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Cyclophilin F (PPIF) (NM_005729) Human Tagged ORF Clone
CAT＃: RC223397
PPIF (Myc-DDK-tagged)-Human peptidylprolyl isomerase F (PPIF), nuclear gene encoding mitochondrial protein
ORF Plasmid: tGFP
"NM_005729" in other vectors (6)
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CNY 2,400.00
CNY 3,990.00
| Cited in 2 publications. |
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Cyp-D; CyP-M; CYP3; CypD |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC223397 representing NM_005729
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGGCGCTGCGCTGCGGCTCCCGCTGGCTCGGCCTGCTCTCCGTCCCGCGCTCCGTGCCGCTGCGCC TCCCCGCGGCCCGCGCCTGCAGCAAGGGCTCCGGCGACCCGTCCTCTTCCTCCTCCTCCGGGAACCCGCT CGTGTACCTGGACGTGGACGCCAACGGGAAGCCGCTCGGCCGCGTGGTGCTGGAGCTGAAGGCAGATGTC GTCCCAAAGACAGCTGAGAACTTCAGAGCCCTGTGCACTGGTGAGAAGGGCTTCGGCTACAAAGGCTCCA CCTTCCACAGGGTGATCCCTTCCTTCATGTGCCAGGCGGGCGACTTCACCAACCACAATGGCACAGGCGG GAAGTCCATCTACGGAAGCCGCTTTCCTGACGAGAACTTTACACTGAAGCACGTGGGGCCAGGTGTCCTG TCCATGGCTAATGCTGGTCCTAACACCAACGGCTCCCAGTTCTTCATCTGCACCATAAAGACAGACTGGT TGGATGGCAAGCATGTTGTGTTCGGTCACGTCAAAGAGGGCATGGACGTCGTGAAGAAAATAGAATCTTT CGGCTCTAAGAGTGGGAGGACATCCAAGAAGATTGTCATCACAGACTGTGGCCAGTTGAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC223397 representing NM_005729
Red=Cloning site Green=Tags(s) MLALRCGSRWLGLLSVPRSVPLRLPAARACSKGSGDPSSSSSSGNPLVYLDVDANGKPLGRVVLELKADV VPKTAENFRALCTGEKGFGYKGSTFHRVIPSFMCQAGDFTNHNGTGGKSIYGSRFPDENFTLKHVGPGVL SMANAGPNTNGSQFFICTIKTDWLDGKHVVFGHVKEGMDVVKKIESFGSKSGRTSKKIVITDCGQLS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_005729 |
| ORF Size | 621 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_005729.4 |
| RefSeq Size | 2213 bp |
| RefSeq ORF | 624 bp |
| Locus ID | 10105 |
| UniProt ID | P30405 |
| Domains | pro_isomerase |
| MW | 22.04 kDa |
| Gene Summary | The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein is part of the mitochondrial permeability transition pore in the inner mitochondrial membrane. Activation of this pore is thought to be involved in the induction of apoptotic and necrotic cell death. [provided by RefSeq, Jul 2008] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Chaperoning activity of the cyclophilin family prevents tau aggregation.
,null,
Protein science : a publication of the Protein Society
,PubMed ID 36305768
[Cyclophilin F]
|
|
Cyclophilin-D: a resident regulator of mitochondrial gene expression
,Radhakrishnan, J;Bazarek, S;Chandran, B;Gazmuri, RJ;,
FASEB J.
,PubMed ID 25837584
[Cyclophilin F]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC223397L1 | Lenti ORF clone of Human peptidylprolyl isomerase F (PPIF), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC223397L2 | Lenti ORF clone of Human peptidylprolyl isomerase F (PPIF), nuclear gene encoding mitochondrial protein, mGFP tagged |
CNY 5,890.00 |
|
| RC223397L3 | Lenti ORF clone of Human peptidylprolyl isomerase F (PPIF), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC223397L4 | Lenti ORF clone of Human peptidylprolyl isomerase F (PPIF), nuclear gene encoding mitochondrial protein, mGFP tagged |
CNY 4,800.00 |
|
| RG223397 | PPIF (tGFP-tagged) - Human peptidylprolyl isomerase F (PPIF), nuclear gene encoding mitochondrial protein |
CNY 4,370.00 |
|
| SC116591 | PPIF (untagged)-Human peptidylprolyl isomerase F (PPIF), nuclear gene encoding mitochondrial protein |
CNY 2,400.00 |
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