VNN1 (NM_004666) Human Tagged ORF Clone
CAT#: RC223462
VNN1 (Myc-DDK-tagged)-Human vanin 1 (VNN1)
ORF Plasmid: tGFP
"NM_004666" in other vectors (6)
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CNY 3,824.00
CNY 3,990.00
Cited in 1 publication. |
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | HDLCQ8; Tiff66 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC223462 representing NM_004666
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTACTCAGTTGCCAGCTTACGTGGCAATTTTGCTTTTCTATGTCTCAAGAGCCAGCTGCCAGGACA CTTTCATTGCAGCTGTTTATGAGCATGCAGCGATATTGCCCAATGCCACCCTAACACCAGTGTCTCGTGA GGAGGCTTTGGCATTAATGAATCGGAATCTGGACATTTTGGAAGGAGCGATCACATCAGCAGCAGATCAG GGTGCGCATATTATTGTGACTCCAGAAGATGCTATTTATGGCTGGAACTTCAACAGGGACTCTCTCTACC CATATTTGGAGGACATCCCAGACCCTGAAGTAAACTGGATCCCCTGTAATAATCGTAACAGATTTGGCCA GACCCCAGTACAAGAAAGACTCAGCTGCCTGGCCAAGAACAACTCTATCTATGTTGTGGCAAATATTGGG GACAAGAAGCCATGCGATACCAGTGATCCTCAGTGTCCCCCTGATGGCCGTTACCAATACAACACTGATG TGGTATTTGATTCTCAAGGAAAACTGGTGGCACGCTACCATAAGCAAAACCTTTTCATGGGTGAAAATCA ATTCAATGTACCCAAGGAGCCTGAGATTGTGACTTTCAATACCACCTTTGGAAGTTTTGGCATTTTCACA TGCTTTGATATACTCTTCCATGATCCTGCTGTTACCTTGGTGAAAGATTTCCACGTGGACACCATAGTAT TCCCAACAGCTTGGATGAATGTTTTGCCACATTTGTCAGCTGTTGAATTCCACTCAGCTTGGGCTATGGG CATGAGGGTCAATTTCCTTGCATCCAACATACATTACCCCTCAAAGAAAATGACAGGAAGTGGCATCTAT GCACCCAATTCTTCAAGAGCATTTCATTATGATATGAAGACAGAAGAGGGAAAACTCCTCCTCTCGCAAC TGGATTCCCACCCATCCCATTCTGCAGTGGTGAACTGGACTTCCTATGCCAGCAGTATAGAAGCGCTCTC ATCAGGAAACAAGGAATTTAAAGGCACTGTCTTTTTCGATGAATTCACTTTTGTGAAGCTCACAGGAGTT GCAGGAAATTATACAGTTTGTCAGAAAGATCTCTGCTGTCATTTAAGCTACAAAATGTCTGAGAACATAC CAAATGAAGTGTACGCTCTAGGGGCATTTGACGGACTGCACACTGTGGAAGGGCGCTATTATCTACAGAT TTGTACCCTGTTGAAATGTAAAACGACTAATTTAAACACTTGCGGTGACTCAGCTGAAACAGCTTCTACC AGGTTTGAAATGTTCTCCCTCAGTGGCACTTTCGGAACCCAGTATGTCTTTCCTGAGGTGTTGCTGAGTG AAAATCAGCTTGCACCTGGAGAATTTCAGGTGTCAACTGACGGACGCTTGTTTAGTCTGAAGCCAACATC CGGACCTGTCTTAACAGTAACTCTGTTTGGGAGGTTGTATGAGAAGGACTGGGCATCAAATGCTTCATCA GGCCTCACAGCACAAGCAAGAATAATAATGCTAATAGTTATAGCACCTATTGTATGCTCATTAAGTTGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC223462 representing NM_004666
Red=Cloning site Green=Tags(s) MTTQLPAYVAILLFYVSRASCQDTFIAAVYEHAAILPNATLTPVSREEALALMNRNLDILEGAITSAADQ GAHIIVTPEDAIYGWNFNRDSLYPYLEDIPDPEVNWIPCNNRNRFGQTPVQERLSCLAKNNSIYVVANIG DKKPCDTSDPQCPPDGRYQYNTDVVFDSQGKLVARYHKQNLFMGENQFNVPKEPEIVTFNTTFGSFGIFT CFDILFHDPAVTLVKDFHVDTIVFPTAWMNVLPHLSAVEFHSAWAMGMRVNFLASNIHYPSKKMTGSGIY APNSSRAFHYDMKTEEGKLLLSQLDSHPSHSAVVNWTSYASSIEALSSGNKEFKGTVFFDEFTFVKLTGV AGNYTVCQKDLCCHLSYKMSENIPNEVYALGAFDGLHTVEGRYYLQICTLLKCKTTNLNTCGDSAETAST RFEMFSLSGTFGTQYVFPEVLLSENQLAPGEFQVSTDGRLFSLKPTSGPVLTVTLFGRLYEKDWASNASS GLTAQARIIMLIVIAPIVCSLSW TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_004666 |
ORF Size | 1539 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_004666.1, NP_004657.1 |
RefSeq Size | 3109 bp |
RefSeq ORF | 1542 bp |
Locus ID | 8876 |
UniProt ID | O95497 |
Protein Pathways | Pantothenate and CoA biosynthesis |
MW | 57.02 kDa |
Gene Summary | This gene encodes a member of the vanin family of proteins, which share extensive sequence similarity with each other, and also with biotinidase. The family includes secreted and membrane-associated proteins, a few of which have been reported to participate in hematopoietic cell trafficking. No biotinidase activity has been demonstrated for any of the vanin proteins, however, they possess pantetheinase activity, which may play a role in oxidative-stress response. This protein, like its mouse homolog, is likely a GPI-anchored cell surface molecule. The mouse protein is expressed by the perivascular thymic stromal cells and regulates migration of T-cell progenitors to the thymus. This gene lies in close proximity to, and in the same transcriptional orientation as, two other vanin genes on chromosome 6q23-q24. [provided by RefSeq, Feb 2009] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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The Association of the Vanin-1 N131S Variant with Blood Pressure Is Mediated by Endoplasmic Reticulum-Associated Degradation and Loss of Function
,Wang, YJ;Tayo, BO;Bandyopadhyay, A;Wang, H;Feng, T;Franceschini, N;Tang, H;Gao, J;Sung, YJ;COGENT BP consortium, ;Elston, RC;Williams, SM;Cooper, RS;Mu, TW;Zhu, X;,
PLoS Genet.
,PubMed ID 25233454
[VNN1]
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Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC223462L1 | Lenti ORF clone of Human vanin 1 (VNN1), Myc-DDK-tagged |
CNY 6,224.00 |
|
RC223462L2 | Lenti ORF clone of Human vanin 1 (VNN1), mGFP tagged |
CNY 5,890.00 |
|
RC223462L3 | Lenti ORF clone of Human vanin 1 (VNN1), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC223462L4 | Lenti ORF clone of Human vanin 1 (VNN1), mGFP tagged |
CNY 5,890.00 |
|
RG223462 | VNN1 (tGFP-tagged) - Human vanin 1 (VNN1) |
CNY 5,424.00 |
|
SC125448 | VNN1 (untagged)-Human vanin 1 (VNN1) |
CNY 3,832.00 |