTKDFPDDVIQFARNHPLMYNSVLPTGGRPL FLQVGANYTFTQIAADRVAAADGHYDVLFIGTDVGTVLKVISVPKGSRPSAEGLLLEELHVFEDSAAVTS MQISSKRHQLYVASRSAVAQIALHRCAAHGRVCTECCLARDPYCAWDGVACTRFQPSAKRRFRRQDVRNG DPSTLCSGDSSRPALLEHKVFGVEGSSAFLECEPRSLQARVEWTFQRAGVTAHTQVLAEERTERTARGLL LRRLRRRDSGVYLCAAVEQGFTQPLRRLSLHVLSATQAERLARAEEAAPAAPPGPKLWYRDFLQLVEPGG GGSANSLRMCRPQPALQSLPLESRRKGRNRRTHAPEPRAERGPRSATHW TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_004636 |
| ORF Size | 2247 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_004636.3 |
| RefSeq Size | 2989 bp |
| RefSeq ORF | 2250 bp |
| Locus ID | 7869 |
| UniProt ID | Q13214 |
| Domains | Sema, IG, PSI |
| Protein Families | Secreted Protein, Transmembrane |
| Protein Pathways | Axon guidance |
| MW | 83.12 kDa |
| Gene Summary | The protein encoded by this gene belongs to the class-3 semaphorin/collapsin family, whose members function in growth cone guidance during neuronal development. This family member inhibits axonal extension and has been shown to act as a tumor suppressor by inducing apoptosis. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Feb 2014] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Human Semaphorin 3 Variants Link Melanocortin Circuit Development and Energy Balance
,van der Klaauw, AA;Croizier, S;Mendes de Oliveira, E;Stadler, LKJ;Park, S;Kong, Y;Banton, MC;Tandon, P;Hendricks, AE;Keogh, JM;Riley, SE;Papadia, S;Henning, E;Bounds, R;Bochukova, EG;Mistry, V;O'Rahilly, S;Simerly, RB;, ;, ;Minchin, JEN;Barroso, I;Jones, EY;Bouret, SG;Farooqi, IS;,
Cell
,PubMed ID 30661757
[Semaphorin 3B]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC223532L3 | Lenti-ORF clone of SEMA3B (Myc-DDK-tagged)-Human sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3B (SEMA3B), transcript variant 1 |
CNY 5,890.00 |
|
| RC223532L4 | Lenti-ORF clone of SEMA3B (mGFP-tagged)-Human sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3B (SEMA3B), transcript variant 1 |
CNY 5,890.00 |
|
| RG223532 | SEMA3B (tGFP-tagged) - Human sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3B (SEMA3B), transcript variant 1 |
CNY 7,088.00 |
|
| SC117239 | SEMA3B (untagged)-Human sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3B (SEMA3B), transcript variant 1 |
CNY 5,496.00 |
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